Restriction map for
pF1KB5078
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbrPI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaWI BseAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SfiI SfuI SmaI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 331 GTCGAC GT^[AC][GT]AC
AcsI 1180 GAATTT [GA]^AATT[CT]
Alw44I 909 GTGCAC G^TGCAC
AocI 547 CCTGAGG CC^T[ACGT]AGG
ApaLI 909 GTGCAC G^TGCAC
ApoI 1180 GAATTT [GA]^AATT[CT]
Asp700I 1311 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1668 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 15 CTCGGG C^[CT]CG[GA]G
AviII 2552 TGCGCA TGC^GCA
BbeI 1236 GGCGCC GGCGC^C
BbvCI 1582 GCTGAGG CCTCAGC(-5/-2)
BglII 248 AGATCT A^GATCT
BsaAI 2362 TACGTA [CT]AC^GT[GA]
BsaI 381 GGTCTC GGTCTC(1/5)
BsiEI 1257 CGGCCG CG[GA][CT]^CG
BsiWI 2364 CGTACG C^GTACG
BsoBI 15 CTCGGG C^[CT]CG[GA]G
BstAPI 1626 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1176 CCAGGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 547 CCTGAGG CC^T[ACGT]AGG
DraIII 2440 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1894 CCACGG C^C[GA][CT]GG
EagI 1257 CGGCCG C^GGCCG
EclXI 1257 CGGCCG C^GGCCG
Eco52I 1257 CGGCCG C^GGCCG
Eco81I 547 CCTGAGG CC^T[ACGT]AGG
FspI 2552 TGCGCA TGC^GCA
HgaI 1569 GACGC GACGC(5/10)
KasI 1236 GGCGCC G^GCGCC
NarI 1236 GGCGCC GG^CGCC
NspI 1301 ACATGC [GA]CATG^[CT]
PflFI 1668 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 331 GTCGAC G^TCGAC
SexAI 2441 ACCTGGT A^CC[AT]GGT
SfoI 1236 GGCGCC GGC^GCC
SgrAI 1571 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 2362 TACGTA TAC^GTA
TfiI 371 GAATC G^A[AT]TC
Tth111I 1668 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 488 CCATGAGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1311 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1236 GGCGCC G[GA]^CG[CT]C
1569 GACGCC
AflIII 152 ACGTGT A^C[GA][CT]GT
1593 ACGTGT
Aor51HI 1043 AGCGCT AGC^GCT
1739 AGCGCT
BanII 1936 GAGCTC G[GA]GC[CT]^C
2326 GAGCTC
BbsI 1566 GAAGAC GAAGAC(2/6)
1912 GAAGAC
BciVI 2544 GGATAC GTATCC(6/5)
957 GTATCC
BglI 74 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2249 GCCACGCAGGC
BpuAI 1566 GAAGAC GAAGAC(2/6)
1912 GAAGAC
BsaBI 1329 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1440 GATGCCTATC
BsaHI 1236 GGCGCC G[GA]^CG[CT]C
1569 GACGCC
BseRI 1939 CTCCTC GAGGAG(10/8)
1123 GAGGAG
BsmI 416 GAATGC GAATGC(1/-1)
1146 GAATGC
BsrBI 528 CCGCTC CCGCTC(-3/-3)
1106 CCGCTC
BsrDI 1272 CATTGC GCAATG(2/0)
2495 GCAATG
Eco47III 1043 AGCGCT AGC^GCT
1739 AGCGCT
EcoNI 48 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
352 CCTGGGGAAGG
Hin1I 1236 GGCGCC G[GA]^CG[CT]C
1569 GACGCC
MamI 1329 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1440 GATGCCTATC
NaeI 74 GCCGGC GCC^GGC
1233 GCCGGC
NgoMIV 74 GCCGGC G^CCGGC
1233 GCCGGC
NheI 396 GCTAGC G^CTAGC
859 GCTAGC
PpuMI 9 GGGACCC [GA]G^G[AT]CC[CT]
2504 GGGACCC
PstI 49 CTGCAG CTGCA^G
1537 CTGCAG
SacI 1936 GAGCTC GAGCT^C
2326 GAGCTC
SfcI 49 CTGCAG C^T[GA][CT]AG
1537 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII