Restriction map for
pF1KB3690
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AgeI AocI Aor51HI AscI
Asp718I AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 934 GTAGAC GT^[AC][GT]AC
AclI 71 AACGTT AA^CGTT
AcyI 576 GGCGCC G[GA]^CG[CT]C
AfaI 567 GTAC GT^AC
AhdI 619 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 528 GTGCAC G^TGCAC
ApaI 104 GGGCCC GGGCC^C
ApaLI 528 GTGCAC G^TGCAC
AseI 327 ATTAAT AT^TAAT
AsnI 327 ATTAAT AT^TAAT
AspEI 619 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 528 GTGCAC G[AT]GC[AT]^C
AvaI 675 CCCGGG C^[CT]CG[GA]G
BanI 576 GGCGCC G^G[CT][GA]CC
BanII 104 GGGCCC G[GA]GC[CT]^C
BbeI 576 GGCGCC GGCGC^C
BbsI 1167 GTCTTC GAAGAC(2/6)
BciVI 795 GTATCC GTATCC(6/5)
BclI 693 TGATCA T^GATCA
BglII 885 AGATCT A^GATCT
BpuAI 1167 GTCTTC GAAGAC(2/6)
BsaAI 553 TACGTG [CT]AC^GT[GA]
BsaHI 576 GGCGCC G[GA]^CG[CT]C
BsgI 486 CTGCAC GTGCAG(16/14)
BsiHKAI 528 GTGCAC G[AT]GC[AT]^C
BsmFI 1251 GGGAC GGGAC(10/14)
BsmI 426 GCATTC GAATGC(1/-1)
BsoBI 675 CCCGGG C^[CT]CG[GA]G
BspDI 1173 ATCGAT AT^CGAT
BsrI 1020 CCAGT ACTGG(1/-1)
CfoI 577 GCGC GCG^C
ClaI 1173 ATCGAT AT^CGAT
Eam1105I 619 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 680 GAAGAG CTCTTC(1/4)
EcoT14I 108 CCAAGG C^C[AT][AT]GG
FauI 979 CCCGC CCCGC(4/6)
FbaI 693 TGATCA T^GATCA
HaeII 576 GGCGCC [GA]GCGC^[CT]
HhaI 577 GCGC GCG^C
Hin1I 576 GGCGCC G[GA]^CG[CT]C
HinP1I 577 GCGC G^CGC
KasI 576 GGCGCC G^GCGCC
Ksp632I 680 GAAGAG CTCTTC(1/4)
MslI 468 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 576 GGCGCC GG^CGCC
NspI 1038 ACATGC [GA]CATG^[CT]
PleI 49 GACTC GAGTC(4/5)
PpuMI 1251 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1228 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 327 ATTAAT AT^TAAT
Psp1406I 71 AACGTT AA^CGTT
RsaI 567 GTAC GT^AC
SfcI 932 CTGTAG C^T[GA][CT]AG
SfoI 576 GGCGCC GGC^GCC
SmaI 675 CCCGGG CCC^GGG
StyI 108 CCAAGG C^C[AT][AT]GG
XbaI 291 TCTAGA T^CTAGA
XcmI 399 CCAGATGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 675 CCCGGG C^CCGGG
XmaI 675 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 980 CCGC CCGC(-3/-1)
924 GCGG
Asp700I 295 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
817 GAACTCTTTC
AvaII 654 GGTCC G^G[AT]CC
1252 GGACC
BfaI 292 CTAG C^TAG
461 CTAG
BmyI 104 GGGCCC G[AGT]GC[ACT]^C
528 GTGCAC
BsmAI 683 GAGAC GTCTC(1/5)
857 GAGAC
Bsp1286I 104 GGGCCC G[AGT]GC[ACT]^C
528 GTGCAC
Cac8I 526 GCGTGC GC[ACGT]^[ACGT]GC
1212 GCTGGC
DraII 103 GGGGCCC [GA]G^G[ACGT]CC[CT]
1251 GGGACCC
EcoO109I 103 GGGGCCC [GA]G^G[ACGT]CC[CT]
1251 GGGACCC
HgaI 1209 GACGC GACGC(5/10)
948 GCGTC
HphI 558 GGTGA GGTGA(8/7)
951 TCACC
MaeI 292 CTAG C^TAG
461 CTAG
MfeI 92 CAATTG C^AATTG
1043 CAATTG
MunI 92 CAATTG C^AATTG
1043 CAATTG
MwoI 199 GCAAAGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
517 GCCCGGACAGC
TfiI 803 GAATC G^A[AT]TC
847 GAATC
XmnI 295 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
817 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
BbvI BcnI BsaJI BsiYI BslI
BspHI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI NciI NdeII NlaIII NlaIV
PvuII RcaI Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII