Restriction map for
pF1KB3705
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccII AcyI AgeI AocI
Aor51HI ApaI AscI AspI AvrII
BbeI BbrPI BbvCI BciVI BlnI
BsaAI BsaHI BsaI BsgI BsiEI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstUI
Bsu36I ClaI CpoI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FseI HgaI Hin1I HindIII HpaI
KasI KspI MluI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 135 GGTACC G^GTACC
AccI 2365 GTATAC GT^[AC][GT]AC
AccIII 806 TCCGGA T^CCGGA
AclI 689 AACGTT AA^CGTT
AflII 3070 CTTAAG C^TTAAG
AflIII 623 ACGTGT A^C[GA][CT]GT
AhdI 2269 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2044 GTGCAC G^TGCAC
AlwNI 1774 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2044 GTGCAC G^TGCAC
Asp718I 135 GGTACC G^GTACC
AspEI 2269 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 2682 GGACC G^G[AT]CC
AviII 455 TGCGCA TGC^GCA
BalI 2790 TGGCCA TGG^CCA
BanI 135 GGTACC G^G[CT][GA]CC
BcgI 1684 CGACAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3070 CTTAAG C^TTAAG
BglII 3205 AGATCT A^GATCT
BlpI 1765 GCTCAGC GC^T[ACGT]AGC
BpmI 1361 CTGGAG CTGGAG(16/14)
Bpu1102I 1765 GCTCAGC GC^T[ACGT]AGC
BsaWI 806 TCCGGA [AT]^CCGG[AT]
BseAI 806 TCCGGA T^CCGGA
BseRI 268 GAGGAG GAGGAG(10/8)
BspEI 806 TCCGGA T^CCGGA
BsrDI 2603 GCAATG GCAATG(2/0)
BsrFI 2939 GCCGGT [GA]^CCGG[CT]
Bst1107I 2365 GTATAC GTA^TAC
BstBI 443 TTCGAA TT^CGAA
BstXI 86 CCATACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2365 GTATAC GTA^TAC
CelII 1765 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2939 GCCGGT [GA]^CCGG[CT]
DraII 1814 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2269 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1814 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 2742 CCTTGG C^C[AT][AT]GG
FauI 752 GCGGG CCCGC(4/6)
FspI 455 TGCGCA TGC^GCA
KpnI 135 GGTACC GGTAC^C
MluNI 2790 TGGCCA TGG^CCA
MroI 806 TCCGGA T^CCGGA
MscI 2790 TGGCCA TGG^CCA
NspV 443 TTCGAA TT^CGAA
Psp1406I 689 AACGTT AA^CGTT
SacI 1084 GAGCTC GAGCT^C
SapI 2796 GCTCTTC GCTCTTC(1/4)
SfuI 443 TTCGAA TT^CGAA
SmaI 1808 CCCGGG CCC^GGG
StyI 2742 CCTTGG C^C[AT][AT]GG
XbaI 1969 TCTAGA T^CTAGA
XcmI 2802 CCACATTGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1808 CCCGGG C^CCGGG
XmaI 1808 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AseI 1165 ATTAAT AT^TAAT
1258 ATTAAT
AsnI 1165 ATTAAT AT^TAAT
1258 ATTAAT
Asp700I 962 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2991 GAATCTCTTC
AvaI 140 CTCGGG C^[CT]CG[GA]G
1808 CCCGGG
BamHI 423 GGATCC G^GATCC
1632 GGATCC
BanII 827 GAGCCC G[GA]GC[CT]^C
1084 GAGCTC
BbsI 290 GAAGAC GAAGAC(2/6)
1386 GTCTTC
BclI 777 TGATCA T^GATCA
1131 TGATCA
BfaI 1819 CTAG C^TAG
1970 CTAG
BglI 895 GCCATAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2352 GCCTTTGTGGC
BpuAI 290 GAAGAC GAAGAC(2/6)
1386 GTCTTC
BsaBI 117 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2962 GATGCATATC
BsmI 853 GAATGC GAATGC(1/-1)
2184 GAATGC
BsoBI 140 CTCGGG C^[CT]CG[GA]G
1808 CCCGGG
BspMI 2270 ACCTGC ACCTGC(4/8)
2133 GCAGGT
EaeI 2790 TGGCCA [CT]^GGCC[GA]
2937 TGGCCG
EcoT22I 2224 ATGCAT ATGCA^T
2963 ATGCAT
FbaI 777 TGATCA T^GATCA
1131 TGATCA
HaeII 1776 GGCGCT [GA]GCGC^[CT]
3141 GGCGCT
HincII 2442 GTTGAC GT[CT]^[GA]AC
3021 GTTGAC
HindII 2442 GTTGAC GT[CT]^[GA]AC
3021 GTTGAC
MaeI 1819 CTAG C^TAG
1970 CTAG
MamI 117 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2962 GATGCATATC
MfeI 1351 CAATTG C^AATTG
2576 CAATTG
MslI 87 CATACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2115 CATGAAAGTG
MunI 1351 CAATTG C^AATTG
2576 CAATTG
NsiI 2224 ATGCAT ATGCA^T
2963 ATGCAT
NspI 241 GCATGT [GA]CATG^[CT]
1473 GCATGT
PshBI 1165 ATTAAT AT^TAAT
1258 ATTAAT
SmlI 1753 CTTGAG C^T[CT][GA]AG
3070 CTTAAG
SspI 1168 AATATT AAT^ATT
1371 AATATT
XmnI 962 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2991 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII