Restriction map for
pF1KB5212
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AvrII BbeI BbrPI
BbsI BbvCI BciVI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FseI
HaeII HindIII HpaI KasI KpnI
KspI MaeI MfeI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StuI SwaI Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 106 GTCTAC GT^[AC][GT]AC
AcyI 1064 GGCGTC G[GA]^CG[CT]C
AlwNI 583 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 961 CCTGAGG CC^T[ACGT]AGG
AspI 643 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 845 TGCGCA TGC^GCA
BanI 339 GGTGCC G^G[CT][GA]CC
BanII 979 GAGCCC G[GA]GC[CT]^C
BcgI 717 CGATGTGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 551 TGATCA T^GATCA
BglII 882 AGATCT A^GATCT
BpmI 143 CTGGAG CTGGAG(16/14)
BsaBI 177 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1064 GGCGTC G[GA]^CG[CT]C
BsaI 625 GAGACC GGTCTC(1/5)
BseRI 43 GAGGAG GAGGAG(10/8)
BsgI 274 GTGCAG GTGCAG(16/14)
BsmAI 625 GAGAC GTCTC(1/5)
BspLU11I 659 ACATGT A^CATGT
Bsu36I 961 CCTGAGG CC^T[ACGT]AGG
DrdI 97 GACAAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 347 CCGTGG C^C[GA][CT]GG
Eco81I 961 CCTGAGG CC^T[ACGT]AGG
EcoRI 433 GAATTC G^AATTC
EcoT14I 449 CCAAGG C^C[AT][AT]GG
FauI 228 CCCGC CCCGC(4/6)
FbaI 551 TGATCA T^GATCA
FspI 845 TGCGCA TGC^GCA
HgaI 1065 GCGTC GACGC(5/10)
Hin1I 1064 GGCGTC G[GA]^CG[CT]C
HincII 649 GTCAAC GT[CT]^[GA]AC
HindII 649 GTCAAC GT[CT]^[GA]AC
MamI 177 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 996 ACGCGT A^CGCGT
PflFI 643 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 795 CCTGCAGG CCTGCA^GG
SexAI 335 ACCTGGT A^CC[AT]GGT
SmaI 1004 CCCGGG CCC^GGG
Sse8387I 795 CCTGCAGG CCTGCA^GG
StyI 449 CCAAGG C^C[AT][AT]GG
Tth111I 643 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1004 CCCGGG C^CCGGG
XmaI 1004 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 682 CGCG CG^CG
997 CGCG
AcsI 433 GAATTC [GA]^AATT[CT]
940 GAATTT
AflIII 659 ACATGT A^C[GA][CT]GT
996 ACGCGT
ApoI 433 GAATTC [GA]^AATT[CT]
940 GAATTT
Asp700I 369 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
810 GAACCTCTTC
BamHI 182 GGATCC G^GATCC
701 GGATCC
BstAPI 120 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
879 GCAAGATCTGC
BstUI 682 CGCG CG^CG
997 CGCG
EarI 814 CTCTTC CTCTTC(1/4)
220 GAAGAG
EcoNI 294 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
795 CCTGCAGGAGG
HinfI 148 GAATC G^A[ACGT]TC
617 GAATC
HphI 256 GGTGA GGTGA(8/7)
1088 TCACC
Ksp632I 814 CTCTTC CTCTTC(1/4)
220 GAAGAG
MseI 194 TTAA T^TAA
203 TTAA
MspA1I 85 CAGCTG C[AC]G^C[GT]G
508 CAGCTG
MvnI 682 CGCG CG^CG
997 CGCG
PstI 88 CTGCAG CTGCA^G
796 CTGCAG
PvuII 85 CAGCTG CAG^CTG
508 CAGCTG
ScaI 527 AGTACT AGT^ACT
1013 AGTACT
SfaNI 967 GATGC GCATC(5/9)
824 GCATC
SfcI 88 CTGCAG C^T[GA][CT]AG
796 CTGCAG
SphI 444 GCATGC GCATG^C
1154 GCATGC
TfiI 148 GAATC G^A[AT]TC
617 GAATC
Tru9I 194 TTAA T^TAA
203 TTAA
Tsp509I 434 AATT ^AATT
941 AATT
XmnI 369 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
810 GAACCTCTTC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BbvI BcnI BmyI
BsaJI BsiYI BslI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI RsaI Sau3AI Sau96I ScrFI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII