Restriction map for
pF1KB5048
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 664 GACGTC GACGT^C
AccI 715 GTCTAC GT^[AC][GT]AC
AcyI 664 GACGTC G[GA]^CG[CT]C
AflIII 701 ACACGT A^C[GA][CT]GT
Alw44I 1591 GTGCAC G^TGCAC
AocI 330 CCTCAGG CC^T[ACGT]AGG
ApaLI 1591 GTGCAC G^TGCAC
Asp700I 913 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1257 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1678 GGATCC G^GATCC
BanI 835 GGCACC G^G[CT][GA]CC
BglII 2096 AGATCT A^GATCT
BsaAI 702 CACGTA [CT]AC^GT[GA]
BsaHI 664 GACGTC G[GA]^CG[CT]C
BsaI 1441 GAGACC GGTCTC(1/5)
BsgI 1927 CTGCAC GTGCAG(16/14)
BsmI 1140 GAATGC GAATGC(1/-1)
BspDI 1387 ATCGAT AT^CGAT
BspHI 1232 TCATGA T^CATGA
BsrBI 1956 CCGCTC CCGCTC(-3/-3)
BsrFI 1801 GCCGGC [GA]^CCGG[CT]
BstEII 381 GGTGACC G^GT[ACGT]ACC
BstPI 381 GGTGACC G^GT[ACGT]ACC
Bsu36I 330 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1801 GCCGGC [GA]^CCGG[CT]
ClaI 1387 ATCGAT AT^CGAT
DsaI 959 CCACGG C^C[GA][CT]GG
EarI 1183 GAAGAG CTCTTC(1/4)
Eco81I 330 CCTCAGG CC^T[ACGT]AGG
EcoO65I 381 GGTGACC G^GT[ACGT]ACC
EcoT22I 670 ATGCAT ATGCA^T
FseI 1800 GGCCGGCC GGCCGG^CC
HaeII 875 GGCGCT [GA]GCGC^[CT]
Hin1I 664 GACGTC G[GA]^CG[CT]C
Ksp632I 1183 GAAGAG CTCTTC(1/4)
MspA1I 392 CAGCGG C[AC]G^C[GT]G
NaeI 1801 GCCGGC GCC^GGC
NgoMIV 1801 GCCGGC G^CCGGC
NsiI 670 ATGCAT ATGCA^T
NspI 1380 GCATGT [GA]CATG^[CT]
PflFI 1257 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1830 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1232 TCATGA T^CATGA
SmaI 1754 CCCGGG CCC^GGG
TfiI 1196 GATTC G^A[AT]TC
Tth111I 1257 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1754 CCCGGG C^CCGGG
XmaI 1754 CCCGGG C^CCGGG
XmnI 913 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 376 CGCG CG^CG
1636 CGCG
AfaI 1188 GTAC GT^AC
1809 GTAC
AspHI 67 GAGCAC G[AT]GC[AT]^C
1591 GTGCAC
BalI 728 TGGCCA TGG^CCA
890 TGGCCA
BsiHKAI 67 GAGCAC G[AT]GC[AT]^C
1591 GTGCAC
BstUI 376 CGCG CG^CG
1636 CGCG
EcoNI 408 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1341 CCTGAGCCAGG
MluNI 728 TGGCCA TGG^CCA
890 TGGCCA
MscI 728 TGGCCA TGG^CCA
890 TGGCCA
MseI 1504 TTAA T^TAA
1982 TTAA
MvnI 376 CGCG CG^CG
1636 CGCG
PflMI 795 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1511 CCAAGGACTGG
PleI 1552 GACTC GAGTC(4/5)
2168 GACTC
PpuMI 896 AGGACCT [GA]G^G[AT]CC[CT]
2126 AGGACCT
PstI 1037 CTGCAG CTGCA^G
1912 CTGCAG
RsaI 1188 GTAC GT^AC
1809 GTAC
SfcI 1037 CTGCAG C^T[GA][CT]AG
1912 CTGCAG
Tru9I 1504 TTAA T^TAA
1982 TTAA
Tsp509I 100 AATT ^AATT
939 AATT
Van91I 795 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1511 CCAAGGACTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaI AvaII BanII BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII