Restriction map for
pF1KB3935
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MfeI
MflI MluI MroI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SmaI
SpeI SphI SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 642 GGTACC G^GTACC
AlwNI 472 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 642 GGTACC G^GTACC
BanI 642 GGTACC G^G[CT][GA]CC
BcgI 56 CGACTGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 673 CTAG C^TAG
BglI 1246 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 525 GGGCAC G[AGT]GC[ACT]^C
BsaBI 691 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1066 GTGCAG GTGCAG(16/14)
BsmAI 583 GAGAC GTCTC(1/5)
BsmFI 736 GTCCC GGGAC(10/14)
BsmI 297 GAATGC GAATGC(1/-1)
Bsp1286I 525 GGGCAC G[AGT]GC[ACT]^C
BsrFI 1150 ACCGGC [GA]^CCGG[CT]
BstAPI 1215 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1154 GCGC GCG^C
Cfr10I 1150 ACCGGC [GA]^CCGG[CT]
DraI 730 TTTAAA TTT^AAA
DsaI 172 CCGTGG C^C[GA][CT]GG
EcoNI 94 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 826 GATATC GAT^ATC
EcoT14I 512 CCTTGG C^C[AT][AT]GG
EcoT22I 113 ATGCAT ATGCA^T
HapII 1151 CCGG C^CGG
HhaI 1154 GCGC GCG^C
HinP1I 1154 GCGC G^CGC
HpaII 1151 CCGG C^CGG
KpnI 642 GGTACC GGTAC^C
MaeI 673 CTAG C^TAG
MamI 691 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 303 CATTAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 472 CAGCTG C[AC]G^C[GT]G
MspI 1151 CCGG C^CGG
NlaIV 642 GGTACC GG[ACGT]^[ACGT]CC
NruI 53 TCGCGA TCG^CGA
NsiI 113 ATGCAT ATGCA^T
NspI 1178 ACATGC [GA]CATG^[CT]
PflMI 471 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1222 CTGCAG CTGCA^G
PvuII 472 CAGCTG CAG^CTG
SapI 805 GAAGAGC GCTCTTC(1/4)
SbfI 1221 CCTGCAGG CCTGCA^GG
SfcI 1222 CTGCAG C^T[GA][CT]AG
SgrAI 1149 CACCGGCG C[GA]^CCGG[CT]G
SmlI 355 CTCAAG C^T[CT][GA]AG
SnaBI 847 TACGTA TAC^GTA
Sse8387I 1221 CCTGCAGG CCTGCA^GG
StyI 512 CCTTGG C^C[AT][AT]GG
TfiI 942 GATTC G^A[AT]TC
Van91I 471 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 163 CCATTGCCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 54 CGCG CG^CG
1155 CGCG
BalI 759 TGGCCA TGG^CCA
1184 TGGCCA
BbsI 333 GAAGAC GAAGAC(2/6)
616 GTCTTC
BpmI 95 CTGGAG CTGGAG(16/14)
883 CTGGAG
BpuAI 333 GAAGAC GAAGAC(2/6)
616 GTCTTC
BsaAI 541 TACGTG [CT]AC^GT[GA]
847 TACGTA
BseRI 418 GAGGAG GAGGAG(10/8)
1231 GAGGAG
BsrDI 164 CATTGC GCAATG(2/0)
71 GCAATG
BstF5I 360 GGATG GGATG(2/0)
909 GGATG
BstUI 54 CGCG CG^CG
1155 CGCG
DraII 23 AGGCCCT [GA]G^G[ACGT]CC[CT]
350 AGGCCCT
EarI 415 GAAGAG CTCTTC(1/4)
805 GAAGAG
EcoO109I 23 AGGCCCT [GA]G^G[ACGT]CC[CT]
350 AGGCCCT
FauI 36 CCCGC CCCGC(4/6)
508 CCCGC
FokI 360 GGATG GGATG(9/13)
909 GGATG
Ksp632I 415 GAAGAG CTCTTC(1/4)
805 GAAGAG
MaeII 542 ACGT A^CGT
848 ACGT
MluNI 759 TGGCCA TGG^CCA
1184 TGGCCA
MscI 759 TGGCCA TGG^CCA
1184 TGGCCA
MvnI 54 CGCG CG^CG
1155 CGCG
PleI 12 GAGTC GAGTC(4/5)
1024 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI BsiYI BslI
BspMI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII PshAI
RsaI Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI Tsp45I Tsp509I TspRI