Restriction map for
pF1KB4165
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# NO CUT ENZYMES ( 84 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tru9I
Tsp509I Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2116 GACGTC GACGT^C
AflIII 1511 ACGTGT A^C[GA][CT]GT
AhdI 544 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1069 GTGCAC G^TGCAC
Aor51HI 1717 AGCGCT AGC^GCT
ApaLI 1069 GTGCAC G^TGCAC
AspEI 544 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1477 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1884 TGGCCA TGG^CCA
BamHI 2446 GGATCC G^GATCC
BbrPI 2149 CACGTG CAC^GTG
BcgI 723 CGAGACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1727 AGATCT A^GATCT
BsaAI 2149 CACGTG [CT]AC^GT[GA]
BsaI 724 GAGACC GGTCTC(1/5)
BsiWI 1577 CGTACG C^GTACG
BsmBI 2260 GAGACG CGTCTC(1/5)
BstEII 1536 GGTCACC G^GT[ACGT]ACC
BstPI 1536 GGTCACC G^GT[ACGT]ACC
EagI 125 CGGCCG C^GGCCG
Eam1105I 544 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 125 CGGCCG C^GGCCG
Eco47III 1717 AGCGCT AGC^GCT
Eco52I 125 CGGCCG C^GGCCG
EcoO65I 1536 GGTCACC G^GT[ACGT]ACC
EcoT14I 972 CCATGG C^C[AT][AT]GG
HincII 1240 GTCAAC GT[CT]^[GA]AC
HindII 1240 GTCAAC GT[CT]^[GA]AC
MluNI 1884 TGGCCA TGG^CCA
MscI 1884 TGGCCA TGG^CCA
MslI 1335 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 972 CCATGG C^CATGG
NspI 587 GCATGC [GA]CATG^[CT]
PflFI 1477 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2149 CACGTG CAC^GTG
PmlI 2149 CACGTG CAC^GTG
SapI 1662 GCTCTTC GCTCTTC(1/4)
SexAI 2012 ACCTGGT A^CC[AT]GGT
SmaI 153 CCCGGG CCC^GGG
SphI 587 GCATGC GCATG^C
StyI 972 CCATGG C^C[AT][AT]GG
Tth111I 1477 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2361 TCTAGA T^CTAGA
XcmI 1043 CCAGAGCCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 153 CCCGGG C^CCGGG
XmaI 153 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 1091 TCCGGA T^CCGGA
2453 TCCGGA
AvaI 82 CCCGAG C^[CT]CG[GA]G
153 CCCGGG
AviII 479 TGCGCA TGC^GCA
1265 TGCGCA
BbeI 704 GGCGCC GGCGC^C
1591 GGCGCC
BbsI 621 GAAGAC GAAGAC(2/6)
1483 GTCTTC
BbvCI 813 CCTCAGC CCTCAGC(-5/-2)
637 GCTGAGG
BpuAI 621 GAAGAC GAAGAC(2/6)
1483 GTCTTC
BsaWI 1091 TCCGGA [AT]^CCGG[AT]
2453 TCCGGA
BseAI 1091 TCCGGA T^CCGGA
2453 TCCGGA
BsmAI 724 GAGAC GTCTC(1/5)
2260 GAGAC
BsoBI 82 CCCGAG C^[CT]CG[GA]G
153 CCCGGG
BspEI 1091 TCCGGA T^CCGGA
2453 TCCGGA
BsrDI 729 CATTGC GCAATG(2/0)
2121 CATTGC
EarI 1663 CTCTTC CTCTTC(1/4)
1006 GAAGAG
FspI 479 TGCGCA TGC^GCA
1265 TGCGCA
KasI 704 GGCGCC G^GCGCC
1591 GGCGCC
Ksp632I 1663 CTCTTC CTCTTC(1/4)
1006 GAAGAG
MroI 1091 TCCGGA T^CCGGA
2453 TCCGGA
NarI 704 GGCGCC GG^CGCC
1591 GGCGCC
SacI 903 GAGCTC GAGCT^C
1312 GAGCTC
SbfI 309 CCTGCAGG CCTGCA^GG
843 CCTGCAGG
SfoI 704 GGCGCC GGC^GCC
1591 GGCGCC
Sse8387I 309 CCTGCAGG CCTGCA^GG
843 CCTGCAGG
TfiI 1077 GAATC G^A[AT]TC
2191 GATTC
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI TaqI TseI
Tsp45I TspRI TthHB8I XhoII