Restriction map for
pF1KB5152
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MflI MluI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tsp509I
Tth111I XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1386 CGCG CG^CG
AccIII 518 TCCGGA T^CCGGA
AflIII 1520 ACACGT A^C[GA][CT]GT
AlwI 1026 GGATC GGATC(4/5)
AocI 1277 CCTCAGG CC^T[ACGT]AGG
BbrPI 1204 CACGTG CAC^GTG
BcgI 1003 GCACTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 518 TCCGGA [AT]^CCGG[AT]
BseAI 518 TCCGGA T^CCGGA
BsiEI 1475 CGGCCG CG[GA][CT]^CG
BsiWI 899 CGTACG C^GTACG
BspEI 518 TCCGGA T^CCGGA
BspMI 1462 GCAGGT ACCTGC(4/8)
BssSI 811 CACGAG CACGAG(-5/-1)
BstUI 1386 CGCG CG^CG
Bsu36I 1277 CCTCAGG CC^T[ACGT]AGG
DraIII 1219 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1475 CGGCCG C^GGCCG
EclXI 1475 CGGCCG C^GGCCG
Eco52I 1475 CGGCCG C^GGCCG
Eco81I 1277 CCTCAGG CC^T[ACGT]AGG
FseI 103 GGCCGGCC GGCCGG^CC
MroI 518 TCCGGA T^CCGGA
MseI 1706 TTAA T^TAA
MslI 1200 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1386 CGCG CG^CG
NotI 1474 GCGGCCGC GC^GGCCGC
PaeR7I 1109 CTCGAG C^TCGAG
PmaCI 1204 CACGTG CAC^GTG
PmlI 1204 CACGTG CAC^GTG
PshAI 1147 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1165 CTGCAG CTGCA^G
ScaI 443 AGTACT AGT^ACT
SfiI 1566 GGCCCCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 1833 GAATC G^A[AT]TC
Tru9I 1706 TTAA T^TAA
XhoI 1109 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 1081 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1526 CAGCGGCTG
BalI 1260 TGGCCA TGG^CCA
1655 TGGCCA
BbeI 21 GGCGCC GGCGC^C
316 GGCGCC
BbsI 1180 GAAGAC GAAGAC(2/6)
536 GTCTTC
BbvCI 668 CCTCAGC CCTCAGC(-5/-2)
881 CCTCAGC
BfaI 496 CTAG C^TAG
1453 CTAG
BpmI 912 CTCCAG CTGGAG(16/14)
681 CTGGAG
BpuAI 1180 GAAGAC GAAGAC(2/6)
536 GTCTTC
BsaI 1892 GAGACC GGTCTC(1/5)
1153 GGTCTC
BsmBI 909 CGTCTC CGTCTC(1/5)
338 GAGACG
BsrI 1005 ACTGG ACTGG(1/-1)
441 CCAGT
BstAPI 1245 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1809 GCAGCAAGTGC
EarI 264 CTCTTC CTCTTC(1/4)
1864 GAAGAG
HgaI 340 GACGC GACGC(5/10)
908 GCGTC
KasI 21 GGCGCC G^GCGCC
316 GGCGCC
Ksp632I 264 CTCTTC CTCTTC(1/4)
1864 GAAGAG
MaeI 496 CTAG C^TAG
1453 CTAG
MluNI 1260 TGGCCA TGG^CCA
1655 TGGCCA
MscI 1260 TGGCCA TGG^CCA
1655 TGGCCA
NaeI 104 GCCGGC GCC^GGC
1234 GCCGGC
NarI 21 GGCGCC GG^CGCC
316 GGCGCC
NgoMIV 104 GCCGGC G^CCGGC
1234 GCCGGC
NspI 1241 ACATGC [GA]CATG^[CT]
1721 GCATGT
PflMI 1080 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1218 CCACGCCGTGG
PpuMI 433 GGGACCT [GA]G^G[AT]CC[CT]
524 AGGTCCC
PvuII 829 CAGCTG CAG^CTG
971 CAGCTG
SacI 43 GAGCTC GAGCT^C
676 GAGCTC
SfcI 1165 CTGCAG C^T[GA][CT]AG
1443 CTACAG
SfoI 21 GGCGCC GGC^GCC
316 GGCGCC
TaqI 1012 TCGA T^CGA
1110 TCGA
TthHB8I 1012 TCGA T^CGA
1110 TCGA
Van91I 1080 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1218 CCACGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcyI AfaI AluI ApaI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI StuI TseI
Tsp45I TspRI XmaCI XmaI