Restriction map for
pF1KB3975
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BbrPI BbsI BciVI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsiWI BsmI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI ClaI CpoI DraI DraIII
DrdI EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KpnI MaeI
MaeII MamI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SapI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 884 ACATGT A^C[GA][CT]GT
AlwI 806 GGATC GGATC(4/5)
AocI 844 CCTGAGG CC^T[ACGT]AGG
BcgI 1876 GCAGCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 387 TGATCA T^GATCA
BlpI 696 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 696 GCTCAGC GC^T[ACGT]AGC
BseRI 1425 CTCCTC GAGGAG(10/8)
BsmAI 625 GTCTC GTCTC(1/5)
BsmBI 624 CGTCTC CGTCTC(1/5)
BspLU11I 884 ACATGT A^CATGT
BsrBI 837 CCGCTC CCGCTC(-3/-3)
Bst1107I 184 GTATAC GTA^TAC
BstYI 806 GGATCT [GA]^GATC[CT]
BstZ17I 184 GTATAC GTA^TAC
Bsu36I 844 CCTGAGG CC^T[ACGT]AGG
CelII 696 GCTCAGC GC^T[ACGT]AGC
Eco81I 844 CCTGAGG CC^T[ACGT]AGG
EcoNI 444 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 387 TGATCA T^GATCA
HgaI 623 GCGTC GACGC(5/10)
HincII 955 GTCGAC GT[CT]^[GA]AC
HindII 955 GTCGAC GT[CT]^[GA]AC
KspI 618 CCGCGG CCGC^GG
MflI 806 GGATCT [GA]^GATC[CT]
NaeI 1163 GCCGGC GCC^GGC
NgoMIV 1163 GCCGGC G^CCGGC
PflMI 1212 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 694 GAGCTC GAGCT^C
SacII 618 CCGCGG CCGC^GG
SalI 955 GTCGAC G^TCGAC
SexAI 1217 ACCTGGT A^CC[AT]GGT
SfiI 1302 GGCCGACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 718 CTCAAG C^T[CT][GA]AG
SphI 1229 GCATGC GCATG^C
TfiI 232 GATTC G^A[AT]TC
Tsp509I 336 AATT ^AATT
Van91I 1212 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 761 CCATCAAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 806 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 184 GTATAC GT^[AC][GT]AC
955 GTCGAC
AccII 619 CGCG CG^CG
1356 CGCG
AccIII 545 TCCGGA T^CCGGA
1775 TCCGGA
AhdI 883 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1788 GACTGGAGGTC
Aor51HI 314 AGCGCT AGC^GCT
326 AGCGCT
AspEI 883 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1788 GACTGGAGGTC
AvaI 1108 CCCGGG C^[CT]CG[GA]G
1156 CCCGGG
BanI 219 GGCGCC G^G[CT][GA]CC
1017 GGCGCC
BbeI 219 GGCGCC GGCGC^C
1017 GGCGCC
BbvCI 122 CCTCAGC CCTCAGC(-5/-2)
1766 CCTCAGC
BseAI 545 TCCGGA T^CCGGA
1775 TCCGGA
BsgI 663 CTGCAC GTGCAG(16/14)
1141 GTGCAG
BsiEI 998 CGGCCG CG[GA][CT]^CG
1301 CGGCCG
BsoBI 1108 CCCGGG C^[CT]CG[GA]G
1156 CCCGGG
BspEI 545 TCCGGA T^CCGGA
1775 TCCGGA
BspMI 1369 ACCTGC ACCTGC(4/8)
60 GCAGGT
BsrFI 996 ACCGGC [GA]^CCGG[CT]
1163 GCCGGC
BstUI 619 CGCG CG^CG
1356 CGCG
Cfr10I 996 ACCGGC [GA]^CCGG[CT]
1163 GCCGGC
EagI 998 CGGCCG C^GGCCG
1301 CGGCCG
Eam1105I 883 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1788 GACTGGAGGTC
EarI 934 CTCTTC CTCTTC(1/4)
1377 CTCTTC
EclXI 998 CGGCCG C^GGCCG
1301 CGGCCG
Eco47III 314 AGCGCT AGC^GCT
326 AGCGCT
Eco52I 998 CGGCCG C^GGCCG
1301 CGGCCG
KasI 219 GGCGCC G^GCGCC
1017 GGCGCC
Ksp632I 934 CTCTTC CTCTTC(1/4)
1377 CTCTTC
MroI 545 TCCGGA T^CCGGA
1775 TCCGGA
MvnI 619 CGCG CG^CG
1356 CGCG
NarI 219 GGCGCC GG^CGCC
1017 GGCGCC
NcoI 18 CCATGG C^CATGG
267 CCATGG
NspI 884 ACATGT [GA]CATG^[CT]
1229 GCATGC
PleI 340 GACTC GAGTC(4/5)
1779 GACTC
PstI 1123 CTGCAG CTGCA^G
1276 CTGCAG
ScaI 509 AGTACT AGT^ACT
1034 AGTACT
SfoI 219 GGCGCC GGC^GCC
1017 GGCGCC
SmaI 1108 CCCGGG CCC^GGG
1156 CCCGGG
Tsp45I 1433 GTGAC ^GT[GC]AC
1599 GTCAC
XmaCI 1108 CCCGGG C^CCGGG
1156 CCCGGG
XmaI 1108 CCCGGG C^CCGGG
1156 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI AvaII AviII BalI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI TspRI
TthHB8I