Restriction map for
pF1KB5140
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DrdI Eam1105I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MaeI MaeIII MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 883 GTAGAC GT^[AC][GT]AC
Aor51HI 65 AGCGCT AGC^GCT
Asp700I 447 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 514 GTCTTC GAAGAC(2/6)
BglII 479 AGATCT A^GATCT
BpuAI 514 GTCTTC GAAGAC(2/6)
BsaAI 70 TACGTG [CT]AC^GT[GA]
BsaBI 1036 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 800 GGGAC GGGAC(10/14)
BspMI 161 ACCTGC ACCTGC(4/8)
BsrBI 630 CCGCTC CCGCTC(-3/-3)
BssHII 498 GCGCGC G^CGCGC
BstAPI 1015 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 479 AGATCT [GA]^GATC[CT]
Eco47III 65 AGCGCT AGC^GCT
HincII 301 GTCAAC GT[CT]^[GA]AC
HindII 301 GTCAAC GT[CT]^[GA]AC
MamI 1036 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 479 AGATCT [GA]^GATC[CT]
NaeI 1064 GCCGGC GCC^GGC
NcoI 440 CCATGG C^CATGG
NgoMIV 1064 GCCGGC G^CCGGC
NotI 56 GCGGCCGC GC^GGCCGC
SacI 870 GAGCTC GAGCT^C
SapI 216 GCTCTTC GCTCTTC(1/4)
SexAI 878 ACCTGGT A^CC[AT]GGT
SmaI 28 CCCGGG CCC^GGG
StuI 605 AGGCCT AGG^CCT
XhoII 479 AGATCT [GA]^GATC[CT]
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
XmnI 447 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 640 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
1016 CAGAGGCTG
BanII 435 GGGCTC G[GA]GC[CT]^C
870 GAGCTC
BbvCI 174 CCTCAGC CCTCAGC(-5/-2)
681 CCTCAGC
BcnI 28 CCCGG CC^[GC]GG
29 CCGGG
BglI 42 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
395 GCCCCGAGGGC
BsaI 346 GAGACC GGTCTC(1/5)
352 GAGACC
BsmBI 723 CGTCTC CGTCTC(1/5)
704 GAGACG
BsrFI 86 GCCGGT [GA]^CCGG[CT]
1064 GCCGGC
BsrI 1049 ACTGG ACTGG(1/-1)
390 CCAGT
BssSI 745 CACGAG CACGAG(-5/-1)
967 CACGAG
BstXI 149 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1061 CCAGCCGGCTGG
Cfr10I 86 GCCGGT [GA]^CCGG[CT]
1064 GCCGGC
DdeI 175 CTCAG C^T[ACGT]AG
682 CTCAG
EagI 57 CGGCCG C^GGCCG
252 CGGCCG
EclXI 57 CGGCCG C^GGCCG
252 CGGCCG
Eco52I 57 CGGCCG C^GGCCG
252 CGGCCG
EcoT14I 440 CCATGG C^C[AT][AT]GG
1172 CCAAGG
HinfI 924 GACTC G^A[ACGT]TC
1168 GAGTC
NciI 28 CCCGG CC^[GC]GG
29 CCGGG
NspI 467 GCATGT [GA]CATG^[CT]
856 ACATGC
PflMI 230 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
639 CCAGACGCTGG
PleI 924 GACTC GAGTC(4/5)
1168 GAGTC
PstI 163 CTGCAG CTGCA^G
421 CTGCAG
SfaNI 1036 GATGC GCATC(5/9)
404 GCATC
SfcI 163 CTGCAG C^T[GA][CT]AG
421 CTGCAG
StyI 440 CCATGG C^C[AT][AT]GG
1172 CCAAGG
Van91I 230 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
639 CCAGACGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BbeI BbvI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraIII DsaI EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
Ksp632I KspI MaeII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NdeII NlaIII
NlaIV PaeR7I SacII Sau3AI Sau96I
ScrFI SfoI SmlI TaqI TseI
TspRI TthHB8I XhoI