Restriction map for
pF1KB5528
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BanI BbeI BbrPI BglI
BglII BlnI BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflII 508 CTTAAG C^TTAAG
AlwNI 3526 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2498 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 77 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 24 GGATCC G^GATCC
BbvCI 2086 GCTGAGG CCTCAGC(-5/-2)
BciVI 1999 GTATCC GTATCC(6/5)
BclI 4254 TGATCA T^GATCA
BfrI 508 CTTAAG C^TTAAG
BlpI 145 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 145 GCTGAGC GC^T[ACGT]AGC
BsaWI 2045 ACCGGA [AT]^CCGG[AT]
BspDI 998 ATCGAT AT^CGAT
BsrFI 2779 GCCGGC [GA]^CCGG[CT]
CelII 145 GCTGAGC GC^T[ACGT]AGC
CfoI 651 GCGC GCG^C
Cfr10I 2779 GCCGGC [GA]^CCGG[CT]
ClaI 998 ATCGAT AT^CGAT
DraI 213 TTTAAA TTT^AAA
DraIII 2858 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 230 GACAGATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 153 GATATC GAT^ATC
EcoT22I 2017 ATGCAT ATGCA^T
FauI 4047 CCCGC CCCGC(4/6)
FbaI 4254 TGATCA T^GATCA
HhaI 651 GCGC GCG^C
HinP1I 651 GCGC G^CGC
HincII 457 GTTAAC GT[CT]^[GA]AC
HindII 457 GTTAAC GT[CT]^[GA]AC
HindIII 962 AAGCTT A^AGCTT
HpaI 457 GTTAAC GTT^AAC
NaeI 2779 GCCGGC GCC^GGC
NgoMIV 2779 GCCGGC G^CCGGC
NsiI 2017 ATGCAT ATGCA^T
PflFI 77 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1891 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3642 GAGCTC GAGCT^C
SpeI 2422 ACTAGT A^CTAGT
SspI 1663 AATATT AAT^ATT
Tth111I 77 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2397 TCTAGA T^CTAGA
XmnI 2498 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AocI 335 CCTTAGG CC^T[ACGT]AGG
2747 CCTCAGG
BcgI 2535 GCAGTCTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3997 GCAATGATGTCG
BcnI 1893 CCGGG CC^[GC]GG
3485 CCCGG
BsmBI 108 GAGACG CGTCTC(1/5)
389 GAGACG
BspHI 1439 TCATGA T^CATGA
3476 TCATGA
BsrDI 2879 CATTGC GCAATG(2/0)
3997 GCAATG
BssSI 695 CACGAG CACGAG(-5/-1)
3464 CACGAG
BstXI 2969 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3197 CCACAGGCCTGG
Bsu36I 335 CCTTAGG CC^T[ACGT]AGG
2747 CCTCAGG
Eco81I 335 CCTTAGG CC^T[ACGT]AGG
2747 CCTCAGG
MaeII 237 ACGT A^CGT
948 ACGT
MspA1I 2084 CAGCTG C[AC]G^C[GT]G
3166 CAGCTG
NciI 1893 CCGGG CC^[GC]GG
3485 CCCGG
PflMI 3934 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3964 CCAGCCTTTGG
PvuII 2084 CAGCTG CAG^CTG
3166 CAGCTG
RcaI 1439 TCATGA T^CATGA
3476 TCATGA
SexAI 452 ACCTGGT A^CC[AT]GGT
2904 ACCAGGT
SphI 1340 GCATGC GCATG^C
4238 GCATGC
Van91I 3934 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3964 CCAGCCTTTGG
XcmI 1700 CCAAGAGTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2849 CCAGACCTGCACTGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AluI Alw44I AlwI
ApaLI ApoI AspHI AvaII BalI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII