Restriction map for
pF1KB3761
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AocI ApaI
ApaLI AscI Asp700I Asp718I AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BseAI BsiWI BsmBI
BsoBI BspEI BspHI BspMI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I CelII CpoI DrdI Eco81I
EcoO65I EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MroI MspA1I
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 393 GTCTAC GT^[AC][GT]AC
AhdI 1308 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2998 AGCGCT AGC^GCT
AseI 2271 ATTAAT AT^TAAT
AsnI 2271 ATTAAT AT^TAAT
AspEI 1308 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2114 TGGCCA TGG^CCA
BanII 1636 GGGCTC G[GA]GC[CT]^C
BbvCI 22 GCTGAGG CCTCAGC(-5/-2)
BcnI 50 CCGGG CC^[GC]GG
BglI 49 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1114 ACCGGA [AT]^CCGG[AT]
BsiEI 104 CGGCCG CG[GA][CT]^CG
BsmI 859 GAATGC GAATGC(1/-1)
Bsp1407I 3008 TGTACA T^GTACA
BspDI 3103 ATCGAT AT^CGAT
BsrBI 1623 CCGCTC CCGCTC(-3/-3)
BsrDI 744 CATTGC GCAATG(2/0)
BsrFI 1184 GCCGGT [GA]^CCGG[CT]
BsrGI 3008 TGTACA T^GTACA
BssSI 1010 CTCGTG CACGAG(-5/-1)
Cfr10I 1184 GCCGGT [GA]^CCGG[CT]
ClaI 3103 ATCGAT AT^CGAT
DraI 679 TTTAAA TTT^AAA
DraIII 1220 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 218 CCACGG C^C[GA][CT]GG
EagI 104 CGGCCG C^GGCCG
Eam1105I 1308 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 104 CGGCCG C^GGCCG
Eco47III 2998 AGCGCT AGC^GCT
Eco52I 104 CGGCCG C^GGCCG
EcoRI 2473 GAATTC G^AATTC
EcoRV 3046 GATATC GAT^ATC
EcoT14I 1558 CCTTGG C^C[AT][AT]GG
FauI 2964 CCCGC CCCGC(4/6)
HincII 1316 GTCAAC GT[CT]^[GA]AC
HindII 1316 GTCAAC GT[CT]^[GA]AC
MluI 3132 ACGCGT A^CGCGT
MluNI 2114 TGGCCA TGG^CCA
MscI 2114 TGGCCA TGG^CCA
MslI 2968 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 50 CCGGG CC^[GC]GG
PshBI 2271 ATTAAT AT^TAAT
SapI 727 GAAGAGC GCTCTTC(1/4)
SexAI 2091 ACCTGGT A^CC[AT]GGT
SmlI 1641 CTCAAG C^T[CT][GA]AG
SphI 196 GCATGC GCATG^C
SspBI 3008 TGTACA T^GTACA
StuI 2982 AGGCCT AGG^CCT
StyI 1558 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 102 CGCG CG^CG
3133 CGCG
AlwNI 64 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
76 CAGGAGCTG
AspHI 802 GAGCAC G[AT]GC[AT]^C
1525 GTGCTC
BcgI 120 CGACTTTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3105 CGATAGTGATGC
BpmI 1732 CTCCAG CTGGAG(16/14)
2186 CTGGAG
BsaAI 2408 TACGTG [CT]AC^GT[GA]
2629 CACGTA
BsgI 1715 CTGCAC GTGCAG(16/14)
1813 GTGCAG
BsiHKAI 802 GAGCAC G[AT]GC[AT]^C
1525 GTGCTC
BsmFI 1646 GGGAC GGGAC(10/14)
1818 GGGAC
BspLU11I 329 ACATGT A^CATGT
1460 ACATGT
BstAPI 1957 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2176 GCAAATGATGC
BstBI 1874 TTCGAA TT^CGAA
1895 TTCGAA
BstUI 102 CGCG CG^CG
3133 CGCG
Cac8I 196 GCATGC GC[ACGT]^[ACGT]GC
1386 GCTGGC
EaeI 104 CGGCCG [CT]^GGCC[GA]
2114 TGGCCA
HaeII 57 GGCGCT [GA]GCGC^[CT]
2998 AGCGCT
HgaI 973 GACGC GACGC(5/10)
3134 GCGTC
MvnI 102 CGCG CG^CG
3133 CGCG
NdeI 449 CATATG CA^TATG
2866 CATATG
NlaIV 1989 GGACCC GG[ACGT]^[ACGT]CC
2356 GGGTCC
NspV 1874 TTCGAA TT^CGAA
1895 TTCGAA
PflMI 2161 CCATTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2865 CCATATGATGG
PpuMI 1988 AGGACCC [GA]G^G[AT]CC[CT]
2356 GGGTCCT
SfuI 1874 TTCGAA TT^CGAA
1895 TTCGAA
SspI 402 AATATT AAT^ATT
1240 AATATT
Van91I 2161 CCATTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2865 CCATATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI CfoI
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII