Restriction map for
pF1KB4596
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BcgI BfrI BglI BlnI
BsgI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraIII Eam1105I Eco47III Eco81I
EcoO65I EcoT22I FseI FspI HpaI
KpnI KspI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SbfI ScaI SfcI SfiI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1234 GTCGAC GT^[AC][GT]AC
AcyI 1171 GGCGCC G[GA]^CG[CT]C
AflIII 2660 ACGTGT A^C[GA][CT]GT
Alw44I 355 GTGCAC G^TGCAC
AlwNI 13 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 355 GTGCAC G^TGCAC
Asp700I 2289 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 355 GTGCAC G[AT]GC[AT]^C
BalI 938 TGGCCA TGG^CCA
BamHI 850 GGATCC G^GATCC
BanII 2005 GAGCCC G[GA]GC[CT]^C
BbeI 1171 GGCGCC GGCGC^C
BbrPI 1851 CACGTG CAC^GTG
BbvCI 1494 GCTGAGG CCTCAGC(-5/-2)
BciVI 2447 GTATCC GTATCC(6/5)
BlpI 1142 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1142 GCTCAGC GC^T[ACGT]AGC
BsaAI 1851 CACGTG [CT]AC^GT[GA]
BsaHI 1171 GGCGCC G[GA]^CG[CT]C
BsiEI 2714 CGGCCG CG[GA][CT]^CG
BsiHKAI 355 GTGCAC G[AT]GC[AT]^C
BspMI 2434 ACCTGC ACCTGC(4/8)
BsrBI 531 GAGCGG CCGCTC(-3/-3)
BstAPI 2045 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 269 TTCGAA TT^CGAA
BstXI 1148 CCAACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1142 GCTCAGC GC^T[ACGT]AGC
DraI 523 TTTAAA TTT^AAA
DrdI 2549 GACTGGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2714 CGGCCG C^GGCCG
EclXI 2714 CGGCCG C^GGCCG
Eco52I 2714 CGGCCG C^GGCCG
EcoRI 272 GAATTC G^AATTC
Hin1I 1171 GGCGCC G[GA]^CG[CT]C
HindIII 2596 AAGCTT A^AGCTT
KasI 1171 GGCGCC G^GCGCC
MluNI 938 TGGCCA TGG^CCA
MscI 938 TGGCCA TGG^CCA
NarI 1171 GGCGCC GG^CGCC
NcoI 1361 CCATGG C^CATGG
NspV 269 TTCGAA TT^CGAA
PmaCI 1851 CACGTG CAC^GTG
PmlI 1851 CACGTG CAC^GTG
SalI 1234 GTCGAC G^TCGAC
SapI 1714 GAAGAGC GCTCTTC(1/4)
SexAI 50 ACCAGGT A^CC[AT]GGT
SfoI 1171 GGCGCC GGC^GCC
SfuI 269 TTCGAA TT^CGAA
SspI 2192 AATATT AAT^ATT
StuI 1041 AGGCCT AGG^CCT
XcmI 1781 CCAGGATGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2289 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 818 TCCGGA T^CCGGA
1547 TCCGGA
AcsI 272 GAATTC [GA]^AATT[CT]
1936 GAATTT
AfaI 900 GTAC GT^AC
2244 GTAC
ApoI 272 GAATTC [GA]^AATT[CT]
1936 GAATTT
AspI 49 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1099 GACTGTGTC
BfaI 928 CTAG C^TAG
2419 CTAG
BglII 1673 AGATCT A^GATCT
2312 AGATCT
BsaI 1414 GAGACC GGTCTC(1/5)
2347 GAGACC
BsaWI 818 TCCGGA [AT]^CCGG[AT]
1547 TCCGGA
BseAI 818 TCCGGA T^CCGGA
1547 TCCGGA
BspEI 818 TCCGGA T^CCGGA
1547 TCCGGA
BspHI 116 TCATGA T^CATGA
294 TCATGA
BsrDI 240 CATTGC GCAATG(2/0)
2045 GCAATG
BsrFI 1400 ACCGGC [GA]^CCGG[CT]
2038 ACCGGC
Cfr10I 1400 ACCGGC [GA]^CCGG[CT]
2038 ACCGGC
EcoNI 1904 CCTTACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2673 CCTGTCTGAGG
EcoRV 112 GATATC GAT^ATC
2158 GATATC
EcoT14I 941 CCAAGG C^C[AT][AT]GG
1361 CCATGG
HaeII 1171 GGCGCC [GA]GCGC^[CT]
1295 GGCGCT
HincII 1234 GTCGAC GT[CT]^[GA]AC
1963 GTCAAC
HindII 1234 GTCGAC GT[CT]^[GA]AC
1963 GTCAAC
MaeI 928 CTAG C^TAG
2419 CTAG
MroI 818 TCCGGA T^CCGGA
1547 TCCGGA
NspI 896 GCATGT [GA]CATG^[CT]
1445 ACATGC
PflFI 49 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1099 GACTGTGTC
PvuII 871 CAGCTG CAG^CTG
2494 CAGCTG
RcaI 116 TCATGA T^CATGA
294 TCATGA
RsaI 900 GTAC GT^AC
2244 GTAC
SmaI 155 CCCGGG CCC^GGG
2333 CCCGGG
StyI 941 CCAAGG C^C[AT][AT]GG
1361 CCATGG
Tth111I 49 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1099 GACTGTGTC
XmaCI 155 CCCGGG C^CCGGG
2333 CCCGGG
XmaI 155 CCCGGG C^CCGGG
2333 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BanI BbsI BbvI BclI
BcnI BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII