Restriction map for
pF1KB5101
##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII BbrPI BfrI BsaAI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII BssSI
BstAPI BstBI BstXI ClaI CpoI
DraI EarI Eco47III EcoT22I FseI
FspI HincII HindII HpaI KpnI
Ksp632I KspI MfeI MluI MroI
MunI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1000 GACGTC GACGT^C
AccII 135 CGCG CG^CG
AflIII 1602 ACACGT A^C[GA][CT]GT
AhdI 2468 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 321 GTGCAC G^TGCAC
ApaLI 321 GTGCAC G^TGCAC
AspEI 2468 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 214 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2646 GGATCC G^GATCC
BbvCI 2301 CCTCAGC CCTCAGC(-5/-2)
BciVI 817 GGATAC GTATCC(6/5)
BclI 749 TGATCA T^GATCA
BsiEI 202 CGGCCG CG[GA][CT]^CG
BsmI 2013 GAATGC GAATGC(1/-1)
Bst1107I 1456 GTATAC GTA^TAC
BstUI 135 CGCG CG^CG
BstZ17I 1456 GTATAC GTA^TAC
DrdI 12 GACGCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 202 CGGCCG C^GGCCG
Eam1105I 2468 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 202 CGGCCG C^GGCCG
Eco52I 202 CGGCCG C^GGCCG
EcoRV 1409 GATATC GAT^ATC
FbaI 749 TGATCA T^GATCA
HindIII 3064 AAGCTT A^AGCTT
MvnI 135 CGCG CG^CG
NaeI 1117 GCCGGC GCC^GGC
NdeI 3092 CATATG CA^TATG
NgoMIV 1117 GCCGGC G^CCGGC
NspI 463 GCATGC [GA]CATG^[CT]
PaeR7I 1739 CTCGAG C^TCGAG
PflFI 214 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 755 ACCTGGT A^CC[AT]GGT
SmaI 165 CCCGGG CCC^GGG
SphI 463 GCATGC GCATG^C
Tth111I 214 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1739 CTCGAG C^TCGAG
XmaCI 165 CCCGGG C^CCGGG
XmaI 165 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AvrII 813 CCTAGG C^CTAGG
3036 CCTAGG
BbeI 59 GGCGCC GGCGC^C
2059 GGCGCC
BbsI 859 GAAGAC GAAGAC(2/6)
1939 GAAGAC
BcgI 1741 CGAGAGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
148 GCAGTACTCTCG
BglII 1763 AGATCT A^GATCT
1919 AGATCT
BlnI 813 CCTAGG C^CTAGG
3036 CCTAGG
BlpI 2071 GCTGAGC GC^T[ACGT]AGC
3115 GCTCAGC
Bpu1102I 2071 GCTGAGC GC^T[ACGT]AGC
3115 GCTCAGC
BpuAI 859 GAAGAC GAAGAC(2/6)
1939 GAAGAC
Bsp1407I 476 TGTACA T^GTACA
2879 TGTACA
BsrBI 576 CCGCTC CCGCTC(-3/-3)
1528 GAGCGG
BsrDI 1403 GCAATG GCAATG(2/0)
2958 GCAATG
BsrFI 200 ACCGGC [GA]^CCGG[CT]
1117 GCCGGC
BsrGI 476 TGTACA T^GTACA
2879 TGTACA
BstEII 2082 GGTCACC G^GT[ACGT]ACC
2373 GGTCACC
BstPI 2082 GGTCACC G^GT[ACGT]ACC
2373 GGTCACC
CelII 2071 GCTGAGC GC^T[ACGT]AGC
3115 GCTCAGC
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
1117 GCCGGC
DraIII 31 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
315 CACACAGTG
EcoNI 756 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1203 CCTGGACCAGG
EcoO65I 2082 GGTCACC G^GT[ACGT]ACC
2373 GGTCACC
EcoRI 554 GAATTC G^AATTC
838 GAATTC
HaeII 59 GGCGCC [GA]GCGC^[CT]
2059 GGCGCC
KasI 59 GGCGCC G^GCGCC
2059 GGCGCC
MseI 917 TTAA T^TAA
2624 TTAA
NarI 59 GGCGCC GG^CGCC
2059 GGCGCC
NcoI 444 CCATGG C^CATGG
3104 CCATGG
SacI 2491 GAGCTC GAGCT^C
2764 GAGCTC
ScaI 150 AGTACT AGT^ACT
2105 AGTACT
SfoI 59 GGCGCC GGC^GCC
2059 GGCGCC
SmlI 1739 CTCGAG C^T[CT][GA]AG
3121 CTCAAG
SspBI 476 TGTACA T^GTACA
2879 TGTACA
Tru9I 917 TTAA T^TAA
2624 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII