Restriction map for
pF1KB4014
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI StyI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 927 GGTACC G^GTACC
AcsI 1249 AAATTT [GA]^AATT[CT]
AcyI 1313 GGCGCC G[GA]^CG[CT]C
AlwI 593 GATCC GGATC(4/5)
ApoI 1249 AAATTT [GA]^AATT[CT]
Asp718I 927 GGTACC G^GTACC
BbeI 1313 GGCGCC GGCGC^C
BglI 971 GCCCGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1313 GGCGCC G[GA]^CG[CT]C
BsaWI 960 TCCGGT [AT]^CCGG[AT]
BsmBI 676 GAGACG CGTCTC(1/5)
Bsp1407I 315 TGTACA T^GTACA
BspHI 1280 TCATGA T^CATGA
BsrGI 315 TGTACA T^GTACA
BssSI 1134 CACGAG CACGAG(-5/-1)
BstYI 592 AGATCC [GA]^GATC[CT]
Cfr13I 1128 GGCCC G^G[ACGT]CC
DpnII 593 GATC ^GATC
DraI 1108 TTTAAA TTT^AAA
EarI 187 GAAGAG CTCTTC(1/4)
HgaI 1140 GACGC GACGC(5/10)
Hin1I 1313 GGCGCC G[GA]^CG[CT]C
KasI 1313 GGCGCC G^GCGCC
KpnI 927 GGTACC GGTAC^C
Ksp632I 187 GAAGAG CTCTTC(1/4)
MaeII 143 ACGT A^CGT
MboI 593 GATC ^GATC
MflI 592 AGATCC [GA]^GATC[CT]
NarI 1313 GGCGCC GG^CGCC
NdeII 593 GATC ^GATC
NspI 1305 GCATGC [GA]CATG^[CT]
PflMI 1087 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 825 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1280 TCATGA T^CATGA
Sau3AI 593 GATC ^GATC
Sau96I 1128 GGCCC G^G[ACGT]CC
SfoI 1313 GGCGCC GGC^GCC
SmlI 278 CTCAAG C^T[CT][GA]AG
SphI 1305 GCATGC GCATG^C
SspBI 315 TGTACA T^GTACA
Van91I 1087 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1083 CCACCCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 592 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 316 GTAC GT^AC
928 GTAC
BanII 384 GAGCCC G[GA]GC[CT]^C
969 GAGCCC
BcnI 202 CCGGG CC^[GC]GG
1310 CCCGG
BsaI 692 GGTCTC GGTCTC(1/5)
735 GGTCTC
BsaJI 1009 CCTGGG C^C[ACGT][ACGT]GG
1086 CCCAGG
BsgI 644 CTGCAC GTGCAG(16/14)
943 GTGCAG
BsmFI 711 GTCCC GGGAC(10/14)
901 GTCCC
BsrFI 777 GCCGGT [GA]^CCGG[CT]
1271 ACCGGC
BstAPI 637 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
748 GCAGTATCTGC
BstF5I 495 CATCC GGATG(2/0)
1090 GGATG
Cfr10I 777 GCCGGT [GA]^CCGG[CT]
1271 ACCGGC
FokI 495 CATCC GGATG(9/13)
1090 GGATG
HaeIII 32 GGCC GG^CC
1128 GGCC
MseI 134 TTAA T^TAA
1109 TTAA
NciI 202 CCGGG CC^[GC]GG
1310 CCCGG
PvuII 443 CAGCTG CAG^CTG
1164 CAGCTG
RsaI 316 GTAC GT^AC
928 GTAC
SfaNI 448 GATGC GCATC(5/9)
494 GCATC
Tru9I 134 TTAA T^TAA
1109 TTAA
Tsp509I 514 AATT ^AATT
1250 AATT
TspRI 614 CAGTG CA[GC]TG[ACGT][ACGT]^
728 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwNI BanI BbvI
BmyI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BsrDI BsrI BssKI
BstNI Cac8I CfoI DdeI EcoRII
Fnu4HI HaeII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NlaIII NlaIV PleI
PstI ScrFI SfcI TfiI TseI
Tsp45I