Restriction map for
pF1KB5268
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstEII BstF5I BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeII
MamI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AfaI 867 GTAC GT^AC
AluI 63 AGCT AG^CT
AlwI 54 GATCC GGATC(4/5)
AocI 609 CCTGAGG CC^T[ACGT]AGG
Aor51HI 729 AGCGCT AGC^GCT
AviII 119 TGCGCA TGC^GCA
BalI 2 TGGCCA TGG^CCA
BsaI 993 GAGACC GGTCTC(1/5)
BsmAI 993 GAGAC GTCTC(1/5)
BspMI 173 ACCTGC ACCTGC(4/8)
BsrDI 981 GCAATG GCAATG(2/0)
BsrI 900 ACTGG ACTGG(1/-1)
BssSI 391 CTCGTG CACGAG(-5/-1)
BstAPI 354 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 609 CCTGAGG CC^T[ACGT]AGG
DpnII 54 GATC ^GATC
Eco47III 729 AGCGCT AGC^GCT
Eco81I 609 CCTGAGG CC^T[ACGT]AGG
EcoNI 1009 CCTGCCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 119 TGCGCA TGC^GCA
MaeIII 823 GTCAC ^GT[ACGT]AC
MboI 54 GATC ^GATC
MboII 584 TCTTC GAAGA(8/7)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NdeII 54 GATC ^GATC
PpuMI 634 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 877 CTGCAG CTGCA^G
PvuII 62 CAGCTG CAG^CTG
RsaI 867 GTAC GT^AC
Sau3AI 54 GATC ^GATC
SfaNI 581 GCATC GCATC(5/9)
SfcI 877 CTGCAG C^T[GA][CT]AG
Tsp45I 823 GTCAC ^GT[GC]AC
Tsp509I 746 AATT ^AATT
XcmI 191 CCATCATGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 568 GGCGCC G[GA]^CG[CT]C
786 GGCGCC
Alw44I 352 GTGCAC G^TGCAC
920 GTGCAC
ApaLI 352 GTGCAC G^TGCAC
920 GTGCAC
BbeI 568 GGCGCC GGCGC^C
786 GGCGCC
BpmI 988 CTCCAG CTGGAG(16/14)
1031 CTCCAG
BsaHI 568 GGCGCC G[GA]^CG[CT]C
786 GGCGCC
BsgI 303 CTGCAC GTGCAG(16/14)
945 CTGCAC
BsrFI 297 GCCGGC [GA]^CCGG[CT]
624 GCCGGC
Cfr10I 297 GCCGGC [GA]^CCGG[CT]
624 GCCGGC
Hin1I 568 GGCGCC G[GA]^CG[CT]C
786 GGCGCC
HinfI 641 GACTC G^A[ACGT]TC
652 GACTC
KasI 568 GGCGCC G^GCGCC
786 GGCGCC
MslI 399 CACCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
582 CATCTTCGTG
NaeI 297 GCCGGC GCC^GGC
624 GCCGGC
NarI 568 GGCGCC GG^CGCC
786 GGCGCC
NgoMIV 297 GCCGGC G^CCGGC
624 GCCGGC
NlaIII 195 CATG CATG^
260 CATG
NotI 181 GCGGCCGC GC^GGCCGC
375 GCGGCCGC
PleI 641 GACTC GAGTC(4/5)
652 GACTC
SfoI 568 GGCGCC GGC^GCC
786 GGCGCC
SmaI 226 CCCGGG CCC^GGG
703 CCCGGG
XmaCI 226 CCCGGG C^CCGGG
703 CCCGGG
XmaI 226 CCCGGG C^CCGGG
703 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI KspI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV SacII Sau96I ScrFI
SfiI TseI TspRI