Restriction map for
pF1KB5162
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BpuAI BsaAI BsaHI BsaI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI TspRI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwI 502 GATCC GGATC(4/5)
Aor51HI 268 AGCGCT AGC^GCT
Asp700I 348 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 328 GTGCTC G[AT]GC[AT]^C
BanI 459 GGTGCC G^G[CT][GA]CC
BclI 96 TGATCA T^GATCA
BglII 406 AGATCT A^GATCT
BmyI 328 GTGCTC G[AGT]GC[ACT]^C
BpmI 420 CTCCAG CTGGAG(16/14)
BsaBI 407 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 641 ACCGGA [AT]^CCGG[AT]
BsgI 313 GTGCAG GTGCAG(16/14)
BsiHKAI 328 GTGCTC G[AT]GC[AT]^C
BsmAI 418 GTCTC GTCTC(1/5)
Bsp1286I 328 GTGCTC G[AGT]GC[ACT]^C
BsrFI 604 ACCGGC [GA]^CCGG[CT]
BstYI 406 AGATCT [GA]^GATC[CT]
Cac8I 589 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 269 GCGC GCG^C
Cfr10I 604 ACCGGC [GA]^CCGG[CT]
Eco47III 268 AGCGCT AGC^GCT
EcoT14I 203 CCAAGG C^C[AT][AT]GG
FbaI 96 TGATCA T^GATCA
HaeII 268 AGCGCT [GA]GCGC^[CT]
HhaI 269 GCGC GCG^C
HinP1I 269 GCGC G^CGC
MaeIII 628 GTCAC ^GT[ACGT]AC
MamI 407 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 406 AGATCT [GA]^GATC[CT]
MseI 122 TTAA T^TAA
MspA1I 229 CAGCGG C[AC]G^C[GT]G
MwoI 462 GCCACAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 608 GCATGT [GA]CATG^[CT]
PshAI 474 GACATACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 35 CTGCAG CTGCA^G
ScaI 350 AGTACT AGT^ACT
SfcI 35 CTGCAG C^T[GA][CT]AG
SmlI 73 CTCAAG C^T[CT][GA]AG
StyI 203 CCAAGG C^C[AT][AT]GG
Tru9I 122 TTAA T^TAA
Tsp45I 628 GTCAC ^GT[GC]AC
XhoII 406 AGATCT [GA]^GATC[CT]
XmnI 348 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 108 GTAC GT^AC
351 GTAC
BbvI 315 GCAGC GCAGC(8/12)
468 GCAGC
BcnI 344 CCGGG CC^[GC]GG
534 CCGGG
BlpI 48 GCTGAGC GC^T[ACGT]AGC
219 GCTTAGC
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
219 GCTTAGC
BsrI 104 ACTGG ACTGG(1/-1)
575 ACTGG
BstXI 169 CCACATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
507 CCAGGAGGATGG
CelII 48 GCTGAGC GC^T[ACGT]AGC
219 GCTTAGC
Cfr13I 242 GGGCC G^G[ACGT]CC
486 GGCCC
DraII 241 GGGGCCT [GA]G^G[ACGT]CC[CT]
485 AGGCCCT
DsaI 175 CCGTGG C^C[GA][CT]GG
594 CCACGG
EcoO109I 241 GGGGCCT [GA]G^G[ACGT]CC[CT]
485 AGGCCCT
FauI 617 CCCGC CCCGC(4/6)
444 GCGGG
HaeIII 243 GGCC GG^CC
486 GGCC
HphI 366 GGTGA GGTGA(8/7)
629 TCACC
MaeII 479 ACGT A^CGT
647 ACGT
MboII 88 GAAGA GAAGA(8/7)
211 GAAGA
NciI 344 CCGGG CC^[GC]GG
534 CCGGG
PflMI 549 CCATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
572 CCAACTGGTGG
RsaI 108 GTAC GT^AC
351 GTAC
Sau96I 242 GGGCC G^G[ACGT]CC
486 GGCCC
TseI 315 GCAGC G^C[AT]GC
468 GCAGC
Tsp509I 376 AATT ^AATT
622 AATT
Van91I 549 CCATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
572 CCAACTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsmFI BssKI BstF5I BstNI DdeI
DpnII EcoRII Fnu4HI FokI HapII
HpaII ItaI MboI MnlI MslI
MspI MvaI NdeII NlaIII NlaIV
Sau3AI ScrFI