Restriction map for
pF1KB5072
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HpaI KasI KpnI
Ksp632I KspI MamI MluI MroI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 949 GTAGAC GT^[AC][GT]AC
AccII 119 CGCG CG^CG
AcyI 982 GACGCC G[GA]^CG[CT]C
AlwNI 216 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 599 GTGCTC G[AT]GC[AT]^C
BalI 1394 TGGCCA TGG^CCA
BanII 1139 GAGCCC G[GA]GC[CT]^C
BciVI 51 GGATAC GTATCC(6/5)
BclI 1473 TGATCA T^GATCA
BcnI 1055 CCGGG CC^[GC]GG
BglI 25 GCCATTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 12 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC
BsaHI 982 GACGCC G[GA]^CG[CT]C
BsaI 1735 GAGACC GGTCTC(1/5)
BseRI 383 CTCCTC GAGGAG(10/8)
BsiHKAI 599 GTGCTC G[AT]GC[AT]^C
BsmI 1689 GAATGC GAATGC(1/-1)
BspMI 505 GCAGGT ACCTGC(4/8)
BsrBI 1293 GAGCGG CCGCTC(-3/-3)
BstUI 119 CGCG CG^CG
BstXI 1766 CCACTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 12 GCTGAGC GC^T[ACGT]AGC
CfoI 391 GCGC GCG^C
DsaI 1787 CCGTGG C^C[GA][CT]GG
FbaI 1473 TGATCA T^GATCA
HaeII 390 AGCGCC [GA]GCGC^[CT]
HhaI 391 GCGC GCG^C
Hin1I 982 GACGCC G[GA]^CG[CT]C
HinP1I 391 GCGC G^CGC
HincII 679 GTTGAC GT[CT]^[GA]AC
HindII 679 GTTGAC GT[CT]^[GA]AC
MaeII 1778 ACGT A^CGT
MfeI 208 CAATTG C^AATTG
MluNI 1394 TGGCCA TGG^CCA
MscI 1394 TGGCCA TGG^CCA
MunI 208 CAATTG C^AATTG
MvnI 119 CGCG CG^CG
NciI 1055 CCGGG CC^[GC]GG
NspI 1427 GCATGT [GA]CATG^[CT]
PaeR7I 922 CTCGAG C^TCGAG
PpuMI 507 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 849 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 503 CTGCAG CTGCA^G
SfiI 24 GGCCATTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 922 CTCGAG C^T[CT][GA]AG
SspI 1555 AATATT AAT^ATT
StuI 1220 AGGCCT AGG^CCT
XbaI 1111 TCTAGA T^CTAGA
XcmI 426 CCAGCCGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 922 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1099 AAATTT [GA]^AATT[CT]
1516 GAATTT
ApoI 1099 AAATTT [GA]^AATT[CT]
1516 GAATTT
Asp700I 449 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
657 GAAGCTTTTC
AvaII 508 GGTCC G^G[AT]CC
590 GGACC
BanI 552 GGTGCC G^G[CT][GA]CC
778 GGCACC
BbsI 979 GAAGAC GAAGAC(2/6)
1182 GTCTTC
BfaI 670 CTAG C^TAG
1112 CTAG
BpmI 386 CTCCAG CTGGAG(16/14)
633 CTGGAG
BpuAI 979 GAAGAC GAAGAC(2/6)
1182 GTCTTC
BsmFI 92 GTCCC GGGAC(10/14)
509 GTCCC
BspHI 134 TCATGA T^CATGA
1820 TCATGA
BssSI 193 CTCGTG CACGAG(-5/-1)
602 CTCGTG
BstYI 623 AGATCC [GA]^GATC[CT]
962 GGATCT
DraII 421 AGGCCCC [GA]G^G[ACGT]CC[CT]
507 AGGTCCC
EcoO109I 421 AGGCCCC [GA]G^G[ACGT]CC[CT]
507 AGGTCCC
EcoT14I 1362 CCAAGG C^C[AT][AT]GG
1617 CCTTGG
HapII 1055 CCGG C^CGG
1375 CCGG
HindIII 658 AAGCTT A^AGCTT
1228 AAGCTT
HpaII 1055 CCGG C^CGG
1375 CCGG
MaeI 670 CTAG C^TAG
1112 CTAG
MflI 623 AGATCC [GA]^GATC[CT]
962 GGATCT
MslI 1398 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1767 CACTGTGATG
MspI 1055 CCGG C^CGG
1375 CCGG
RcaI 134 TCATGA T^CATGA
1820 TCATGA
StyI 1362 CCAAGG C^C[AT][AT]GG
1617 CCTTGG
XhoII 623 AGATCC [GA]^GATC[CT]
962 GGATCT
XmnI 449 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
657 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BbvI BmyI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HgaI HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I