Restriction map for
pF1KB5334
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MunI MvnI NarI NciI NcoI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 432 AAATTT [GA]^AATT[CT]
AcyI 1410 GACGCC G[GA]^CG[CT]C
Alw44I 105 GTGCAC G^TGCAC
AlwNI 94 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 105 GTGCAC G^TGCAC
ApoI 432 AAATTT [GA]^AATT[CT]
AspHI 105 GTGCAC G[AT]GC[AT]^C
AvaI 49 CCCGAG C^[CT]CG[GA]G
AviII 462 TGCGCA TGC^GCA
AvrII 1291 CCTAGG C^CTAGG
BalI 317 TGGCCA TGG^CCA
BamHI 159 GGATCC G^GATCC
BciVI 939 GTATCC GTATCC(6/5)
BglII 897 AGATCT A^GATCT
BlnI 1291 CCTAGG C^CTAGG
BsaHI 1410 GACGCC G[GA]^CG[CT]C
BsiHKAI 105 GTGCAC G[AT]GC[AT]^C
BsoBI 49 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1184 TGTACA T^GTACA
BsrBI 690 GAGCGG CCGCTC(-3/-3)
BsrFI 1550 GCCGGC [GA]^CCGG[CT]
BsrGI 1184 TGTACA T^GTACA
BstXI 1105 CCACTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1550 GCCGGC [GA]^CCGG[CT]
DraI 922 TTTAAA TTT^AAA
DrdI 1144 GACAATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 715 ATGCAT ATGCA^T
FspI 462 TGCGCA TGC^GCA
HaeII 249 AGCGCC [GA]GCGC^[CT]
Hin1I 1410 GACGCC G[GA]^CG[CT]C
HincII 1251 GTCAAC GT[CT]^[GA]AC
HindII 1251 GTCAAC GT[CT]^[GA]AC
MluNI 317 TGGCCA TGG^CCA
MscI 317 TGGCCA TGG^CCA
MseI 923 TTAA T^TAA
NaeI 1550 GCCGGC GCC^GGC
NgoMIV 1550 GCCGGC G^CCGGC
NsiI 715 ATGCAT ATGCA^T
NspI 713 ACATGC [GA]CATG^[CT]
PflMI 1540 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1467 AGGACCT [GA]G^G[AT]CC[CT]
PstI 706 CTGCAG CTGCA^G
SspBI 1184 TGTACA T^GTACA
TaqI 1140 TCGA T^CGA
Tru9I 923 TTAA T^TAA
Tsp45I 1079 GTGAC ^GT[GC]AC
TthHB8I 1140 TCGA T^CGA
Van91I 1540 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 87 CCTGAGG CC^T[ACGT]AGG
640 CCTCAGG
BanI 45 GGTGCC G^G[CT][GA]CC
646 GGCACC
BsmBI 52 GAGACG CGTCTC(1/5)
1408 GAGACG
BsmFI 212 GGGAC GGGAC(10/14)
96 GTCCC
BsmI 282 GCATTC GAATGC(1/-1)
540 GCATTC
BstAPI 93 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
708 GCAGTACATGC
Bsu36I 87 CCTGAGG CC^T[ACGT]AGG
640 CCTCAGG
Cac8I 80 GCTCGC GC[ACGT]^[ACGT]GC
1550 GCCGGC
DraII 420 AGGGCCT [GA]G^G[ACGT]CC[CT]
1467 AGGACCT
EarI 178 GAAGAG CTCTTC(1/4)
1191 GAAGAG
Eco81I 87 CCTGAGG CC^T[ACGT]AGG
640 CCTCAGG
EcoO109I 420 AGGGCCT [GA]G^G[ACGT]CC[CT]
1467 AGGACCT
HapII 673 CCGG C^CGG
1551 CCGG
HgaI 63 GACGC GACGC(5/10)
1410 GACGC
HpaII 673 CCGG C^CGG
1551 CCGG
HphI 1336 GGTGA GGTGA(8/7)
749 TCACC
Ksp632I 178 GAAGAG CTCTTC(1/4)
1191 GAAGAG
MaeIII 632 GTTAC ^GT[ACGT]AC
1079 GTGAC
MslI 750 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1106 CACTCCTGTG
MspI 673 CCGG C^CGG
1551 CCGG
NdeI 194 CATATG CA^TATG
466 CATATG
PleI 445 GAGTC GAGTC(4/5)
625 GAGTC
SfcI 510 CTGTAG C^T[GA][CT]AG
706 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BpmI BsaI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
MaeI MboI MboII MflI MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI TseI
Tsp509I TspRI XhoII