Restriction map for
pF1KB5053
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvrII BbrPI
BbvCI BciVI BclI BfrI BlnI
BsaAI BseRI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI DraI
DrdI Eam1105I EarI Eco47III Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacI SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 814 GTAGAC GT^[AC][GT]AC
AccIII 1578 TCCGGA T^CCGGA
AcsI 961 GAATTC [GA]^AATT[CT]
AcyI 3 GGCGCC G[GA]^CG[CT]C
ApaI 1051 GGGCCC GGGCC^C
ApoI 961 GAATTC [GA]^AATT[CT]
Asp700I 2187 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1895 TGCGCA TGC^GCA
BbeI 3 GGCGCC GGCGC^C
BbsI 937 GAAGAC GAAGAC(2/6)
BcgI 1452 CGAGACCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1301 AGATCT A^GATCT
BlpI 1184 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1184 GCTCAGC GC^T[ACGT]AGC
BpuAI 937 GAAGAC GAAGAC(2/6)
BsaBI 1749 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BseAI 1578 TCCGGA T^CCGGA
BspEI 1578 TCCGGA T^CCGGA
BsrBI 1282 GAGCGG CCGCTC(-3/-3)
BssSI 2083 CACGAG CACGAG(-5/-1)
BstXI 801 CCACGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1184 GCTCAGC GC^T[ACGT]AGC
CpoI 278 CGGACCG CG^G[AT]CCG
EcoRI 961 GAATTC G^AATTC
FspI 1895 TGCGCA TGC^GCA
Hin1I 3 GGCGCC G[GA]^CG[CT]C
KasI 3 GGCGCC G^GCGCC
MamI 1749 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1578 TCCGGA T^CCGGA
NarI 3 GGCGCC GG^CGCC
NcoI 1215 CCATGG C^CATGG
PaeR7I 2004 CTCGAG C^TCGAG
RsrII 278 CGGACCG CG^G[AT]CCG
SfiI 306 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3 GGCGCC GGC^GCC
SphI 2016 GCATGC GCATG^C
TaqI 2005 TCGA T^CGA
TthHB8I 2005 TCGA T^CGA
XhoI 2004 CTCGAG C^TCGAG
XmnI 2187 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 1270 CGCG CG^CG
2299 CGCG
AvaI 1450 CCCGAG C^[CT]CG[GA]G
2004 CTCGAG
BamHI 656 GGATCC G^GATCC
2078 GGATCC
BfaI 142 CTAG C^TAG
952 CTAG
BsaWI 715 TCCGGT [AT]^CCGG[AT]
1578 TCCGGA
BsmBI 1982 GAGACG CGTCTC(1/5)
2278 GAGACG
BsoBI 1450 CCCGAG C^[CT]CG[GA]G
2004 CTCGAG
BsrFI 179 ACCGGC [GA]^CCGG[CT]
928 ACCGGC
BstUI 1270 CGCG CG^CG
2299 CGCG
Cfr10I 179 ACCGGC [GA]^CCGG[CT]
928 ACCGGC
EcoNI 1412 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1752 CCTCATCAAGG
HgaI 1441 GACGC GACGC(5/10)
2280 GACGC
MaeI 142 CTAG C^TAG
952 CTAG
MaeII 1471 ACGT A^CGT
1670 ACGT
MseI 155 TTAA T^TAA
1019 TTAA
MvnI 1270 CGCG CG^CG
2299 CGCG
NspI 1662 GCATGT [GA]CATG^[CT]
2016 GCATGC
PvuII 1190 CAGCTG CAG^CTG
1793 CAGCTG
TfiI 512 GAATC G^A[AT]TC
2151 GATTC
Tru9I 155 TTAA T^TAA
1019 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaI
BsaJI BsgI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TseI
Tsp45I Tsp509I TspRI XcmI XhoII