Restriction map for
pF1KB5666
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AclI AflII Aor51HI AscI AviII
AvrII BbrPI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KspI
MaeI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI ScaI SfuI
SnaBI SpeI SphI SspBI SspI
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 352 GACGTC GACGT^C
Acc65I 2528 GGTACC G^GTACC
AccI 2386 GTCTAC GT^[AC][GT]AC
AccII 1324 CGCG CG^CG
AcsI 1735 GAATTC [GA]^AATT[CT]
AflIII 1745 ACGTGT A^C[GA][CT]GT
AgeI 1580 ACCGGT A^CCGGT
AhdI 1108 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1449 CCTCAGG CC^T[ACGT]AGG
ApoI 1735 GAATTC [GA]^AATT[CT]
AseI 1984 ATTAAT AT^TAAT
AsnI 1984 ATTAAT AT^TAAT
Asp700I 654 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2528 GGTACC G^GTACC
AspEI 1108 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2632 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2801 CCCGGG C^[CT]CG[GA]G
BciVI 1662 GGATAC GTATCC(6/5)
BsaBI 897 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2801 CCCGGG C^[CT]CG[GA]G
BstUI 1324 CGCG CG^CG
Bsu36I 1449 CCTCAGG CC^T[ACGT]AGG
DraIII 403 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 729 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1108 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1449 CCTCAGG CC^T[ACGT]AGG
EcoRI 1735 GAATTC G^AATTC
HindIII 2656 AAGCTT A^AGCTT
KpnI 2528 GGTACC GGTAC^C
MamI 897 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1324 CGCG CG^CG
NcoI 1058 CCATGG C^CATGG
PflFI 2632 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1580 ACCGGT A^CCGGT
PleI 1929 GACTC GAGTC(4/5)
PshBI 1984 ATTAAT AT^TAAT
SbfI 1560 CCTGCAGG CCTGCA^GG
SfiI 681 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1050 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2801 CCCGGG CCC^GGG
SmlI 496 CTCAAG C^T[CT][GA]AG
Sse8387I 1560 CCTGCAGG CCTGCA^GG
Tsp509I 1736 AATT ^AATT
Tth111I 2632 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2801 CCCGGG C^CCGGG
XmaI 2801 CCCGGG C^CCGGG
XmnI 654 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 899 TCCGGA T^CCGGA
2561 TCCGGA
Alw44I 69 GTGCAC G^TGCAC
490 GTGCAC
AlwNI 1492 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2746 CAGCAGCTG
ApaLI 69 GTGCAC G^TGCAC
490 GTGCAC
BamHI 44 GGATCC G^GATCC
2516 GGATCC
BbeI 1054 GGCGCC GGCGC^C
1438 GGCGCC
BbsI 778 GAAGAC GAAGAC(2/6)
2826 GAAGAC
BbvCI 158 CCTCAGC CCTCAGC(-5/-2)
390 CCTCAGC
BglI 682 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
922 GCCATTGCGGC
BpuAI 778 GAAGAC GAAGAC(2/6)
2826 GAAGAC
BseAI 899 TCCGGA T^CCGGA
2561 TCCGGA
BspEI 899 TCCGGA T^CCGGA
2561 TCCGGA
BspHI 1161 TCATGA T^CATGA
1616 TCATGA
BsrBI 2130 CCGCTC CCGCTC(-3/-3)
1138 GAGCGG
BstAPI 1333 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2273 GCACCGCCTGC
BstEII 1077 GGTGACC G^GT[ACGT]ACC
1194 GGTGACC
BstPI 1077 GGTGACC G^GT[ACGT]ACC
1194 GGTGACC
BstXI 23 CCAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
185 CCACCACCTTGG
EcoO65I 1077 GGTGACC G^GT[ACGT]ACC
1194 GGTGACC
KasI 1054 GGCGCC G^GCGCC
1438 GGCGCC
MroI 899 TCCGGA T^CCGGA
2561 TCCGGA
MslI 1599 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1653 CATCACGGTG
NarI 1054 GGCGCC GG^CGCC
1438 GGCGCC
NspI 743 ACATGC [GA]CATG^[CT]
2075 ACATGC
PflMI 1199 CCATAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2210 CCAACAACTGG
PvuII 1933 CAGCTG CAG^CTG
2749 CAGCTG
RcaI 1161 TCATGA T^CATGA
1616 TCATGA
SexAI 482 ACCTGGT A^CC[AT]GGT
1190 ACCTGGT
SfoI 1054 GGCGCC GGC^GCC
1438 GGCGCC
Van91I 1199 CCATAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2210 CCAACAACTGG
XcmI 473 CCACCGAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
827 CCACGTACTTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AspHI AvaII BalI BanI
BanII BbvI BcgI BcnI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII