Restriction map for
pF1KB5128
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI BamHI BciVI BfrI
BglII BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoNI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI KpnI MamI MluI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SgrAI
SnaBI SpeI SphI SspBI SwaI
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AatII 1324 GACGTC GACGT^C
AccI 2059 GTCTAC GT^[AC][GT]AC
AccIII 1120 TCCGGA T^CCGGA
Alw44I 724 GTGCAC G^TGCAC
AlwNI 745 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1237 AGCGCT AGC^GCT
ApaI 2184 GGGCCC GGGCC^C
ApaLI 724 GTGCAC G^TGCAC
AspI 2226 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1868 TGCGCA TGC^GCA
AvrII 658 CCTAGG C^CTAGG
BalI 140 TGGCCA TGG^CCA
BbeI 1591 GGCGCC GGCGC^C
BbrPI 1972 CACGTG CAC^GTG
BbsI 494 GTCTTC GAAGAC(2/6)
BbvCI 466 GCTGAGG CCTCAGC(-5/-2)
BcgI 222 GCAGCTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 658 CCTAGG C^CTAGG
BlpI 1884 GCTGAGC GC^T[ACGT]AGC
BpmI 239 CTGGAG CTGGAG(16/14)
Bpu1102I 1884 GCTGAGC GC^T[ACGT]AGC
BpuAI 494 GTCTTC GAAGAC(2/6)
BsaAI 1972 CACGTG [CT]AC^GT[GA]
BsaWI 1120 TCCGGA [AT]^CCGG[AT]
BseAI 1120 TCCGGA T^CCGGA
BsiEI 1733 CGGCCG CG[GA][CT]^CG
BsmBI 1081 GAGACG CGTCTC(1/5)
BspEI 1120 TCCGGA T^CCGGA
BsrBI 117 GAGCGG CCGCTC(-3/-3)
BsrDI 488 GCAATG GCAATG(2/0)
BsrFI 333 GCCGGT [GA]^CCGG[CT]
BstAPI 213 GCAGCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 147 GGATCT [GA]^GATC[CT]
CelII 1884 GCTGAGC GC^T[ACGT]AGC
Cfr10I 333 GCCGGT [GA]^CCGG[CT]
EagI 1733 CGGCCG C^GGCCG
EclXI 1733 CGGCCG C^GGCCG
Eco47III 1237 AGCGCT AGC^GCT
Eco52I 1733 CGGCCG C^GGCCG
FspI 1868 TGCGCA TGC^GCA
HgaI 1897 GACGC GACGC(5/10)
KasI 1591 GGCGCC G^GCGCC
KspI 1602 CCGCGG CCGC^GG
MfeI 776 CAATTG C^AATTG
MflI 147 GGATCT [GA]^GATC[CT]
MluNI 140 TGGCCA TGG^CCA
MroI 1120 TCCGGA T^CCGGA
MscI 140 TGGCCA TGG^CCA
MunI 776 CAATTG C^AATTG
NarI 1591 GGCGCC GG^CGCC
NdeI 734 CATATG CA^TATG
PaeR7I 79 CTCGAG C^TCGAG
PflFI 2226 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1972 CACGTG CAC^GTG
PmlI 1972 CACGTG CAC^GTG
PshAI 1083 GACGGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 277 GAGCTC GAGCT^C
SacII 1602 CCGCGG CCGC^GG
SbfI 1566 CCTGCAGG CCTGCA^GG
SfoI 1591 GGCGCC GGC^GCC
Sse8387I 1566 CCTGCAGG CCTGCA^GG
StuI 972 AGGCCT AGG^CCT
Tth111I 2226 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 79 CTCGAG C^TCGAG
XhoII 147 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1324 GACGTC G[GA]^CG[CT]C
1591 GGCGCC
AocI 1542 CCTCAGG CC^T[ACGT]AGG
2017 CCTGAGG
BanII 277 GAGCTC G[GA]GC[CT]^C
2184 GGGCCC
BclI 378 TGATCA T^GATCA
689 TGATCA
BglI 133 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1564 GCCCTGCAGGC
BsaHI 1324 GACGTC G[GA]^CG[CT]C
1591 GGCGCC
BssSI 631 CTCGTG CACGAG(-5/-1)
1915 CTCGTG
BstBI 680 TTCGAA TT^CGAA
922 TTCGAA
BstXI 143 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1757 CCAAGGTGGTGG
Bsu36I 1542 CCTCAGG CC^T[ACGT]AGG
2017 CCTGAGG
DsaI 1602 CCGCGG C^C[GA][CT]GG
1823 CCACGG
Eco81I 1542 CCTCAGG CC^T[ACGT]AGG
2017 CCTGAGG
EcoRI 17 GAATTC G^AATTC
1103 GAATTC
FbaI 378 TGATCA T^GATCA
689 TGATCA
Hin1I 1324 GACGTC G[GA]^CG[CT]C
1591 GGCGCC
NspV 680 TTCGAA TT^CGAA
922 TTCGAA
PstI 1567 CTGCAG CTGCA^G
1690 CTGCAG
PvuII 223 CAGCTG CAG^CTG
1810 CAGCTG
SapI 274 GAAGAGC GCTCTTC(1/4)
2094 GCTCTTC
SexAI 1748 ACCTGGT A^CC[AT]GGT
2063 ACCTGGT
SfuI 680 TTCGAA TT^CGAA
922 TTCGAA
SmaI 188 CCCGGG CCC^GGG
1727 CCCGGG
SmlI 79 CTCGAG C^T[CT][GA]AG
1816 CTCAAG
SspI 390 AATATT AAT^ATT
2304 AATATT
TfiI 683 GAATC G^A[AT]TC
1117 GATTC
XmaCI 188 CCCGGG C^CCGGG
1727 CCCGGG
XmaI 188 CCCGGG C^CCGGG
1727 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BbvI BcnI BfaI BmyI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I