Restriction map for
pF1KB5066
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI
BstXI Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 600 GTATAC GT^[AC][GT]AC
AccII 1257 CGCG CG^CG
AcsI 478 GAATTT [GA]^AATT[CT]
AcyI 1305 GACGCC G[GA]^CG[CT]C
AhdI 168 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 478 GAATTT [GA]^AATT[CT]
AspEI 168 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 140 GGATCC G^GATCC
BanI 1451 GGCACC G^G[CT][GA]CC
BfaI 584 CTAG C^TAG
BglI 1482 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 578 AGATCT A^GATCT
BsaHI 1305 GACGCC G[GA]^CG[CT]C
BsaI 166 GAGACC GGTCTC(1/5)
BsmFI 238 GGGAC GGGAC(10/14)
Bst1107I 600 GTATAC GTA^TAC
BstEII 175 GGTCACC G^GT[ACGT]ACC
BstPI 175 GGTCACC G^GT[ACGT]ACC
BstUI 1257 CGCG CG^CG
BstZ17I 600 GTATAC GTA^TAC
CfoI 912 GCGC GCG^C
DsaI 1485 CCATGG C^C[GA][CT]GG
Eam1105I 168 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1514 CCTCGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 175 GGTCACC G^GT[ACGT]ACC
EcoT14I 1485 CCATGG C^C[AT][AT]GG
HhaI 912 GCGC GCG^C
Hin1I 1305 GACGCC G[GA]^CG[CT]C
HinP1I 912 GCGC G^CGC
MaeI 584 CTAG C^TAG
MseI 797 TTAA T^TAA
MvnI 1257 CGCG CG^CG
NcoI 1485 CCATGG C^CATGG
PaeR7I 1515 CTCGAG C^TCGAG
PflMI 435 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 192 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 1675 GAAGAGC GCTCTTC(1/4)
SexAI 1532 ACCTGGT A^CC[AT]GGT
SgrAI 47 CGCCGGCG C[GA]^CCGG[CT]G
StyI 1485 CCATGG C^C[AT][AT]GG
Tru9I 797 TTAA T^TAA
Van91I 435 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 583 TCTAGA T^CTAGA
XhoI 1515 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 776 GTAC GT^AC
974 GTAC
Alw44I 820 GTGCAC G^TGCAC
1657 GTGCAC
AlwI 141 GATCC GGATC(4/5)
140 GGATC
ApaLI 820 GTGCAC G^TGCAC
1657 GTGCAC
AvaI 7 CCCGAG C^[CT]CG[GA]G
1515 CTCGAG
BsgI 1156 CTGCAC GTGCAG(16/14)
754 GTGCAG
BsmAI 166 GAGAC GTCTC(1/5)
926 GAGAC
BsoBI 7 CCCGAG C^[CT]CG[GA]G
1515 CTCGAG
BspMI 179 ACCTGC ACCTGC(4/8)
1108 ACCTGC
BsrFI 48 GCCGGC [GA]^CCGG[CT]
1496 GCCGGC
BstYI 140 GGATCC [GA]^GATC[CT]
578 AGATCT
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
1496 GCCGGC
HgaI 1305 GACGC GACGC(5/10)
545 GCGTC
MflI 140 GGATCC [GA]^GATC[CT]
578 AGATCT
NaeI 48 GCCGGC GCC^GGC
1496 GCCGGC
NgoMIV 48 GCCGGC G^CCGGC
1496 GCCGGC
PstI 1110 CTGCAG CTGCA^G
1571 CTGCAG
PvuII 121 CAGCTG CAG^CTG
226 CAGCTG
RsaI 776 GTAC GT^AC
974 GTAC
SacI 1434 GAGCTC GAGCT^C
1566 GAGCTC
SfcI 1110 CTGCAG C^T[GA][CT]AG
1571 CTGCAG
TaqI 300 TCGA T^CGA
1516 TCGA
Tsp509I 479 AATT ^AATT
729 AATT
TthHB8I 300 TCGA T^CGA
1516 TCGA
XhoII 140 GGATCC [GA]^GATC[CT]
578 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwNI Asp700I AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI TseI
Tsp45I TspRI XmnI