Restriction map for
pF1KB4108
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccII AccIII AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BglI BglII BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI FauI FseI FspI HaeII
HhaI Hin1I HinP1I KasI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 2000 GGTACC G^GTACC
AccI 2725 GTAGAC GT^[AC][GT]AC
AclI 1686 AACGTT AA^CGTT
AflII 267 CTTAAG C^TTAAG
AseI 187 ATTAAT AT^TAAT
AsnI 187 ATTAAT AT^TAAT
Asp700I 796 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2000 GGTACC G^GTACC
AspHI 250 GAGCTC G[AT]GC[AT]^C
AspI 2893 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 2006 TGATCA T^GATCA
BcnI 2698 CCCGG CC^[GC]GG
BfrI 267 CTTAAG C^TTAAG
BlpI 2569 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2569 GCTGAGC GC^T[ACGT]AGC
BsaAI 2914 TACGTG [CT]AC^GT[GA]
BsiHKAI 250 GAGCTC G[AT]GC[AT]^C
BspMI 1642 ACCTGC ACCTGC(4/8)
BstAPI 2422 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2683 GGTGACC G^GT[ACGT]ACC
BstPI 2683 GGTGACC G^GT[ACGT]ACC
CelII 2569 GCTGAGC GC^T[ACGT]AGC
DraI 175 TTTAAA TTT^AAA
DraIII 2678 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1698 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1222 CCGTGG C^C[GA][CT]GG
EcoO65I 2683 GGTGACC G^GT[ACGT]ACC
EcoRV 2521 GATATC GAT^ATC
EcoT14I 605 CCTTGG C^C[AT][AT]GG
EcoT22I 1676 ATGCAT ATGCA^T
FbaI 2006 TGATCA T^GATCA
HapII 2699 CCGG C^CGG
HgaI 1470 GCGTC GACGC(5/10)
HpaI 1638 GTTAAC GTT^AAC
HpaII 2699 CCGG C^CGG
KpnI 2000 GGTACC GGTAC^C
MspI 2699 CCGG C^CGG
NciI 2698 CCCGG CC^[GC]GG
NdeI 1239 CATATG CA^TATG
NheI 1581 GCTAGC G^CTAGC
NsiI 1676 ATGCAT ATGCA^T
PaeR7I 2663 CTCGAG C^TCGAG
PflFI 2893 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 187 ATTAAT AT^TAAT
Psp1406I 1686 AACGTT AA^CGTT
PvuII 922 CAGCTG CAG^CTG
SacI 250 GAGCTC GAGCT^C
SapI 724 GAAGAGC GCTCTTC(1/4)
SexAI 2135 ACCTGGT A^CC[AT]GGT
StyI 605 CCTTGG C^C[AT][AT]GG
Tth111I 2893 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2663 CTCGAG C^TCGAG
XmnI 796 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 1980 CCCGAG C^[CT]CG[GA]G
2663 CTCGAG
BalI 608 TGGCCA TGG^CCA
872 TGGCCA
BsaI 44 GAGACC GGTCTC(1/5)
2701 GGTCTC
BseRI 220 CTCCTC GAGGAG(10/8)
963 GAGGAG
BsiYI 1709 CCCTGTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1796 CCATGTTTGGG
BslI 1709 CCCTGTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1796 CCATGTTTGGG
BsoBI 1980 CCCGAG C^[CT]CG[GA]G
2663 CTCGAG
BssSI 514 CACGAG CACGAG(-5/-1)
1391 CACGAG
BstXI 785 CCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1418 CCACTCTGGTGG
BstYI 642 AGATCC [GA]^GATC[CT]
2015 GGATCT
EaeI 608 TGGCCA [CT]^GGCC[GA]
872 TGGCCA
EcoRI 412 GAATTC G^AATTC
706 GAATTC
HindIII 270 AAGCTT A^AGCTT
1921 AAGCTT
MflI 642 AGATCC [GA]^GATC[CT]
2015 GGATCT
MluNI 608 TGGCCA TGG^CCA
872 TGGCCA
MscI 608 TGGCCA TGG^CCA
872 TGGCCA
MspA1I 522 CCGCTG C[AC]G^C[GT]G
922 CAGCTG
PshAI 820 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1749 GACCAATGTC
PstI 1702 CTGCAG CTGCA^G
2627 CTGCAG
StuI 602 AGGCCT AGG^CCT
2187 AGGCCT
XcmI 548 CCAGTTCAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1390 CCACGAGAATCATGG
XhoII 642 AGATCC [GA]^GATC[CT]
2015 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MnlI MseI MslI MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I