Restriction map for
pF1KB4351
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HgaI HindIII
HpaI HphI KpnI MfeI MluI
MroI MslI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TspRI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 430 AACGTT AA^CGTT
AcyI 100 GGCGCC G[GA]^CG[CT]C
AflIII 568 ACACGT A^C[GA][CT]GT
AlwNI 916 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 27 GGGCCC GGGCC^C
AvaI 954 CCCGAG C^[CT]CG[GA]G
BalI 563 TGGCCA TGG^CCA
BamHI 733 GGATCC G^GATCC
BbeI 100 GGCGCC GGCGC^C
BbvCI 360 CCTCAGC CCTCAGC(-5/-2)
BciVI 1069 GGATAC GTATCC(6/5)
BclI 534 TGATCA T^GATCA
BcnI 30 CCCGG CC^[GC]GG
BsaHI 100 GGCGCC G[GA]^CG[CT]C
BsgI 436 CTGCAC GTGCAG(16/14)
BsmAI 1048 GAGAC GTCTC(1/5)
BsmI 647 GCATTC GAATGC(1/-1)
BsoBI 954 CCCGAG C^[CT]CG[GA]G
BsrDI 365 GCAATG GCAATG(2/0)
BstAPI 110 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1005 GGATG GGATG(2/0)
BstXI 851 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 771 TTTAAA TTT^AAA
DraII 314 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 563 TGGCCA [CT]^GGCC[GA]
EcoO109I 314 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 600 GAATTC G^AATTC
FbaI 534 TGATCA T^GATCA
FokI 1005 GGATG GGATG(9/13)
HaeII 100 GGCGCC [GA]GCGC^[CT]
HapII 31 CCGG C^CGG
Hin1I 100 GGCGCC G[GA]^CG[CT]C
HpaII 31 CCGG C^CGG
KasI 100 GGCGCC G^GCGCC
KspI 42 CCGCGG CCGC^GG
MluNI 563 TGGCCA TGG^CCA
MscI 563 TGGCCA TGG^CCA
MspI 31 CCGG C^CGG
NarI 100 GGCGCC GG^CGCC
NciI 30 CCCGG CC^[GC]GG
NcoI 820 CCATGG C^CATGG
PleI 847 GACTC GAGTC(4/5)
PpuMI 314 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 430 AACGTT AA^CGTT
PvuII 706 CAGCTG CAG^CTG
SacII 42 CCGCGG CCGC^GG
SfcI 638 CTGTAG C^T[GA][CT]AG
SfoI 100 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 600 GAATTC [GA]^AATT[CT]
768 AAATTT
AocI 204 CCTGAGG CC^T[ACGT]AGG
1015 CCTAAGG
ApoI 600 GAATTC [GA]^AATT[CT]
768 AAATTT
AvaII 39 GGTCC G^G[AT]CC
315 GGACC
BanI 100 GGCGCC G^G[CT][GA]CC
816 GGTGCC
BbsI 348 GAAGAC GAAGAC(2/6)
748 GAAGAC
BbvI 465 GCTGC GCAGC(8/12)
644 GCTGC
BfaI 779 CTAG C^TAG
1055 CTAG
BpmI 584 CTCCAG CTGGAG(16/14)
937 CTGGAG
BpuAI 348 GAAGAC GAAGAC(2/6)
748 GAAGAC
BspMI 461 ACCTGC ACCTGC(4/8)
48 GCAGGT
Bsu36I 204 CCTGAGG CC^T[ACGT]AGG
1015 CCTAAGG
DsaI 42 CCGCGG C^C[GA][CT]GG
820 CCATGG
EarI 797 CTCTTC CTCTTC(1/4)
1045 GAAGAG
Eco81I 204 CCTGAGG CC^T[ACGT]AGG
1015 CCTAAGG
EcoT14I 605 CCAAGG C^C[AT][AT]GG
820 CCATGG
HincII 136 GTCAAC GT[CT]^[GA]AC
879 GTTGAC
HindII 136 GTCAAC GT[CT]^[GA]AC
879 GTTGAC
Ksp632I 797 CTCTTC CTCTTC(1/4)
1045 GAAGAG
MaeI 779 CTAG C^TAG
1055 CTAG
MseI 409 TTAA T^TAA
772 TTAA
MspA1I 42 CCGCGG C[AC]G^C[GT]G
706 CAGCTG
PflMI 566 CCACACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
714 CCACATTTTGG
SmlI 448 CTCAAG C^T[CT][GA]AG
487 CTCAAG
StyI 605 CCAAGG C^C[AT][AT]GG
820 CCATGG
TfiI 254 GAATC G^A[AT]TC
1058 GATTC
Tru9I 409 TTAA T^TAA
772 TTAA
TseI 465 GCTGC G^C[AT]GC
644 GCTGC
Van91I 566 CCACACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
714 CCACATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BanII BglII BmyI BsaBI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI MaeII MaeIII MamI
MboI MboII MflI MnlI MvaI
MvnI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
Tsp45I Tsp509I TthHB8I XhoII