Restriction map for
pF1KB4018
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
FbaI FseI FspI HaeII HgaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI NspV PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tsp45I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 85 GACGTC GACGT^C
AccI 88 GTCTAC GT^[AC][GT]AC
AcyI 85 GACGTC G[GA]^CG[CT]C
AflII 1023 CTTAAG C^TTAAG
AflIII 1221 ACACGT A^C[GA][CT]GT
AocI 1469 CCTTAGG CC^T[ACGT]AGG
Asp700I 504 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 316 GGACC G^G[AT]CC
BalI 999 TGGCCA TGG^CCA
BcnI 7 CCCGG CC^[GC]GG
BfrI 1023 CTTAAG C^TTAAG
BsaAI 307 CACGTA [CT]AC^GT[GA]
BsaHI 85 GACGTC G[GA]^CG[CT]C
BsmBI 2263 GAGACG CGTCTC(1/5)
BstXI 1494 CCAGTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1469 CCTTAGG CC^T[ACGT]AGG
CfoI 60 GCGC GCG^C
DraIII 742 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 999 TGGCCA [CT]^GGCC[GA]
EarI 2286 CTCTTC CTCTTC(1/4)
Eco81I 1469 CCTTAGG CC^T[ACGT]AGG
EcoRI 941 GAATTC G^AATTC
EcoT22I 1342 ATGCAT ATGCA^T
HhaI 60 GCGC GCG^C
Hin1I 85 GACGTC G[GA]^CG[CT]C
HinP1I 60 GCGC G^CGC
HpaI 106 GTTAAC GTT^AAC
Ksp632I 2286 CTCTTC CTCTTC(1/4)
MluNI 999 TGGCCA TGG^CCA
MscI 999 TGGCCA TGG^CCA
NciI 7 CCCGG CC^[GC]GG
NheI 2490 GCTAGC G^CTAGC
NsiI 1342 ATGCAT ATGCA^T
NspI 1636 GCATGT [GA]CATG^[CT]
PacI 2128 TTAATTAA TTAAT^TAA
PleI 1179 GAGTC GAGTC(4/5)
PshAI 317 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 361 CTGCAG CTGCA^G
PvuII 548 CAGCTG CAG^CTG
SacI 1485 GAGCTC GAGCT^C
ScaI 1718 AGTACT AGT^ACT
SfcI 361 CTGCAG C^T[GA][CT]AG
SspI 214 AATATT AAT^ATT
XmnI 504 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 1485 GAGCTC G[AT]GC[AT]^C
2069 GAGCAC
AspI 831 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2593 GACAGGGTC
BanII 1398 GAGCCC G[GA]GC[CT]^C
1485 GAGCTC
BglII 1108 AGATCT A^GATCT
2530 AGATCT
BpmI 536 CTCCAG CTGGAG(16/14)
2344 CTGGAG
BsgI 473 CTGCAC GTGCAG(16/14)
172 GTGCAG
BsiHKAI 1485 GAGCTC G[AT]GC[AT]^C
2069 GAGCAC
BsmI 2510 GAATGC GAATGC(1/-1)
1344 GCATTC
Bsp1407I 1810 TGTACA T^GTACA
2651 TGTACA
BsrGI 1810 TGTACA T^GTACA
2651 TGTACA
Cac8I 1838 GCTTGC GC[ACGT]^[ACGT]GC
2490 GCTAGC
Cfr13I 316 GGACC G^G[ACGT]CC
2049 GGGCC
HapII 8 CCGG C^CGG
27 CCGG
HincII 106 GTTAAC GT[CT]^[GA]AC
1526 GTCAAC
HindII 106 GTTAAC GT[CT]^[GA]AC
1526 GTCAAC
HindIII 253 AAGCTT A^AGCTT
1859 AAGCTT
HpaII 8 CCGG C^CGG
27 CCGG
MfeI 491 CAATTG C^AATTG
1833 CAATTG
MspA1I 69 CAGCGG C[AC]G^C[GT]G
548 CAGCTG
MspI 8 CCGG C^CGG
27 CCGG
MunI 491 CAATTG C^AATTG
1833 CAATTG
NdeI 341 CATATG CA^TATG
1627 CATATG
PflFI 831 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2593 GACAGGGTC
Sau96I 316 GGACC G^G[ACGT]CC
2049 GGGCC
SmlI 1023 CTTAAG C^T[CT][GA]AG
1186 CTCAAG
SspBI 1810 TGTACA T^GTACA
2651 TGTACA
Tth111I 831 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2593 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanI BbsI BbvI BfaI
BmyI BpuAI BsaBI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
DdeI DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV RcaI RsaI Sau3AI ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII