Restriction map for
pF1KB5626
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcnI BfrI BglI BlnI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FseI FspI HaeII HgaI HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1169 GACGTC GACGT^C
AccII 78 CGCG CG^CG
AcyI 1169 GACGTC G[GA]^CG[CT]C
AocI 1212 CCTCAGG CC^T[ACGT]AGG
ApaI 1158 GGGCCC GGGCC^C
Asp700I 558 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 472 GTATCC GTATCC(6/5)
BclI 341 TGATCA T^GATCA
BglII 1351 AGATCT A^GATCT
BlpI 1282 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1282 GCTGAGC GC^T[ACGT]AGC
BsaAI 934 TACGTA [CT]AC^GT[GA]
BsaHI 1169 GACGTC G[GA]^CG[CT]C
BsmFI 523 GGGAC GGGAC(10/14)
BspMI 145 GCAGGT ACCTGC(4/8)
BsrDI 873 CATTGC GCAATG(2/0)
BsrFI 739 ACCGGC [GA]^CCGG[CT]
BssSI 1193 CTCGTG CACGAG(-5/-1)
BstUI 78 CGCG CG^CG
Bsu36I 1212 CCTCAGG CC^T[ACGT]AGG
CelII 1282 GCTGAGC GC^T[ACGT]AGC
CfoI 9 GCGC GCG^C
Cfr10I 739 ACCGGC [GA]^CCGG[CT]
DsaI 77 CCGCGG C^C[GA][CT]GG
EarI 720 GAAGAG CTCTTC(1/4)
Eco81I 1212 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1317 ATGCAT ATGCA^T
FauI 82 GCGGG CCCGC(4/6)
FbaI 341 TGATCA T^GATCA
HhaI 9 GCGC GCG^C
Hin1I 1169 GACGTC G[GA]^CG[CT]C
HinP1I 9 GCGC G^CGC
Ksp632I 720 GAAGAG CTCTTC(1/4)
KspI 77 CCGCGG CCGC^GG
MvnI 78 CGCG CG^CG
NdeI 437 CATATG CA^TATG
NsiI 1317 ATGCAT ATGCA^T
PaeR7I 615 CTCGAG C^TCGAG
PstI 143 CTGCAG CTGCA^G
SacII 77 CCGCGG CCGC^GG
SfcI 143 CTGCAG C^T[GA][CT]AG
SmlI 615 CTCGAG C^T[CT][GA]AG
SnaBI 934 TACGTA TAC^GTA
SpeI 156 ACTAGT A^CTAGT
XcmI 1361 CCATTCCCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 615 CTCGAG C^TCGAG
XmnI 558 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 662 GTAC GT^AC
714 GTAC
AlwI 1310 GATCC GGATC(4/5)
1326 GATCC
AvaI 615 CTCGAG C^[CT]CG[GA]G
907 CCCGAG
BanII 93 GAGCCC G[GA]GC[CT]^C
1158 GGGCCC
BbsI 444 GTCTTC GAAGAC(2/6)
830 GTCTTC
BcgI 743 GCATCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
837 GCAGAGCCTTCG
BfaI 157 CTAG C^TAG
1052 CTAG
BpuAI 444 GTCTTC GAAGAC(2/6)
830 GTCTTC
BsaJI 77 CCGCGG C^C[ACGT][ACGT]GG
274 CCAGGG
BsoBI 615 CTCGAG C^[CT]CG[GA]G
907 CCCGAG
DraII 1157 GGGGCCC [GA]G^G[ACGT]CC[CT]
1188 AGGCCCT
EcoO109I 1157 GGGGCCC [GA]G^G[ACGT]CC[CT]
1188 AGGCCCT
MaeI 157 CTAG C^TAG
1052 CTAG
MseI 296 TTAA T^TAA
1175 TTAA
NspI 1218 GCATGC [GA]CATG^[CT]
1319 GCATGC
PvuII 919 CAGCTG CAG^CTG
1059 CAGCTG
RsaI 662 GTAC GT^AC
714 GTAC
SphI 1218 GCATGC GCATG^C
1319 GCATGC
TaqI 234 TCGA T^CGA
616 TCGA
Tru9I 296 TTAA T^TAA
1175 TTAA
Tsp45I 1004 GTCAC ^GT[GC]AC
1039 GTCAC
Tsp509I 227 AATT ^AATT
350 AATT
TthHB8I 234 TCGA T^CGA
616 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI TspRI
XhoII