Restriction map for
pF1KB4219
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BciVI BfrI BglI BlnI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BsrBI BssHII BssSI BstBI
BstEII BstPI Bsu36I ClaI CpoI
DraI DraIII Eco47III Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI MfeI MluI MroI MseI
MunI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI Tru9I Tth111I
XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1225 GACGTC GACGT^C
AhdI 904 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 490 GTGCAC G^TGCAC
ApaLI 490 GTGCAC G^TGCAC
Asp700I 1203 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 904 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1402 CACGTG CAC^GTG
BclI 2238 TGATCA T^GATCA
BlpI 64 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 64 GCTGAGC GC^T[ACGT]AGC
BsaAI 1402 CACGTG [CT]AC^GT[GA]
BsgI 571 GTGCAG GTGCAG(16/14)
BsmBI 1227 CGTCTC CGTCTC(1/5)
Bsp1407I 945 TGTACA T^GTACA
BsrGI 945 TGTACA T^GTACA
Bst1107I 1743 GTATAC GTA^TAC
BstZ17I 1743 GTATAC GTA^TAC
CelII 64 GCTGAGC GC^T[ACGT]AGC
EagI 1379 CGGCCG C^GGCCG
Eam1105I 904 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1379 CGGCCG C^GGCCG
Eco52I 1379 CGGCCG C^GGCCG
EcoRI 2485 GAATTC G^AATTC
FbaI 2238 TGATCA T^GATCA
NdeI 1442 CATATG CA^TATG
NheI 447 GCTAGC G^CTAGC
PaeR7I 2041 CTCGAG C^TCGAG
PmaCI 1402 CACGTG CAC^GTG
PmlI 1402 CACGTG CAC^GTG
SacI 2026 GAGCTC GAGCT^C
SapI 1183 GCTCTTC GCTCTTC(1/4)
SexAI 1022 ACCAGGT A^CC[AT]GGT
SmaI 770 CCCGGG CCC^GGG
SspBI 945 TGTACA T^GTACA
Tsp45I 2420 GTGAC ^GT[GC]AC
XcmI 1016 CCACTGACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2041 CTCGAG C^TCGAG
XmaCI 770 CCCGGG C^CCGGG
XmaI 770 CCCGGG C^CCGGG
XmnI 1203 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 241 GTCTAC GT^[AC][GT]AC
1743 GTATAC
AcsI 1878 GAATTT [GA]^AATT[CT]
2485 GAATTC
AcyI 145 GACGCC G[GA]^CG[CT]C
1225 GACGTC
AflIII 1401 ACACGT A^C[GA][CT]GT
1841 ACACGT
ApaI 592 GGGCCC GGGCC^C
2786 GGGCCC
ApoI 1878 GAATTT [GA]^AATT[CT]
2485 GAATTC
BamHI 1766 GGATCC G^GATCC
1953 GGATCC
BanI 2183 GGCACC G^G[CT][GA]CC
2763 GGTGCC
BbvCI 1269 CCTCAGC CCTCAGC(-5/-2)
2350 GCTGAGG
BglII 1283 AGATCT A^GATCT
2054 AGATCT
BsaBI 1368 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2601 GATGCCCATC
BsaHI 145 GACGCC G[GA]^CG[CT]C
1225 GACGTC
BspHI 2085 TCATGA T^CATGA
2408 TCATGA
BsrDI 2595 CATTGC GCAATG(2/0)
356 GCAATG
BsrFI 60 ACCGGC [GA]^CCGG[CT]
1483 ACCGGC
BstAPI 573 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
981 GCAGGCCATGC
Cfr10I 60 ACCGGC [GA]^CCGG[CT]
1483 ACCGGC
DrdI 1225 GACGTCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2685 GACCAAGGTGTC
HgaI 145 GACGC GACGC(5/10)
1438 GACGC
Hin1I 145 GACGCC G[GA]^CG[CT]C
1225 GACGTC
MaeIII 2420 GTGAC ^GT[ACGT]AC
2726 GTAAC
MamI 1368 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2601 GATGCCCATC
RcaI 2085 TCATGA T^CATGA
2408 TCATGA
SbfI 138 CCTGCAGG CCTGCA^GG
528 CCTGCAGG
Sse8387I 138 CCTGCAGG CCTGCA^GG
528 CCTGCAGG
StuI 183 AGGCCT AGG^CCT
2555 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TseI Tsp509I TspRI TthHB8I Van91I
XhoII