Restriction map for
pF1KB3699
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccI AccII AcyI
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BbrPI BbvCI
BciVI BclI BglII BlnI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
DrdI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MluI MvnI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 1118 TCCGGA T^CCGGA
AclI 1223 AACGTT AA^CGTT
AflII 2134 CTTAAG C^TTAAG
AhdI 229 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 813 CCTGAGG CC^T[ACGT]AGG
AspEI 229 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1776 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2472 TGGCCA TGG^CCA
BcgI 1895 CGAAGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2134 CTTAAG C^TTAAG
BglI 1637 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2091 GGTCTC GGTCTC(1/5)
BsaWI 1118 TCCGGA [AT]^CCGG[AT]
BseAI 1118 TCCGGA T^CCGGA
BsmBI 2499 CGTCTC CGTCTC(1/5)
BspEI 1118 TCCGGA T^CCGGA
BspMI 1831 ACCTGC ACCTGC(4/8)
BsrDI 1058 GCAATG GCAATG(2/0)
BsrFI 1760 GCCGGC [GA]^CCGG[CT]
BstXI 881 CCAAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 813 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1760 GCCGGC [GA]^CCGG[CT]
DraIII 1262 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 229 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 813 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2301 ATGCAT ATGCA^T
HaeII 1757 AGCGCC [GA]GCGC^[CT]
HindIII 2015 AAGCTT A^AGCTT
MluNI 2472 TGGCCA TGG^CCA
MroI 1118 TCCGGA T^CCGGA
MscI 2472 TGGCCA TGG^CCA
MslI 1849 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1760 GCCGGC GCC^GGC
NgoMIV 1760 GCCGGC G^CCGGC
NsiI 2301 ATGCAT ATGCA^T
PflFI 1776 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1223 AACGTT AA^CGTT
ScaI 98 AGTACT AGT^ACT
SmaI 2006 CCCGGG CCC^GGG
StuI 1530 AGGCCT AGG^CCT
Tth111I 1776 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2006 CCCGGG C^CCGGG
XmaI 2006 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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ApaI 371 GGGCCC GGGCC^C
1488 GGGCCC
AvaI 1162 CCCGAG C^[CT]CG[GA]G
2006 CCCGGG
BanI 1786 GGCACC G^G[CT][GA]CC
2181 GGTGCC
BsoBI 1162 CCCGAG C^[CT]CG[GA]G
2006 CCCGGG
BstAPI 252 GCACAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1826 GCATTACCTGC
BstEII 1201 GGTTACC G^GT[ACGT]ACC
2455 GGTGACC
BstPI 1201 GGTTACC G^GT[ACGT]ACC
2455 GGTGACC
DsaI 756 CCACGG C^C[GA][CT]GG
2483 CCGTGG
EcoO65I 1201 GGTTACC G^GT[ACGT]ACC
2455 GGTGACC
FauI 1917 CCCGC CCCGC(4/6)
2504 CCCGC
HgaI 1724 GACGC GACGC(5/10)
927 GCGTC
MaeII 1224 ACGT A^CGT
2498 ACGT
MfeI 376 CAATTG C^AATTG
1388 CAATTG
MunI 376 CAATTG C^AATTG
1388 CAATTG
NspI 968 GCATGC [GA]CATG^[CT]
1553 GCATGC
PflMI 183 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
756 CCACGGGTTGG
PpuMI 227 AGGACCC [GA]G^G[AT]CC[CT]
2337 AGGACCT
SacI 743 GAGCTC GAGCT^C
2194 GAGCTC
SmlI 1000 CTCAAG C^T[CT][GA]AG
2134 CTTAAG
SphI 968 GCATGC GCATG^C
1553 GCATGC
Van91I 183 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
756 CCACGGGTTGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BamHI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII