Restriction map for
pF1KB5010
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 1038 GGCGTC G[GA]^CG[CT]C
AlwI 468 GATCC GGATC(4/5)
AlwNI 1276 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1218 CCTGAGG CC^T[ACGT]AGG
AvrII 241 CCTAGG C^CTAGG
BanI 285 GGCACC G^G[CT][GA]CC
BbrPI 717 CACGTG CAC^GTG
BlnI 241 CCTAGG C^CTAGG
BsaAI 717 CACGTG [CT]AC^GT[GA]
BsaHI 1038 GGCGTC G[GA]^CG[CT]C
BsaI 1567 GAGACC GGTCTC(1/5)
BsrDI 1017 GCAATG GCAATG(2/0)
BstAPI 1637 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 467 AGATCC [GA]^GATC[CT]
Bsu36I 1218 CCTGAGG CC^T[ACGT]AGG
DpnII 468 GATC ^GATC
Eco81I 1218 CCTGAGG CC^T[ACGT]AGG
EcoNI 1363 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1850 GGCCGGCC GGCCGG^CC
Hin1I 1038 GGCGTC G[GA]^CG[CT]C
HinfI 1393 GAGTC G^A[ACGT]TC
MboI 468 GATC ^GATC
MflI 467 AGATCC [GA]^GATC[CT]
NcoI 1790 CCATGG C^CATGG
NdeII 468 GATC ^GATC
PaeR7I 876 CTCGAG C^TCGAG
PflMI 1412 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1393 GAGTC GAGTC(4/5)
PmaCI 717 CACGTG CAC^GTG
PmlI 717 CACGTG CAC^GTG
PstI 14 CTGCAG CTGCA^G
PvuII 588 CAGCTG CAG^CTG
SapI 690 GAAGAGC GCTCTTC(1/4)
Sau3AI 468 GATC ^GATC
Tsp45I 1022 GTGAC ^GT[GC]AC
Van91I 1412 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 876 CTCGAG C^TCGAG
XhoII 467 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 80 GTAC GT^AC
563 GTAC
Aor51HI 47 AGCGCT AGC^GCT
1604 AGCGCT
Asp700I 690 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
831 GAAGCCCTTC
BglI 482 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1330 GCCGCCCCGGC
BpmI 1364 CTCCAG CTGGAG(16/14)
1810 CTGGAG
BseRI 1099 GAGGAG GAGGAG(10/8)
1519 GAGGAG
BsgI 1271 CTGCAC GTGCAG(16/14)
1635 CTGCAC
BsmAI 181 GAGAC GTCTC(1/5)
1567 GAGAC
BsmI 1453 GAATGC GAATGC(1/-1)
1835 GAATGC
BstF5I 707 CATCC GGATG(2/0)
732 CATCC
Eco47III 47 AGCGCT AGC^GCT
1604 AGCGCT
FokI 707 CATCC GGATG(9/13)
732 CATCC
KspI 230 CCGCGG CCGC^GG
1256 CCGCGG
MaeII 718 ACGT A^CGT
1030 ACGT
MaeIII 1022 GTGAC ^GT[ACGT]AC
1181 GTAAC
NotI 350 GCGGCCGC GC^GGCCGC
854 GCGGCCGC
RsaI 80 GTAC GT^AC
563 GTAC
SacI 619 GAGCTC GAGCT^C
1618 GAGCTC
SacII 230 CCGCGG CCGC^GG
1256 CCGCGG
SfaNI 1462 GATGC GCATC(5/9)
731 GCATC
SfcI 14 CTGCAG C^T[GA][CT]AG
585 CTACAG
XmnI 690 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
831 GAAGCCCTTC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AluI Alw44I ApaLI
AspHI AvaI AvaII AviII BalI
BanII BbvI BcnI BfaI BmyI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV NspI PpuMI
Sau96I ScrFI SgrAI SmlI StuI
StyI TaqI TseI TspRI TthHB8I