Restriction map for
pF1KB5396
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FauI
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 917 GACGCC G[GA]^CG[CT]C
AflIII 267 ACATGT A^C[GA][CT]GT
Asp700I 142 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1682 CTCGGG C^[CT]CG[GA]G
BanII 224 GAGCTC G[GA]GC[CT]^C
BlpI 1798 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1798 GCTCAGC GC^T[ACGT]AGC
BsaHI 917 GACGCC G[GA]^CG[CT]C
BsaWI 604 TCCGGT [AT]^CCGG[AT]
BsgI 653 CTGCAC GTGCAG(16/14)
BsoBI 1682 CTCGGG C^[CT]CG[GA]G
BspLU11I 267 ACATGT A^CATGT
BsrDI 1700 GCAATG GCAATG(2/0)
BsrFI 920 GCCGGC [GA]^CCGG[CT]
BstAPI 190 GCAATTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1798 GCTCAGC GC^T[ACGT]AGC
CfoI 1254 GCGC GCG^C
Cfr10I 920 GCCGGC [GA]^CCGG[CT]
EcoNI 1564 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 793 GAATTC G^AATTC
HaeII 1253 AGCGCC [GA]GCGC^[CT]
HhaI 1254 GCGC GCG^C
Hin1I 917 GACGCC G[GA]^CG[CT]C
HinP1I 1254 GCGC G^CGC
MfeI 191 CAATTG C^AATTG
MspA1I 1157 CAGCTG C[AC]G^C[GT]G
MunI 191 CAATTG C^AATTG
NaeI 920 GCCGGC GCC^GGC
NgoMIV 920 GCCGGC G^CCGGC
PleI 349 GAGTC GAGTC(4/5)
PvuII 1157 CAGCTG CAG^CTG
SacI 224 GAGCTC GAGCT^C
SfaNI 196 GATGC GCATC(5/9)
SmlI 391 CTTGAG C^T[CT][GA]AG
SphI 1322 GCATGC GCATG^C
XmnI 142 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 1807 CCGC CCGC(-3/-1)
25 GCGG
BamHI 594 GGATCC G^GATCC
751 GGATCC
BanI 543 GGTGCC G^G[CT][GA]CC
1599 GGCACC
BbsI 306 GAAGAC GAAGAC(2/6)
1144 GAAGAC
BciVI 57 GGATAC GTATCC(6/5)
1875 GGATAC
BfaI 1265 CTAG C^TAG
1625 CTAG
BpmI 1275 CTCCAG CTGGAG(16/14)
1382 CTCCAG
BpuAI 306 GAAGAC GAAGAC(2/6)
1144 GAAGAC
BsmFI 360 GGGAC GGGAC(10/14)
1608 GGGAC
DraII 360 GGGACCT [GA]G^G[ACGT]CC[CT]
1641 GGGTCCT
DsaI 1665 CCGTGG C^C[GA][CT]GG
1674 CCGTGG
EarI 40 GAAGAG CTCTTC(1/4)
1502 GAAGAG
EcoO109I 360 GGGACCT [GA]G^G[ACGT]CC[CT]
1641 GGGTCCT
EcoT22I 623 ATGCAT ATGCA^T
1127 ATGCAT
HgaI 917 GACGC GACGC(5/10)
1752 GCGTC
Ksp632I 40 GAAGAG CTCTTC(1/4)
1502 GAAGAG
MaeI 1265 CTAG C^TAG
1625 CTAG
MaeII 582 ACGT A^CGT
824 ACGT
MslI 287 CATTTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
720 CATTTCTGTG
NsiI 623 ATGCAT ATGCA^T
1127 ATGCAT
NspI 267 ACATGT [GA]CATG^[CT]
1322 GCATGC
PpuMI 360 GGGACCT [GA]G^G[AT]CC[CT]
1641 GGGTCCT
SfcI 238 CTACAG C^T[GA][CT]AG
312 CTACAG
SspI 144 AATATT AAT^ATT
1065 AATATT
TaqI 173 TCGA T^CGA
1100 TCGA
TfiI 861 GAATC G^A[AT]TC
1744 GATTC
TthHB8I 173 TCGA T^CGA
1100 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbvI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
StuI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII