Restriction map for
pF1KB5493
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsiWI
BsmBI BspDI BspLU11I BssHII Bst1107I
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MfeI MflI
MluI MunI MvnI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 2203 GGTACC G^GTACC
AcyI 24 GGCGCC G[GA]^CG[CT]C
AlwI 2152 GATCC GGATC(4/5)
Aor51HI 219 AGCGCT AGC^GCT
Asp718I 2203 GGTACC G^GTACC
AspI 1682 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1001 CTCGGG C^[CT]CG[GA]G
BbeI 24 GGCGCC GGCGC^C
BsaAI 715 TACGTG [CT]AC^GT[GA]
BsaBI 2146 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 24 GGCGCC G[GA]^CG[CT]C
BsaI 1687 GGTCTC GGTCTC(1/5)
BsiEI 2349 CGACCG CG[GA][CT]^CG
BsoBI 1001 CTCGGG C^[CT]CG[GA]G
Bsp1407I 969 TGTACA T^GTACA
BsrGI 969 TGTACA T^GTACA
BssSI 175 CTCGTG CACGAG(-5/-1)
BstAPI 1827 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2417 TTCGAA TT^CGAA
Eco47III 219 AGCGCT AGC^GCT
EcoNI 1698 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 671 ATGCAT ATGCA^T
Hin1I 24 GGCGCC G[GA]^CG[CT]C
KasI 24 GGCGCC G^GCGCC
KpnI 2203 GGTACC GGTAC^C
MamI 2146 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1319 TTAA T^TAA
NaeI 896 GCCGGC GCC^GGC
NarI 24 GGCGCC GG^CGCC
NcoI 2183 CCATGG C^CATGG
NgoMIV 896 GCCGGC G^CCGGC
NsiI 671 ATGCAT ATGCA^T
NspI 644 ACATGC [GA]CATG^[CT]
NspV 2417 TTCGAA TT^CGAA
PflFI 1682 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1330 GCTCTTC GCTCTTC(1/4)
SfoI 24 GGCGCC GGC^GCC
SfuI 2417 TTCGAA TT^CGAA
SgrAI 26 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 969 TGTACA T^GTACA
Tru9I 1319 TTAA T^TAA
Tth111I 1682 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 1364 TCCGGA T^CCGGA
2057 TCCGGA
AcsI 289 AAATTT [GA]^AATT[CT]
2134 AAATTC
ApoI 289 AAATTT [GA]^AATT[CT]
2134 AAATTC
BsaWI 1364 TCCGGA [AT]^CCGG[AT]
2057 TCCGGA
BseAI 1364 TCCGGA T^CCGGA
2057 TCCGGA
BspEI 1364 TCCGGA T^CCGGA
2057 TCCGGA
BspHI 465 TCATGA T^CATGA
2142 TCATGA
BsrBI 84 GAGCGG CCGCTC(-3/-3)
94 GAGCGG
BstXI 1772 CCAACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2183 CCATGGGCATGG
HaeII 24 GGCGCC [GA]GCGC^[CT]
219 AGCGCT
MaeII 716 ACGT A^CGT
2494 ACGT
MroI 1364 TCCGGA T^CCGGA
2057 TCCGGA
NdeI 674 CATATG CA^TATG
1796 CATATG
PflMI 301 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2541 CCAGGCAGTGG
PvuII 1229 CAGCTG CAG^CTG
1831 CAGCTG
RcaI 465 TCATGA T^CATGA
2142 TCATGA
SacI 1328 GAGCTC GAGCT^C
1645 GAGCTC
SmlI 418 CTCAAG C^T[CT][GA]AG
1648 CTCAAG
Tsp45I 1376 GTGAC ^GT[GC]AC
1549 GTCAC
Van91I 301 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2541 CCAGGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I XcmI