Restriction map for
pF1KB3734
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 28 CGCG CG^CG
AvaI 1229 CTCGGG C^[CT]CG[GA]G
BamHI 96 GGATCC G^GATCC
BbvI 912 GCTGC GCAGC(8/12)
BcnI 351 CCGGG CC^[GC]GG
BglII 341 AGATCT A^GATCT
BsaBI 1084 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 217 GAGGAG GAGGAG(10/8)
BsmAI 963 GAGAC GTCTC(1/5)
BsmFI 253 GGGAC GGGAC(10/14)
BsmI 1135 GCATTC GAATGC(1/-1)
BsoBI 1229 CTCGGG C^[CT]CG[GA]G
BspHI 810 TCATGA T^CATGA
BspMI 1046 ACCTGC ACCTGC(4/8)
BstUI 28 CGCG CG^CG
DraII 253 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1207 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 395 CCGTGG C^C[GA][CT]GG
EaeI 1187 TGGCCG [CT]^GGCC[GA]
EcoO109I 253 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1084 GATATC GAT^ATC
EcoT14I 1307 CCTTGG C^C[AT][AT]GG
FauI 237 GCGGG CCCGC(4/6)
Fnu4HI 912 GCTGC GC^[ACGT]GC
HaeIII 1188 GGCC GG^CC
HapII 351 CCGG C^CGG
HpaII 351 CCGG C^CGG
ItaI 912 GCTGC GC^[ACGT]GC
MaeIII 689 GTCAC ^GT[ACGT]AC
MamI 1084 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 910 CAGCTG C[AC]G^C[GT]G
MspI 351 CCGG C^CGG
MvnI 28 CGCG CG^CG
NciI 351 CCGGG CC^[GC]GG
PleI 1324 GACTC GAGTC(4/5)
PpuMI 253 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 910 CAGCTG CAG^CTG
RcaI 810 TCATGA T^CATGA
SacI 403 GAGCTC GAGCT^C
SexAI 1069 ACCTGGT A^CC[AT]GGT
SfcI 1218 CTACAG C^T[GA][CT]AG
StyI 1307 CCTTGG C^C[AT][AT]GG
TseI 912 GCTGC G^C[AT]GC
Tsp45I 689 GTCAC ^GT[GC]AC
XbaI 828 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 403 GAGCTC G[AT]GC[AT]^C
1285 GTGCTC
BanII 403 GAGCTC G[GA]GC[CT]^C
1249 GAGCCC
BpmI 101 CTCCAG CTGGAG(16/14)
42 CTGGAG
BsiHKAI 403 GAGCTC G[AT]GC[AT]^C
1285 GTGCTC
Bsp1407I 2 TGTACA T^GTACA
1262 TGTACA
BsrGI 2 TGTACA T^GTACA
1262 TGTACA
BstNI 618 CCAGG CC^[AT]GG
1070 CCTGG
Cac8I 1174 GCTTGC GC[ACGT]^[ACGT]GC
1341 GCTTGC
EcoRII 618 CCAGG ^CC[AT]GG
1070 CCTGG
HgaI 34 GACGC GACGC(5/10)
825 GCGTC
HindIII 650 AAGCTT A^AGCTT
851 AAGCTT
MaeII 795 ACGT A^CGT
1334 ACGT
MslI 349 CACCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1054 CATTATGATG
MvaI 618 CCAGG CC^[AT]GG
1070 CCTGG
NlaIV 96 GGATCC GG[ACGT]^[ACGT]CC
253 GGGACC
SapI 679 GAAGAGC GCTCTTC(1/4)
106 GCTCTTC
SspBI 2 TGTACA T^GTACA
1262 TGTACA
SspI 517 AATATT AAT^ATT
672 AATATT
TaqI 67 TCGA T^CGA
1092 TCGA
TfiI 427 GAATC G^A[AT]TC
807 GATTC
TthHB8I 67 TCGA T^CGA
1092 TCGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BfaI BmyI BsaJI Bsp1286I BsrI
BssKI BstF5I BstYI Cfr13I DdeI
DpnII EarI FokI HinfI HphI
Ksp632I MaeI MboI MboII MflI
MnlI MseI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfaNI
Tru9I Tsp509I TspRI XhoII