Restriction map for
pF1KB4366
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiWI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII HindIII HpaI KasI KspI
MluI MroI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1885 GGTACC G^GTACC
AccII 520 CGCG CG^CG
AcyI 149 GGCGTC G[GA]^CG[CT]C
AflIII 1651 ACATGT A^C[GA][CT]GT
Alw44I 1974 GTGCAC G^TGCAC
ApaLI 1974 GTGCAC G^TGCAC
AseI 435 ATTAAT AT^TAAT
AsnI 435 ATTAAT AT^TAAT
Asp718I 1885 GGTACC G^GTACC
BanI 1885 GGTACC G^G[CT][GA]CC
BanII 1288 GAGCCC G[GA]GC[CT]^C
BbsI 2629 GAAGAC GAAGAC(2/6)
BclI 1473 TGATCA T^GATCA
BcnI 668 CCGGG CC^[GC]GG
BglII 1821 AGATCT A^GATCT
BpuAI 2629 GAAGAC GAAGAC(2/6)
BsaHI 149 GGCGTC G[GA]^CG[CT]C
BsaI 2666 GAGACC GGTCTC(1/5)
BsmBI 2481 CGTCTC CGTCTC(1/5)
BsmI 1213 GCATTC GAATGC(1/-1)
BspDI 2102 ATCGAT AT^CGAT
BspLU11I 1651 ACATGT A^CATGT
BsrBI 1140 GAGCGG CCGCTC(-3/-3)
BsrDI 687 CATTGC GCAATG(2/0)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BssSI 86 CACGAG CACGAG(-5/-1)
BstUI 520 CGCG CG^CG
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
ClaI 2102 ATCGAT AT^CGAT
DraII 1305 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1305 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 2670 CCTTGG C^C[AT][AT]GG
FbaI 1473 TGATCA T^GATCA
HgaI 150 GCGTC GACGC(5/10)
Hin1I 149 GGCGTC G[GA]^CG[CT]C
HincII 556 GTTGAC GT[CT]^[GA]AC
HindII 556 GTTGAC GT[CT]^[GA]AC
KpnI 1885 GGTACC GGTAC^C
MaeII 851 ACGT A^CGT
MfeI 299 CAATTG C^AATTG
MunI 299 CAATTG C^AATTG
MvnI 520 CGCG CG^CG
NaeI 13 GCCGGC GCC^GGC
NciI 668 CCGGG CC^[GC]GG
NgoMIV 13 GCCGGC G^CCGGC
PaeR7I 221 CTCGAG C^TCGAG
PpuMI 1305 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 435 ATTAAT AT^TAAT
PvuI 2104 CGATCG CGAT^CG
SapI 2689 GAAGAGC GCTCTTC(1/4)
SspI 2058 AATATT AAT^ATT
StuI 835 AGGCCT AGG^CCT
StyI 2670 CCTTGG C^C[AT][AT]GG
XhoI 221 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 1655 GTGCTC G[AT]GC[AT]^C
1974 GTGCAC
AvaI 221 CTCGAG C^[CT]CG[GA]G
2043 CTCGGG
BalI 1769 TGGCCA TGG^CCA
2173 TGGCCA
BciVI 2457 GGATAC GTATCC(6/5)
2277 GTATCC
BfaI 1825 CTAG C^TAG
2149 CTAG
BsgI 1201 GTGCAG GTGCAG(16/14)
1739 GTGCAG
BsiHKAI 1655 GTGCTC G[AT]GC[AT]^C
1974 GTGCAC
BsoBI 221 CTCGAG C^[CT]CG[GA]G
2043 CTCGGG
BspMI 1668 GCAGGT ACCTGC(4/8)
1790 GCAGGT
BstXI 2585 CCACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2729 CCACCCTTCTGG
CfoI 519 GCGC GCG^C
1869 GCGC
EcoNI 1297 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2733 CCTTCTGGAGG
EcoRV 2197 GATATC GAT^ATC
2373 GATATC
HapII 14 CCGG C^CGG
668 CCGG
HhaI 519 GCGC GCG^C
1869 GCGC
HinP1I 519 GCGC G^CGC
1869 GCGC
HpaII 14 CCGG C^CGG
668 CCGG
MaeI 1825 CTAG C^TAG
2149 CTAG
MluNI 1769 TGGCCA TGG^CCA
2173 TGGCCA
MscI 1769 TGGCCA TGG^CCA
2173 TGGCCA
MspI 14 CCGG C^CGG
668 CCGG
NdeI 617 CATATG CA^TATG
991 CATATG
NspI 1105 ACATGC [GA]CATG^[CT]
1651 ACATGT
PstI 458 CTGCAG CTGCA^G
886 CTGCAG
PvuII 356 CAGCTG CAG^CTG
2552 CAGCTG
ScaI 46 AGTACT AGT^ACT
702 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BmyI BpmI
BsaBI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII