Restriction map for
pF1KB5106
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvrII BamHI BbeI BbvCI BciVI
BfrI BglII BlnI BsaBI BsmBI
Bsp1407I BsrBI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI HaeII HindIII HpaI
KasI MamI MseI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshBI
PvuI RsrII SacI SbfI SexAI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 665 GGTACC G^GTACC
AccI 592 GTCGAC GT^[AC][GT]AC
AccIII 410 TCCGGA T^CCGGA
AclI 2176 AACGTT AA^CGTT
AcsI 2128 AAATTT [GA]^AATT[CT]
AcyI 595 GACGCC G[GA]^CG[CT]C
ApaI 20 GGGCCC GGGCC^C
ApoI 2128 AAATTT [GA]^AATT[CT]
Asp718I 665 GGTACC G^GTACC
BbsI 1878 GAAGAC GAAGAC(2/6)
BcgI 71 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1000 CTAG C^TAG
BlpI 1289 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1289 GCTCAGC GC^T[ACGT]AGC
BpuAI 1878 GAAGAC GAAGAC(2/6)
BsaHI 595 GACGCC G[GA]^CG[CT]C
BsaI 1741 GAGACC GGTCTC(1/5)
BsaWI 410 TCCGGA [AT]^CCGG[AT]
BseAI 410 TCCGGA T^CCGGA
BsiWI 1284 CGTACG C^GTACG
BsmI 946 GCATTC GAATGC(1/-1)
BspDI 460 ATCGAT AT^CGAT
BspEI 410 TCCGGA T^CCGGA
BspLU11I 2331 ACATGT A^CATGT
BsrDI 1308 CATTGC GCAATG(2/0)
BssSI 1573 CACGAG CACGAG(-5/-1)
BstBI 1816 TTCGAA TT^CGAA
CelII 1289 GCTCAGC GC^T[ACGT]AGC
ClaI 460 ATCGAT AT^CGAT
DraIII 1051 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 595 GACGCC G[GA]^CG[CT]C
HincII 592 GTCGAC GT[CT]^[GA]AC
HindII 592 GTCGAC GT[CT]^[GA]AC
KpnI 665 GGTACC GGTAC^C
KspI 1262 CCGCGG CCGC^GG
MaeI 1000 CTAG C^TAG
MluI 1440 ACGCGT A^CGCGT
MroI 410 TCCGGA T^CCGGA
NaeI 67 GCCGGC GCC^GGC
NgoMIV 67 GCCGGC G^CCGGC
NspV 1816 TTCGAA TT^CGAA
PflMI 192 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1389 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2176 AACGTT AA^CGTT
SacII 1262 CCGCGG CCGC^GG
SalI 592 GTCGAC G^TCGAC
SapI 1819 GAAGAGC GCTCTTC(1/4)
ScaI 1457 AGTACT AGT^ACT
SfiI 66 GGCCGGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1816 TTCGAA TT^CGAA
SspI 799 AATATT AAT^ATT
Van91I 192 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AviII 140 TGCGCA TGC^GCA
1484 TGCGCA
BbrPI 899 CACGTG CAC^GTG
919 CACGTG
BclI 1209 TGATCA T^GATCA
1511 TGATCA
BglI 67 GCCGGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1412 GCCAAGGAGGC
BsmAI 1741 GAGAC GTCTC(1/5)
244 GTCTC
BspHI 602 TCATGA T^CATGA
2270 TCATGA
BspMI 338 ACCTGC ACCTGC(4/8)
1684 ACCTGC
BsrFI 67 GCCGGC [GA]^CCGG[CT]
1408 ACCGGC
BsrI 233 ACTGG ACTGG(1/-1)
704 ACTGG
Cfr10I 67 GCCGGC [GA]^CCGG[CT]
1408 ACCGGC
EagI 1280 CGGCCG C^GGCCG
1790 CGGCCG
EclXI 1280 CGGCCG C^GGCCG
1790 CGGCCG
Eco52I 1280 CGGCCG C^GGCCG
1790 CGGCCG
FbaI 1209 TGATCA T^GATCA
1511 TGATCA
FspI 140 TGCGCA TGC^GCA
1484 TGCGCA
MfeI 824 CAATTG C^AATTG
1227 CAATTG
MunI 824 CAATTG C^AATTG
1227 CAATTG
NcoI 1592 CCATGG C^CATGG
1745 CCATGG
NspI 2012 ACATGC [GA]CATG^[CT]
2331 ACATGT
PmaCI 899 CACGTG CAC^GTG
919 CACGTG
PmlI 899 CACGTG CAC^GTG
919 CACGTG
PpuMI 628 AGGACCT [GA]G^G[AT]CC[CT]
934 GGGACCC
RcaI 602 TCATGA T^CATGA
2270 TCATGA
XcmI 730 CCAGAGGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2147 CCATTTGTGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI