Restriction map for
pF1KB5274
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AciI AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KpnI KspI MluI
MluNI MscI MseI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 202 GTAGAC GT^[AC][GT]AC
AccIII 443 TCCGGA T^CCGGA
AcsI 839 GAATTC [GA]^AATT[CT]
AcyI 152 GGCGTC G[GA]^CG[CT]C
AlwNI 515 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 839 GAATTC [GA]^AATT[CT]
AspHI 970 GAGCTC G[AT]GC[AT]^C
AvaII 174 GGACC G^G[AT]CC
BanII 970 GAGCTC G[GA]GC[CT]^C
BbvCI 675 CCTCAGC CCTCAGC(-5/-2)
BciVI 286 GTATCC GTATCC(6/5)
BglII 753 AGATCT A^GATCT
BmyI 970 GAGCTC G[AGT]GC[ACT]^C
BsaHI 152 GGCGTC G[GA]^CG[CT]C
BsaWI 443 TCCGGA [AT]^CCGG[AT]
BseAI 443 TCCGGA T^CCGGA
BsiHKAI 970 GAGCTC G[AT]GC[AT]^C
Bsp1286I 970 GAGCTC G[AGT]GC[ACT]^C
BspEI 443 TCCGGA T^CCGGA
BspHI 917 TCATGA T^CATGA
BspMI 206 ACCTGC ACCTGC(4/8)
BstXI 821 CCAATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 399 CCACGG C^C[GA][CT]GG
EcoRI 839 GAATTC G^AATTC
HapII 444 CCGG C^CGG
HgaI 153 GCGTC GACGC(5/10)
Hin1I 152 GGCGTC G[GA]^CG[CT]C
HincII 109 GTCAAC GT[CT]^[GA]AC
HindII 109 GTCAAC GT[CT]^[GA]AC
HindIII 225 AAGCTT A^AGCTT
HpaII 444 CCGG C^CGG
HphI 301 GGTGA GGTGA(8/7)
MaeII 54 ACGT A^CGT
MfeI 131 CAATTG C^AATTG
MroI 443 TCCGGA T^CCGGA
MspI 444 CCGG C^CGG
MunI 131 CAATTG C^AATTG
MwoI 1017 GCAAGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 334 GCTAGC G^CTAGC
PleI 634 GACTC GAGTC(4/5)
RcaI 917 TCATGA T^CATGA
SacI 970 GAGCTC GAGCT^C
ScaI 532 AGTACT AGT^ACT
SfcI 769 CTACAG C^T[GA][CT]AG
StuI 585 AGGCCT AGG^CCT
Tsp45I 617 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 343 GATCC GGATC(4/5)
909 GGATC
BbsI 447 GAAGAC GAAGAC(2/6)
985 GAAGAC
BbvI 680 GCAGC GCAGC(8/12)
683 GCAGC
BpuAI 447 GAAGAC GAAGAC(2/6)
985 GAAGAC
BsaBI 298 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
304 GATCTCTATC
BsaI 703 GAGACC GGTCTC(1/5)
880 GAGACC
BsaJI 399 CCACGG C^C[ACGT][ACGT]GG
884 CCCAGG
BstYI 753 AGATCT [GA]^GATC[CT]
909 GGATCT
Cfr13I 174 GGACC G^G[ACGT]CC
627 GGGCC
EarI 597 CTCTTC CTCTTC(1/4)
432 GAAGAG
EcoRV 268 GATATC GAT^ATC
592 GATATC
Fnu4HI 680 GCAGC GC^[ACGT]GC
683 GCAGC
ItaI 680 GCAGC GC^[ACGT]GC
683 GCAGC
Ksp632I 597 CTCTTC CTCTTC(1/4)
432 GAAGAG
MaeIII 138 GTTAC ^GT[ACGT]AC
617 GTCAC
MamI 298 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
304 GATCTCTATC
MflI 753 AGATCT [GA]^GATC[CT]
909 GGATCT
MspA1I 263 CAGCTG C[AC]G^C[GT]G
826 CAGCTG
PvuII 263 CAGCTG CAG^CTG
826 CAGCTG
Sau96I 174 GGACC G^G[ACGT]CC
627 GGGCC
SmlI 115 CTCAAG C^T[CT][GA]AG
940 CTCAAG
TseI 680 GCAGC G^C[AT]GC
683 GCAGC
TspRI 339 CACTG CA[GC]TG[ACGT][ACGT]^
687 CAGTG
XhoII 753 AGATCT [GA]^GATC[CT]
909 GGATCT
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BfaI BpmI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII FokI HaeIII HinfI MaeI
MboI MboII MnlI MslI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI TfiI Tsp509I