Restriction map for
pF1KB3720
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MscI MseI MunI
NaeI NarI NciI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 908 TCCGGA T^CCGGA
AcsI 376 AAATTC [GA]^AATT[CT]
AlwI 180 GGATC GGATC(4/5)
AlwNI 553 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 938 GGGCCC GGGCC^C
ApoI 376 AAATTC [GA]^AATT[CT]
AvaI 635 CCCGAG C^[CT]CG[GA]G
AviII 287 TGCGCA TGC^GCA
AvrII 958 CCTAGG C^CTAGG
BlnI 958 CCTAGG C^CTAGG
BsaAI 619 TACGTG [CT]AC^GT[GA]
BseAI 908 TCCGGA T^CCGGA
BsmAI 239 GAGAC GTCTC(1/5)
BsmI 415 GAATGC GAATGC(1/-1)
BsoBI 635 CCCGAG C^[CT]CG[GA]G
BspEI 908 TCCGGA T^CCGGA
BsrBI 478 GAGCGG CCGCTC(-3/-3)
BsrDI 819 CATTGC GCAATG(2/0)
BssHII 11 GCGCGC G^CGCGC
DpnII 181 GATC ^GATC
FspI 287 TGCGCA TGC^GCA
MboI 181 GATC ^GATC
MboII 649 GAAGA GAAGA(8/7)
MroI 908 TCCGGA T^CCGGA
MspA1I 837 CAGCTG C[AC]G^C[GT]G
NcoI 534 CCATGG C^CATGG
NdeII 181 GATC ^GATC
PflMI 360 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 921 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 837 CAGCTG CAG^CTG
Sau3AI 181 GATC ^GATC
SbfI 549 CCTGCAGG CCTGCA^GG
ScaI 293 AGTACT AGT^ACT
SexAI 107 ACCTGGT A^CC[AT]GGT
SfiI 705 GGCCCACAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 549 CCTGCAGG CCTGCA^GG
TaqI 413 TCGA T^CGA
Tsp509I 377 AATT ^AATT
TthHB8I 413 TCGA T^CGA
Van91I 360 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 884 CCAACAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 10 CGCG CG^CG
12 CGCG
BanI 739 GGCACC G^G[CT][GA]CC
842 GGCACC
BanII 873 GAGCCC G[GA]GC[CT]^C
938 GGGCCC
BglI 61 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
706 GCCCACAAGGC
BsaWI 134 TCCGGT [AT]^CCGG[AT]
908 TCCGGA
BsgI 155 CTGCAC GTGCAG(16/14)
222 CTGCAC
BstUI 10 CGCG CG^CG
12 CGCG
BstXI 23 CCAGGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
489 CCAGGCTTTTGG
HapII 135 CCGG C^CGG
909 CCGG
HpaII 135 CCGG C^CGG
909 CCGG
MspI 135 CCGG C^CGG
909 CCGG
MvnI 10 CGCG CG^CG
12 CGCG
NdeI 695 CATATG CA^TATG
808 CATATG
NheI 253 GCTAGC G^CTAGC
663 GCTAGC
PstI 550 CTGCAG CTGCA^G
673 CTGCAG
Tsp45I 526 GTCAC ^GT[GC]AC
830 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeI MaeII MaeIII MnlI
MslI MvaI MwoI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
SfcI StyI TseI TspRI