Restriction map for
pF1KB6005
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# NO CUT ENZYMES ( 69 enzymes) #
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Acc65I AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BbeI BcgI
BglI BglII BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
HaeII HpaI KasI KpnI MluI
NarI NdeI NotI NruI NspV
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SapI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SwaI Van91I
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 2860 GACGTC GACGT^C
AccII 11 CGCG CG^CG
AcsI 1769 GAATTC [GA]^AATT[CT]
AflII 3154 CTTAAG C^TTAAG
AflIII 2165 ACGTGT A^C[GA][CT]GT
AocI 744 CCTGAGG CC^T[ACGT]AGG
ApoI 1769 GAATTC [GA]^AATT[CT]
Asp700I 1847 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3175 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2128 CCTAGG C^CTAGG
BbrPI 2164 CACGTG CAC^GTG
BbsI 2699 GTCTTC GAAGAC(2/6)
BclI 2223 TGATCA T^GATCA
BfrI 3154 CTTAAG C^TTAAG
BlnI 2128 CCTAGG C^CTAGG
BpuAI 2699 GTCTTC GAAGAC(2/6)
BsaAI 2164 CACGTG [CT]AC^GT[GA]
BsrBI 2342 GAGCGG CCGCTC(-3/-3)
BssSI 1013 CACGAG CACGAG(-5/-1)
BstUI 11 CGCG CG^CG
Bsu36I 744 CCTGAGG CC^T[ACGT]AGG
DraIII 2585 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2860 GACGTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 665 CTCTTC CTCTTC(1/4)
Eco81I 744 CCTGAGG CC^T[ACGT]AGG
EcoRI 1769 GAATTC G^AATTC
EcoRV 2479 GATATC GAT^ATC
EcoT22I 1652 ATGCAT ATGCA^T
FbaI 2223 TGATCA T^GATCA
HincII 321 GTCGAC GT[CT]^[GA]AC
HindII 321 GTCGAC GT[CT]^[GA]AC
HindIII 545 AAGCTT A^AGCTT
Ksp632I 665 CTCTTC CTCTTC(1/4)
KspI 10 CCGCGG CCGC^GG
MfeI 1883 CAATTG C^AATTG
MunI 1883 CAATTG C^AATTG
MvnI 11 CGCG CG^CG
NheI 1963 GCTAGC G^CTAGC
NsiI 1652 ATGCAT ATGCA^T
PflFI 3175 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2164 CACGTG CAC^GTG
PmlI 2164 CACGTG CAC^GTG
PpuMI 1729 GGGACCC [GA]G^G[AT]CC[CT]
SacI 890 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SalI 321 GTCGAC G^TCGAC
ScaI 1108 AGTACT AGT^ACT
SexAI 2586 ACCTGGT A^CC[AT]GGT
SmaI 263 CCCGGG CCC^GGG
SspI 1457 AATATT AAT^ATT
StuI 2131 AGGCCT AGG^CCT
Tth111I 3175 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2985 TCTAGA T^CTAGA
XmaCI 263 CCCGGG C^CCGGG
XmaI 263 CCCGGG C^CCGGG
XmnI 1847 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 321 GTCGAC GT^[AC][GT]AC
1611 GTCTAC
AccIII 1378 TCCGGA T^CCGGA
2012 TCCGGA
AvaII 1730 GGACC G^G[AT]CC
2346 GGTCC
BamHI 1331 GGATCC G^GATCC
1679 GGATCC
BbvCI 1834 CCTCAGC CCTCAGC(-5/-2)
2065 CCTCAGC
BlpI 123 GCTGAGC GC^T[ACGT]AGC
781 GCTCAGC
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
781 GCTCAGC
BsaBI 1368 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3058 GATGATGATC
BseAI 1378 TCCGGA T^CCGGA
2012 TCCGGA
BspEI 1378 TCCGGA T^CCGGA
2012 TCCGGA
BspHI 611 TCATGA T^CATGA
2483 TCATGA
BsrFI 25 GCCGGC [GA]^CCGG[CT]
1716 GCCGGC
BstAPI 700 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1654 GCATCGCATGC
BstEII 513 GGTTACC G^GT[ACGT]ACC
2123 GGTCACC
BstPI 513 GGTTACC G^GT[ACGT]ACC
2123 GGTCACC
CelII 123 GCTGAGC GC^T[ACGT]AGC
781 GCTCAGC
CfoI 922 GCGC GCG^C
2721 GCGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
1716 GCCGGC
EcoO65I 513 GGTTACC G^GT[ACGT]ACC
2123 GGTCACC
HhaI 922 GCGC GCG^C
2721 GCGC
HinP1I 922 GCGC G^CGC
2721 GCGC
MamI 1368 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3058 GATGATGATC
MroI 1378 TCCGGA T^CCGGA
2012 TCCGGA
NaeI 25 GCCGGC GCC^GGC
1716 GCCGGC
NgoMIV 25 GCCGGC G^CCGGC
1716 GCCGGC
PaeR7I 692 CTCGAG C^TCGAG
2090 CTCGAG
PleI 3145 GACTC GAGTC(4/5)
2697 GAGTC
RcaI 611 TCATGA T^CATGA
2483 TCATGA
SbfI 2134 CCTGCAGG CCTGCA^GG
3018 CCTGCAGG
SphI 924 GCATGC GCATG^C
1659 GCATGC
Sse8387I 2134 CCTGCAGG CCTGCA^GG
3018 CCTGCAGG
XcmI 2027 CCACAGATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2693 CCAAGAGTCTTCTGG
XhoI 692 CTCGAG C^TCGAG
2090 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI BalI
BanI BanII BbvI BciVI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII