Restriction map for
pF1KB5091
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AcyI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaHI BsaI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MfeI MluI MluNI MscI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 1756 TCCGGA T^CCGGA
Alw44I 220 GTGCAC G^TGCAC
ApaLI 220 GTGCAC G^TGCAC
AvaII 30 GGTCC G^G[AT]CC
BanI 840 GGTGCC G^G[CT][GA]CC
BcnI 134 CCCGG CC^[GC]GG
BlpI 39 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BsaBI 1768 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1756 TCCGGA T^CCGGA
BseRI 785 CTCCTC GAGGAG(10/8)
BsmI 553 GCATTC GAATGC(1/-1)
BspDI 1450 ATCGAT AT^CGAT
BspEI 1756 TCCGGA T^CCGGA
BspHI 1149 TCATGA T^CATGA
BsrBI 1752 CCGCTC CCGCTC(-3/-3)
BstAPI 553 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 281 TTCGAA TT^CGAA
BstXI 620 CCAACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1707 AGATCC [GA]^GATC[CT]
CelII 39 GCTCAGC GC^T[ACGT]AGC
ClaI 1450 ATCGAT AT^CGAT
EaeI 1495 CGGCCA [CT]^GGCC[GA]
EcoNI 693 CCTTTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1768 GATATC GAT^ATC
HaeII 36 GGCGCT [GA]GCGC^[CT]
HincII 306 GTTGAC GT[CT]^[GA]AC
HindII 306 GTTGAC GT[CT]^[GA]AC
MamI 1768 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1707 AGATCC [GA]^GATC[CT]
MroI 1756 TCCGGA T^CCGGA
MslI 1009 CACTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 134 CCCGG CC^[GC]GG
NlaIV 840 GGTGCC GG[ACGT]^[ACGT]CC
NspV 281 TTCGAA TT^CGAA
PleI 438 GACTC GAGTC(4/5)
PstI 1421 CTGCAG CTGCA^G
RcaI 1149 TCATGA T^CATGA
SacI 1714 GAGCTC GAGCT^C
ScaI 1782 AGTACT AGT^ACT
SfcI 1421 CTGCAG C^T[GA][CT]AG
SfuI 281 TTCGAA TT^CGAA
XhoII 1707 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 10 CGCG CG^CG
27 CGCG
AclI 256 AACGTT AA^CGTT
1796 AACGTT
AlwI 526 GATCC GGATC(4/5)
1708 GATCC
BciVI 741 GTATCC GTATCC(6/5)
1467 GTATCC
BsaWI 168 ACCGGA [AT]^CCGG[AT]
1756 TCCGGA
BsmAI 973 GAGAC GTCTC(1/5)
1653 GTCTC
BsmFI 793 GTCCC GGGAC(10/14)
1682 GTCCC
BstUI 10 CGCG CG^CG
27 CGCG
DsaI 633 CCATGG C^C[GA][CT]GG
1520 CCATGG
MaeIII 1445 GTGAC ^GT[ACGT]AC
1729 GTCAC
MvnI 10 CGCG CG^CG
27 CGCG
NcoI 633 CCATGG C^CATGG
1520 CCATGG
PflMI 633 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
863 CCACAATCTGG
Psp1406I 256 AACGTT AA^CGTT
1796 AACGTT
SmlI 788 CTCAAG C^T[CT][GA]AG
1011 CTTGAG
Tsp45I 1445 GTGAC ^GT[GC]AC
1729 GTCAC
Van91I 633 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
863 CCACAATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BanII BbvI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I