Restriction map for
pF1KB5298
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI ApoI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BclI BfaI BfrI BlnI BpuAI
BsaHI BseAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BstBI
BstEII BstPI BstXI Bsu36I ClaI
CpoI DraI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Aor51HI 1250 AGCGCT AGC^GCT
AscI 1445 GGCGCGCC GG^CGCGCC
Asp700I 984 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 809 TGGCCA TGG^CCA
BcgI 173 GCAGACACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1516 GGATAC GTATCC(6/5)
BglII 692 AGATCT A^GATCT
BlpI 342 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 342 GCTCAGC GC^T[ACGT]AGC
BsaBI 1341 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 830 ACCGGA [AT]^CCGG[AT]
Bsp1407I 357 TGTACA T^GTACA
BsrFI 467 ACCGGC [GA]^CCGG[CT]
BsrGI 357 TGTACA T^GTACA
BssHII 1446 GCGCGC G^CGCGC
BssSI 703 CACGAG CACGAG(-5/-1)
Bst1107I 1051 GTATAC GTA^TAC
BstAPI 762 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 195 CATCC GGATG(2/0)
BstZ17I 1051 GTATAC GTA^TAC
CelII 342 GCTCAGC GC^T[ACGT]AGC
Cfr10I 467 ACCGGC [GA]^CCGG[CT]
DrdI 1069 GACAACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1356 CGGCCG C^GGCCG
EclXI 1356 CGGCCG C^GGCCG
Eco47III 1250 AGCGCT AGC^GCT
Eco52I 1356 CGGCCG C^GGCCG
EcoT14I 1080 CCAAGG C^C[AT][AT]GG
FokI 195 CATCC GGATG(9/13)
MaeIII 1220 GTGAC ^GT[ACGT]AC
MamI 1341 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 809 TGGCCA TGG^CCA
MscI 809 TGGCCA TGG^CCA
NotI 1355 GCGGCCGC GC^GGCCGC
NspI 1013 GCATGT [GA]CATG^[CT]
PflMI 1268 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1096 GAGTC GAGTC(4/5)
PshAI 1565 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 340 GAGCTC GAGCT^C
SexAI 1535 ACCAGGT A^CC[AT]GGT
SspBI 357 TGTACA T^GTACA
StuI 1752 AGGCCT AGG^CCT
StyI 1080 CCAAGG C^C[AT][AT]GG
TfiI 140 GAATC G^A[AT]TC
Tsp45I 1220 GTGAC ^GT[GC]AC
Tsp509I 402 AATT ^AATT
Van91I 1268 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1745 CCATCTCAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 984 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1531 GTGCAC G^TGCAC
1687 GTGCAC
ApaI 647 GGGCCC GGGCC^C
1726 GGGCCC
ApaLI 1531 GTGCAC G^TGCAC
1687 GTGCAC
BcnI 129 CCGGG CC^[GC]GG
1836 CCCGG
BsaAI 1282 TACGTG [CT]AC^GT[GA]
1684 TACGTG
BsaI 628 GAGACC GGTCTC(1/5)
618 GGTCTC
BsiEI 880 CGGTCG CG[GA][CT]^CG
1356 CGGCCG
BsmI 1239 GAATGC GAATGC(1/-1)
914 GCATTC
BsrBI 1653 CCGCTC CCGCTC(-3/-3)
1782 CCGCTC
BsrDI 1614 CATTGC GCAATG(2/0)
353 GCAATG
EcoNI 669 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1152 CCTGCAGCAGG
HaeII 814 AGCGCC [GA]GCGC^[CT]
1250 AGCGCT
HgaI 1326 GACGC GACGC(5/10)
208 GCGTC
HinfI 140 GAATC G^A[ACGT]TC
1096 GAGTC
KspI 1665 CCGCGG CCGC^GG
1806 CCGCGG
MslI 1029 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1614 CATTGCTGTG
NciI 129 CCGGG CC^[GC]GG
1836 CCCGG
PvuII 1551 CAGCTG CAG^CTG
1732 CAGCTG
SacII 1665 CCGCGG CCGC^GG
1806 CCGCGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AspHI AvaII BanII
BbvCI BbvI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI TspRI TthHB8I XhoII