Restriction map for
pF1KB5695
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BglI BglII BlnI BsaBI BsaHI
BsgI BsiWI BspDI BspHI BspLU11I
BspMI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI Hin1I KasI KpnI KspI
MamI MfeI MunI NarI NheI
NotI NruI NsiI NspV PacI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI SphI
Sse8387I SspI StuI SwaI TfiI
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccIII 929 TCCGGA T^CCGGA
AflIII 161 ACGCGT A^C[GA][CT]GT
AgeI 1891 ACCGGT A^CCGGT
AhdI 1464 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1490 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 103 GGGCCC GGGCC^C
Asp700I 1774 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1464 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1295 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1789 CTCGAG C^[CT]CG[GA]G
BalI 1073 TGGCCA TGG^CCA
BanI 1321 GGTGCC G^G[CT][GA]CC
BbsI 598 GAAGAC GAAGAC(2/6)
BbvCI 1558 CCTCAGC CCTCAGC(-5/-2)
BclI 137 TGATCA T^GATCA
BlpI 1102 GCTCAGC GC^T[ACGT]AGC
BpmI 1796 CTCCAG CTGGAG(16/14)
Bpu1102I 1102 GCTCAGC GC^T[ACGT]AGC
BpuAI 598 GAAGAC GAAGAC(2/6)
BsaAI 40 TACGTG [CT]AC^GT[GA]
BseAI 929 TCCGGA T^CCGGA
BsiEI 98 CGGCCG CG[GA][CT]^CG
BsmI 479 GAATGC GAATGC(1/-1)
BsoBI 1789 CTCGAG C^[CT]CG[GA]G
BspEI 929 TCCGGA T^CCGGA
BsrBI 147 CCGCTC CCGCTC(-3/-3)
BssSI 1421 CACGAG CACGAG(-5/-1)
BstAPI 1489 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 624 GGATCT [GA]^GATC[CT]
CelII 1102 GCTCAGC GC^T[ACGT]AGC
DraII 47 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1436 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 26 CCATGG C^C[GA][CT]GG
EagI 98 CGGCCG C^GGCCG
Eam1105I 1464 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1114 GAAGAG CTCTTC(1/4)
EclXI 98 CGGCCG C^GGCCG
Eco52I 98 CGGCCG C^GGCCG
EcoNI 1210 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 47 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 565 GAATTC G^AATTC
FauI 143 CCCGC CCCGC(4/6)
FbaI 137 TGATCA T^GATCA
HindIII 708 AAGCTT A^AGCTT
HpaI 1879 GTTAAC GTT^AAC
Ksp632I 1114 GAAGAG CTCTTC(1/4)
MflI 624 GGATCT [GA]^GATC[CT]
MluI 161 ACGCGT A^CGCGT
MluNI 1073 TGGCCA TGG^CCA
MroI 929 TCCGGA T^CCGGA
MscI 1073 TGGCCA TGG^CCA
NaeI 183 GCCGGC GCC^GGC
NcoI 26 CCATGG C^CATGG
NdeI 1783 CATATG CA^TATG
NgoMIV 183 GCCGGC G^CCGGC
NspI 1748 GCATGT [GA]CATG^[CT]
PaeR7I 1789 CTCGAG C^TCGAG
PflFI 1295 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1891 ACCGGT A^CCGGT
PpuMI 47 GGGACCT [GA]G^G[AT]CC[CT]
SapI 1114 GAAGAGC GCTCTTC(1/4)
ScaI 1390 AGTACT AGT^ACT
SgrAI 1890 CACCGGTG C[GA]^CCGG[CT]G
SpeI 961 ACTAGT A^CTAGT
Tth111I 1295 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1789 CTCGAG C^TCGAG
XhoII 624 GGATCT [GA]^GATC[CT]
XmnI 1774 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 162 CGCG CG^CG
192 CGCG
AspHI 763 GAGCTC G[AT]GC[AT]^C
794 GAGCTC
AvaII 48 GGACC G^G[AT]CC
1471 GGTCC
BsaI 1398 GAGACC GGTCTC(1/5)
257 GGTCTC
BsaWI 929 TCCGGA [AT]^CCGG[AT]
1891 ACCGGT
BseRI 1020 CTCCTC GAGGAG(10/8)
1661 CTCCTC
BsiHKAI 763 GAGCTC G[AT]GC[AT]^C
794 GAGCTC
BsmBI 925 CGTCTC CGTCTC(1/5)
436 GAGACG
BsmFI 47 GGGAC GGGAC(10/14)
131 GGGAC
Bsp1407I 426 TGTACA T^GTACA
1614 TGTACA
BsrFI 183 GCCGGC [GA]^CCGG[CT]
1891 ACCGGT
BsrGI 426 TGTACA T^GTACA
1614 TGTACA
BstNI 873 CCAGG CC^[AT]GG
1122 CCAGG
BstUI 162 CGCG CG^CG
192 CGCG
Cfr10I 183 GCCGGC [GA]^CCGG[CT]
1891 ACCGGT
EaeI 98 CGGCCG [CT]^GGCC[GA]
1073 TGGCCA
EcoRII 873 CCAGG ^CC[AT]GG
1122 CCAGG
HaeII 614 AGCGCC [GA]GCGC^[CT]
1118 AGCGCC
HgaI 190 GACGC GACGC(5/10)
438 GACGC
HincII 1431 GTCAAC GT[CT]^[GA]AC
1879 GTTAAC
HindII 1431 GTCAAC GT[CT]^[GA]AC
1879 GTTAAC
MvaI 873 CCAGG CC^[AT]GG
1122 CCAGG
MvnI 162 CGCG CG^CG
192 CGCG
SacI 763 GAGCTC GAGCT^C
794 GAGCTC
SspBI 426 TGTACA T^GTACA
1614 TGTACA
TaqI 792 TCGA T^CGA
1790 TCGA
Tsp45I 64 GTGAC ^GT[GC]AC
1803 GTCAC
TthHB8I 792 TCGA T^CGA
1790 TCGA
XcmI 17 CCAGCTACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1297 CCAAGTCCTCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI BanII BbvI BcnI
BfaI BfrI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I Cac8I CfoI Cfr13I DdeI
DpnII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp509I TspRI