Restriction map for
pF1KB4084
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII BbeI BfrI BsiEI
BsmI Bsp1407I BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstZ17I
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HaeII KasI KpnI KspI
MfeI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 881 TCCGGA T^CCGGA
AcyI 1702 GACGCC G[GA]^CG[CT]C
Asp700I 1365 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2701 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 253 CCTAGG C^CTAGG
BanI 224 GGCACC G^G[CT][GA]CC
BbvCI 748 GCTGAGG CCTCAGC(-5/-2)
BciVI 2084 GGATAC GTATCC(6/5)
BclI 1497 TGATCA T^GATCA
BglI 814 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1805 AGATCT A^GATCT
BlnI 253 CCTAGG C^CTAGG
BlpI 1674 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1674 GCTGAGC GC^T[ACGT]AGC
BsaBI 972 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1702 GACGCC G[GA]^CG[CT]C
BsaWI 881 TCCGGA [AT]^CCGG[AT]
BseAI 881 TCCGGA T^CCGGA
BsiWI 2052 CGTACG C^GTACG
BspDI 557 ATCGAT AT^CGAT
BspEI 881 TCCGGA T^CCGGA
BssHII 2838 GCGCGC G^CGCGC
BstAPI 2466 GCATAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 900 GGTGACC G^GT[ACGT]ACC
BstPI 900 GGTGACC G^GT[ACGT]ACC
CelII 1674 GCTGAGC GC^T[ACGT]AGC
ClaI 557 ATCGAT AT^CGAT
DraIII 675 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 900 GGTGACC G^GT[ACGT]ACC
FbaI 1497 TGATCA T^GATCA
Hin1I 1702 GACGCC G[GA]^CG[CT]C
HindIII 1438 AAGCTT A^AGCTT
HpaI 1377 GTTAAC GTT^AAC
MamI 972 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1275 ACGCGT A^CGCGT
MroI 881 TCCGGA T^CCGGA
NaeI 1171 GCCGGC GCC^GGC
NgoMIV 1171 GCCGGC G^CCGGC
PaeR7I 2546 CTCGAG C^TCGAG
PflFI 2701 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 65 CTGCAG CTGCA^G
ScaI 2609 AGTACT AGT^ACT
SmlI 2546 CTCGAG C^T[CT][GA]AG
Tth111I 2701 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1455 TCTAGA T^CTAGA
XhoI 2546 CTCGAG C^TCGAG
XmnI 1365 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 1276 CGCG CG^CG
2839 CGCG
AhdI 280 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1532 GACAGAGAGTC
AlwNI 119 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
862 CAGCGACTG
AocI 88 CCTCAGG CC^T[ACGT]AGG
435 CCTGAGG
ApaI 812 GGGCCC GGGCC^C
2800 GGGCCC
AspEI 280 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1532 GACAGAGAGTC
BamHI 696 GGATCC G^GATCC
2141 GGATCC
BbrPI 442 CACGTG CAC^GTG
1501 CACGTG
BcgI 318 CGACCAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1875 GCAGAGTTTTCG
BsaAI 442 CACGTG [CT]AC^GT[GA]
1501 CACGTG
BsmBI 287 CGTCTC CGTCTC(1/5)
2560 CGTCTC
BsrFI 191 GCCGGT [GA]^CCGG[CT]
1171 GCCGGC
BsrI 1177 ACTGG ACTGG(1/-1)
1347 CCAGT
BstUI 1276 CGCG CG^CG
2839 CGCG
Bsu36I 88 CCTCAGG CC^T[ACGT]AGG
435 CCTGAGG
Cfr10I 191 GCCGGT [GA]^CCGG[CT]
1171 GCCGGC
Eam1105I 280 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1532 GACAGAGAGTC
Eco81I 88 CCTCAGG CC^T[ACGT]AGG
435 CCTGAGG
EcoNI 88 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
738 CCTCCCCGAGG
EcoRI 1029 GAATTC G^AATTC
2071 GAATTC
MvnI 1276 CGCG CG^CG
2839 CGCG
NcoI 1316 CCATGG C^CATGG
2843 CCATGG
NspI 1601 ACATGC [GA]CATG^[CT]
2427 GCATGT
PflMI 188 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1311 CCACTCCATGG
PmaCI 442 CACGTG CAC^GTG
1501 CACGTG
PmlI 442 CACGTG CAC^GTG
1501 CACGTG
PshAI 28 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
334 GACGTGGGTC
SfcI 65 CTGCAG C^T[GA][CT]AG
2736 CTATAG
StuI 250 AGGCCT AGG^CCT
2406 AGGCCT
TaqI 558 TCGA T^CGA
2547 TCGA
TthHB8I 558 TCGA T^CGA
2547 TCGA
Van91I 188 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1311 CCACTCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII