Restriction map for
pF1KB5441
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AvrII BamHI BbeI BbsI
BbvCI BcgI BclI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaHI BseAI BseRI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI HaeII Hin1I HpaI
KasI KspI MaeI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 178 GGTACC G^GTACC
AcsI 983 GAATTC [GA]^AATT[CT]
AflIII 804 ACACGT A^C[GA][CT]GT
ApaI 193 GGGCCC GGGCC^C
ApoI 983 GAATTC [GA]^AATT[CT]
Asp718I 178 GGTACC G^GTACC
AspHI 562 GAGCAC G[AT]GC[AT]^C
AviII 378 TGCGCA TGC^GCA
BanI 178 GGTACC G^G[CT][GA]CC
BbrPI 117 CACGTG CAC^GTG
BciVI 676 GGATAC GTATCC(6/5)
BglI 328 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 117 CACGTG [CT]AC^GT[GA]
BsaBI 360 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 299 GAGACC GGTCTC(1/5)
BsgI 534 GTGCAG GTGCAG(16/14)
BsiHKAI 562 GAGCAC G[AT]GC[AT]^C
BsmBI 693 CGTCTC CGTCTC(1/5)
BspMI 410 ACCTGC ACCTGC(4/8)
CfoI 379 GCGC GCG^C
DdeI 937 CTCAG C^T[ACGT]AG
DraI 928 TTTAAA TTT^AAA
DsaI 743 CCGTGG C^C[GA][CT]GG
EarI 165 GAAGAG CTCTTC(1/4)
EcoRI 983 GAATTC G^AATTC
FspI 378 TGCGCA TGC^GCA
HgaI 692 GCGTC GACGC(5/10)
HhaI 379 GCGC GCG^C
HinP1I 379 GCGC G^CGC
KpnI 178 GGTACC GGTAC^C
Ksp632I 165 GAAGAG CTCTTC(1/4)
MamI 360 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 165 GAAGA GAAGA(8/7)
MseI 929 TTAA T^TAA
MslI 370 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 890 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 117 CACGTG CAC^GTG
PmlI 117 CACGTG CAC^GTG
ScaI 1001 AGTACT AGT^ACT
StuI 495 AGGCCT AGG^CCT
Tru9I 929 TTAA T^TAA
Van91I 890 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 840 CCGC CCGC(-3/-1)
403 GCGG
AlwI 359 GGATC GGATC(4/5)
948 GGATC
AvaII 228 GGACC G^G[AT]CC
408 GGACC
BanII 193 GGGCCC G[GA]GC[CT]^C
306 GGGCTC
BglII 626 AGATCT A^GATCT
725 AGATCT
BsaWI 356 ACCGGA [AT]^CCGG[AT]
811 ACCGGA
BsmAI 299 GAGAC GTCTC(1/5)
694 GTCTC
BsmFI 440 GGGAC GGGAC(10/14)
278 GTCCC
HindIII 246 AAGCTT A^AGCTT
470 AAGCTT
HphI 913 GGTGA GGTGA(8/7)
809 TCACC
MaeIII 808 GTCAC ^GT[ACGT]AC
914 GTGAC
NspI 418 ACATGC [GA]CATG^[CT]
431 GCATGT
PpuMI 227 AGGACCT [GA]G^G[AT]CC[CT]
407 AGGACCT
SfcI 642 CTACAG C^T[GA][CT]AG
687 CTACAG
TaqI 251 TCGA T^CGA
962 TCGA
Tsp45I 808 GTCAC ^GT[GC]AC
914 GTGAC
Tsp509I 682 AATT ^AATT
984 AATT
TthHB8I 251 TCGA T^CGA
962 TCGA
XcmI 303 CCAGGGCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
836 CCATCCGCATCCTGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI BalI BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
ItaI MaeII MboI MflI MluNI
MnlI MscI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI TspRI XhoII