Restriction map for
pF1KB5456
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
HgaI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StyI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 438 GACGTC GACGT^C
AcyI 438 GACGTC G[GA]^CG[CT]C
AflIII 1236 ACATGT A^C[GA][CT]GT
AlwI 1055 GGATC GGATC(4/5)
ApaI 518 GGGCCC GGGCC^C
Asp700I 267 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 597 GGACC G^G[AT]CC
AviII 605 TGCGCA TGC^GCA
BalI 824 TGGCCA TGG^CCA
BanI 8 GGCACC G^G[CT][GA]CC
BbvCI 406 GCTGAGG CCTCAGC(-5/-2)
BglII 783 AGATCT A^GATCT
BsaHI 438 GACGTC G[GA]^CG[CT]C
BsaWI 894 TCCGGT [AT]^CCGG[AT]
BsmFI 1190 GTCCC GGGAC(10/14)
BspDI 841 ATCGAT AT^CGAT
BspLU11I 1236 ACATGT A^CATGT
BsrBI 757 GAGCGG CCGCTC(-3/-3)
BstF5I 192 CATCC GGATG(2/0)
BstXI 299 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 783 AGATCT [GA]^GATC[CT]
ClaI 841 ATCGAT AT^CGAT
DraIII 583 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 916 GATATC GAT^ATC
FokI 192 CATCC GGATG(9/13)
FspI 605 TGCGCA TGC^GCA
Hin1I 438 GACGTC G[GA]^CG[CT]C
MaeIII 436 GTGAC ^GT[ACGT]AC
MflI 783 AGATCT [GA]^GATC[CT]
MluNI 824 TGGCCA TGG^CCA
MscI 824 TGGCCA TGG^CCA
SbfI 984 CCTGCAGG CCTGCA^GG
SphI 608 GCATGC GCATG^C
Sse8387I 984 CCTGCAGG CCTGCA^GG
StuI 416 AGGCCT AGG^CCT
Tsp45I 436 GTGAC ^GT[GC]AC
XcmI 299 CCAGGAAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 783 AGATCT [GA]^GATC[CT]
XmnI 267 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 76 GTAC GT^AC
1017 GTAC
Aor51HI 155 AGCGCT AGC^GCT
221 AGCGCT
BsmI 496 GAATGC GAATGC(1/-1)
770 GAATGC
BstAPI 357 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
582 GCACAGCGTGC
Eco47III 155 AGCGCT AGC^GCT
221 AGCGCT
MaeII 74 ACGT A^CGT
439 ACGT
PflMI 501 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAGAGCGTGG
RsaI 76 GTAC GT^AC
1017 GTAC
SacI 178 GAGCTC GAGCT^C
979 GAGCTC
SapI 18 GAAGAGC GCTCTTC(1/4)
708 GCTCTTC
SfaNI 187 GATGC GCATC(5/9)
389 GATGC
SmaI 521 CCCGGG CCC^GGG
953 CCCGGG
TfiI 171 GAATC G^A[AT]TC
210 GATTC
TspRI 48 CAGTG CA[GC]TG[ACGT][ACGT]^
753 CAGTG
Van91I 501 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAGAGCGTGG
XmaCI 521 CCCGGG C^CCGGG
953 CCCGGG
XmaI 521 CCCGGG C^CCGGG
953 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI BanII BbvI BcnI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
Sau3AI Sau96I ScrFI SfcI SfiI
TaqI TseI TthHB8I