Restriction map for
pF1KB5182
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 949 GAATTC [GA]^AATT[CT]
AlwNI 1084 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 949 GAATTC [GA]^AATT[CT]
Asp700I 639 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 125 TGGCCA TGG^CCA
BanI 574 GGCACC G^G[CT][GA]CC
BclI 749 TGATCA T^GATCA
BglI 229 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1387 GAGACC GGTCTC(1/5)
BsgI 47 CTGCAC GTGCAG(16/14)
BsiEI 1097 CGGCCG CG[GA][CT]^CG
BstAPI 492 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 977 TTCGAA TT^CGAA
BstXI 107 CCATCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 1025 CTCAG C^T[ACGT]AG
DraIII 50 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1271 CCGTGG C^C[GA][CT]GG
EagI 1097 CGGCCG C^GGCCG
EclXI 1097 CGGCCG C^GGCCG
Eco52I 1097 CGGCCG C^GGCCG
EcoRI 949 GAATTC G^AATTC
FbaI 749 TGATCA T^GATCA
MluNI 125 TGGCCA TGG^CCA
MscI 125 TGGCCA TGG^CCA
MseI 928 TTAA T^TAA
NspV 977 TTCGAA TT^CGAA
PleI 1126 GAGTC GAGTC(4/5)
PshAI 813 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 44 CAGCTG CAG^CTG
SapI 1221 GAAGAGC GCTCTTC(1/4)
SfuI 977 TTCGAA TT^CGAA
SmaI 519 CCCGGG CCC^GGG
SmlI 1285 CTCAAG C^T[CT][GA]AG
TaqI 978 TCGA T^CGA
Tru9I 928 TTAA T^TAA
TthHB8I 978 TCGA T^CGA
XmaCI 519 CCCGGG C^CCGGG
XmaI 519 CCCGGG C^CCGGG
XmnI 639 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 179 ACATGT A^C[GA][CT]GT
1058 ACATGT
AlwI 711 GATCC GGATC(4/5)
891 GATCC
AspHI 913 GAGCAC G[AT]GC[AT]^C
1149 GTGCTC
BbsI 789 GAAGAC GAAGAC(2/6)
810 GAAGAC
BciVI 514 GGATAC GTATCC(6/5)
315 GTATCC
BglII 756 AGATCT A^GATCT
1496 AGATCT
BpuAI 789 GAAGAC GAAGAC(2/6)
810 GAAGAC
BsaBI 885 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1362 GATTCGCATC
BsaWI 1352 ACCGGA [AT]^CCGG[AT]
1478 ACCGGA
BsiHKAI 913 GAGCAC G[AT]GC[AT]^C
1149 GTGCTC
BspLU11I 179 ACATGT A^CATGT
1058 ACATGT
BsrDI 908 GCAATG GCAATG(2/0)
1508 GCAATG
EarI 967 GAAGAG CTCTTC(1/4)
1221 GAAGAG
HphI 1275 GGTGA GGTGA(8/7)
536 TCACC
Ksp632I 967 GAAGAG CTCTTC(1/4)
1221 GAAGAG
MaeII 122 ACGT A^CGT
1052 ACGT
MamI 885 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1362 GATTCGCATC
MslI 1047 CACAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1368 CATCATGGTG
PflMI 52 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
475 CCACTCCCTGG
PpuMI 620 AGGTCCT [GA]G^G[AT]CC[CT]
1247 GGGACCT
SexAI 51 ACCAGGT A^CC[AT]GGT
1169 ACCTGGT
SphI 98 GCATGC GCATG^C
1262 GCATGC
TfiI 1362 GATTC G^A[AT]TC
1485 GATTC
Tsp509I 926 AATT ^AATT
950 AATT
Van91I 52 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
475 CCACTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DpnII DraII EaeI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI XhoII