Restriction map for
pF1KB5086
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AcsI AcyI AflII
AgeI AhdI AocI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsiWI BsmI
BspDI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I HindIII HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacI SbfI ScaI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1749 GTCGAC GT^[AC][GT]AC
AclI 1072 AACGTT AA^CGTT
AflIII 905 ACATGT A^C[GA][CT]GT
Alw44I 1510 GTGCAC G^TGCAC
Aor51HI 1379 AGCGCT AGC^GCT
ApaLI 1510 GTGCAC G^TGCAC
AviII 1568 TGCGCA TGC^GCA
BalI 1344 TGGCCA TGG^CCA
BanI 1675 GGCACC G^G[CT][GA]CC
BbsI 763 GTCTTC GAAGAC(2/6)
BbvCI 1885 CCTCAGC CCTCAGC(-5/-2)
BclI 1232 TGATCA T^GATCA
BpuAI 763 GTCTTC GAAGAC(2/6)
BsaI 247 GAGACC GGTCTC(1/5)
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 1778 GAGACG CGTCTC(1/5)
Bsp1407I 1590 TGTACA T^GTACA
BspHI 738 TCATGA T^CATGA
BspLU11I 905 ACATGT A^CATGT
BspMI 1622 ACCTGC ACCTGC(4/8)
BsrGI 1590 TGTACA T^GTACA
BstAPI 1354 GCATCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1933 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4 GACTCGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco47III 1379 AGCGCT AGC^GCT
Eco52I 71 CGGCCG C^GGCCG
FbaI 1232 TGATCA T^GATCA
FspI 1568 TGCGCA TGC^GCA
HgaI 1867 GCGTC GACGC(5/10)
HincII 1749 GTCGAC GT[CT]^[GA]AC
HindII 1749 GTCGAC GT[CT]^[GA]AC
KspI 65 CCGCGG CCGC^GG
MluNI 1344 TGGCCA TGG^CCA
MscI 1344 TGGCCA TGG^CCA
MseI 1739 TTAA T^TAA
NaeI 1910 GCCGGC GCC^GGC
NcoI 1505 CCATGG C^CATGG
NgoMIV 1910 GCCGGC G^CCGGC
NotI 70 GCGGCCGC GC^GGCCGC
PpuMI 506 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 904 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1072 AACGTT AA^CGTT
PstI 108 CTGCAG CTGCA^G
PvuII 1862 CAGCTG CAG^CTG
RcaI 738 TCATGA T^CATGA
SacII 65 CCGCGG CCGC^GG
SalI 1749 GTCGAC G^TCGAC
SapI 333 GAAGAGC GCTCTTC(1/4)
SexAI 413 ACCTGGT A^CC[AT]GGT
SfiI 63 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1590 TGTACA T^GTACA
Tru9I 1739 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 419 TCCGGA T^CCGGA
1391 TCCGGA
AlwI 981 GATCC GGATC(4/5)
271 GGATC
Asp700I 555 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1101 GAAGATGTTC
AspHI 1510 GTGCAC G[AT]GC[AT]^C
1814 GAGCAC
BfaI 172 CTAG C^TAG
1090 CTAG
BglI 64 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
662 GCCACGGCGGC
BsaWI 419 TCCGGA [AT]^CCGG[AT]
1391 TCCGGA
BseAI 419 TCCGGA T^CCGGA
1391 TCCGGA
BsgI 1624 CTGCAC GTGCAG(16/14)
994 GTGCAG
BsiHKAI 1510 GTGCAC G[AT]GC[AT]^C
1814 GAGCAC
BspEI 419 TCCGGA T^CCGGA
1391 TCCGGA
BsrFI 9 GCCGGT [GA]^CCGG[CT]
1910 GCCGGC
BsrI 861 ACTGG ACTGG(1/-1)
1573 ACTGG
BstXI 158 CCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
596 CCAGCAGTGTGG
Cfr10I 9 GCCGGT [GA]^CCGG[CT]
1910 GCCGGC
EarI 559 CTCTTC CTCTTC(1/4)
333 GAAGAG
Ksp632I 559 CTCTTC CTCTTC(1/4)
333 GAAGAG
MaeI 172 CTAG C^TAG
1090 CTAG
MroI 419 TCCGGA T^CCGGA
1391 TCCGGA
PflMI 1500 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1635 CCAGCGGCTGG
SmaI 48 CCCGGG CCC^GGG
1188 CCCGGG
SphI 797 GCATGC GCATG^C
1319 GCATGC
StuI 314 AGGCCT AGG^CCT
1227 AGGCCT
TaqI 725 TCGA T^CGA
1750 TCGA
Tsp509I 579 AATT ^AATT
1485 AATT
TthHB8I 725 TCGA T^CGA
1750 TCGA
Van91I 1500 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1635 CCAGCGGCTGG
XcmI 839 CCAAAGAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1541 CCATTCTGAGCATGG
XmaCI 48 CCCGGG C^CCGGG
1188 CCCGGG
XmaI 48 CCCGGG C^CCGGG
1188 CCCGGG
XmnI 555 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1101 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaI AvaII BanII BbvI
BcnI BglII BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI Tsp45I TspRI
XhoII