Restriction map for
pF1KB4430
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BamHI BanI BbeI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HaeII Hin1I
HindIII KasI KpnI Ksp632I KspI
MfeI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1339 GTCTAC GT^[AC][GT]AC
AfaI 1005 GTAC GT^AC
Alw44I 1126 GTGCAC G^TGCAC
AlwI 69 GATCC GGATC(4/5)
ApaLI 1126 GTGCAC G^TGCAC
AviII 1040 TGCGCA TGC^GCA
BanII 1143 GAGCTC G[GA]GC[CT]^C
BbrPI 687 CACGTG CAC^GTG
BglII 830 AGATCT A^GATCT
BsaAI 687 CACGTG [CT]AC^GT[GA]
BsaBI 1116 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 428 GCAATG GCAATG(2/0)
BssSI 1156 CTCGTG CACGAG(-5/-1)
BstYI 830 AGATCT [GA]^GATC[CT]
CfoI 1041 GCGC GCG^C
DrdI 103 GACATGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1346 CCATGG C^C[GA][CT]GG
EaeI 28 TGGCCG [CT]^GGCC[GA]
FauI 1434 GCGGG CCCGC(4/6)
FspI 1040 TGCGCA TGC^GCA
HhaI 1041 GCGC GCG^C
HinP1I 1041 GCGC G^CGC
HpaI 571 GTTAAC GTT^AAC
MamI 1116 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 830 AGATCT [GA]^GATC[CT]
MluI 52 ACGCGT A^CGCGT
NcoI 1346 CCATGG C^CATGG
NspI 1017 GCATGT [GA]CATG^[CT]
PleI 1318 GAGTC GAGTC(4/5)
PmaCI 687 CACGTG CAC^GTG
PmlI 687 CACGTG CAC^GTG
PshAI 1332 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 210 CTGCAG CTGCA^G
PvuII 1026 CAGCTG CAG^CTG
RsaI 1005 GTAC GT^AC
SacI 1143 GAGCTC GAGCT^C
SmaI 1064 CCCGGG CCC^GGG
SmlI 1138 CTCAAG C^T[CT][GA]AG
XcmI 1295 CCAGGATGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 830 AGATCT [GA]^GATC[CT]
XmaCI 1064 CCCGGG C^CCGGG
XmaI 1064 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 1064 CCCGGG C^[CT]CG[GA]G
1641 CTCGGG
BbvCI 766 GCTGAGG CCTCAGC(-5/-2)
928 GCTGAGG
BcgI 639 CGAGCACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1052 GCAGCTCCCTCG
BclI 968 TGATCA T^GATCA
1089 TGATCA
BcnI 1064 CCCGG CC^[GC]GG
1065 CCGGG
BsgI 34 CTGCAC GTGCAG(16/14)
128 GTGCAG
BsmBI 1582 CGTCTC CGTCTC(1/5)
1543 GAGACG
BsmFI 1133 GGGAC GGGAC(10/14)
1562 GGGAC
BsoBI 1064 CCCGGG C^[CT]CG[GA]G
1641 CTCGGG
BsrBI 669 CCGCTC CCGCTC(-3/-3)
934 GAGCGG
BsrFI 575 ACCGGC [GA]^CCGG[CT]
607 ACCGGC
Cfr10I 575 ACCGGC [GA]^CCGG[CT]
607 ACCGGC
FbaI 968 TGATCA T^GATCA
1089 TGATCA
HincII 571 GTTAAC GT[CT]^[GA]AC
1548 GTTGAC
HindII 571 GTTAAC GT[CT]^[GA]AC
1548 GTTGAC
MseI 95 TTAA T^TAA
572 TTAA
MspA1I 31 CCGCTG C[AC]G^C[GT]G
1026 CAGCTG
NciI 1064 CCCGG CC^[GC]GG
1065 CCGGG
PflMI 1502 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1593 CCAGTGTGTGG
PpuMI 1034 AGGACCT [GA]G^G[AT]CC[CT]
1133 GGGACCT
TaqI 1451 TCGA T^CGA
1482 TCGA
TfiI 1291 GAATC G^A[AT]TC
1668 GATTC
Tru9I 95 TTAA T^TAA
572 TTAA
TthHB8I 1451 TCGA T^CGA
1482 TCGA
Van91I 1502 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1593 CCAGTGTGTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcsI AflIII AluI
AlwNI ApoI AspHI AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I Tsp509I TspRI