Restriction map for
pF1KB5576
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 94 CGCG CG^CG
AfaI 350 GTAC GT^AC
ApaI 241 GGGCCC GGGCC^C
AspI 154 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 286 GGCACC G^G[CT][GA]CC
BanII 241 GGGCCC G[GA]GC[CT]^C
BbsI 322 GAAGAC GAAGAC(2/6)
BcgI 96 CGACTGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 59 GCCTCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 241 GGGCCC G[AGT]GC[ACT]^C
BpuAI 322 GAAGAC GAAGAC(2/6)
BseRI 269 GAGGAG GAGGAG(10/8)
BsmBI 90 GAGACG CGTCTC(1/5)
Bsp1286I 241 GGGCCC G[AGT]GC[ACT]^C
BspMI 294 ACCTGC ACCTGC(4/8)
BstAPI 44 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 94 CGCG CG^CG
CfoI 106 GCGC GCG^C
DpnII 471 GATC ^GATC
DsaI 236 CCACGG C^C[GA][CT]GG
EarI 61 CTCTTC CTCTTC(1/4)
EcoNI 361 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 210 CCTTGG C^C[AT][AT]GG
HgaI 92 GACGC GACGC(5/10)
HhaI 106 GCGC GCG^C
HinP1I 106 GCGC G^CGC
HinfI 406 GACTC G^A[ACGT]TC
Ksp632I 61 CTCTTC CTCTTC(1/4)
MboI 471 GATC ^GATC
MspA1I 85 CAGCTG C[AC]G^C[GT]G
MvnI 94 CGCG CG^CG
NaeI 464 GCCGGC GCC^GGC
NdeII 471 GATC ^GATC
NgoMIV 464 GCCGGC G^CCGGC
PflFI 154 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 406 GACTC GAGTC(4/5)
PpuMI 385 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 304 CTGCAG CTGCA^G
PvuII 85 CAGCTG CAG^CTG
RsaI 350 GTAC GT^AC
Sau3AI 471 GATC ^GATC
SfcI 304 CTGCAG C^T[GA][CT]AG
StuI 47 AGGCCT AGG^CCT
StyI 210 CCTTGG C^C[AT][AT]GG
Tth111I 154 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 86 AGCT AG^CT
224 AGCT
BcnI 245 CCCGG CC^[GC]GG
255 CCGGG
BpmI 175 CTGGAG CTGGAG(16/14)
401 CTGGAG
BsmFI 310 GGGAC GGGAC(10/14)
387 GTCCC
BsrFI 9 ACCGGC [GA]^CCGG[CT]
464 GCCGGC
BsrI 174 ACTGG ACTGG(1/-1)
70 CCAGT
Cfr10I 9 ACCGGC [GA]^CCGG[CT]
464 GCCGGC
FauI 115 GCGGG CCCGC(4/6)
204 GCGGG
NciI 245 CCCGG CC^[GC]GG
255 CCGGG
SfaNI 103 GATGC GCATC(5/9)
122 GCATC
TaqI 152 TCGA T^CGA
417 TCGA
TthHB8I 152 TCGA T^CGA
417 TCGA
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AvaII BbvI BsaJI BsiYI
BslI BsmAI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboII
MnlI MspI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TseI Tsp45I
TspRI