Restriction map for
pF1KB3682
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Aor51HI
ApaI AscI Asp718I AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Cfr10I ClaI
CpoI DraI EaeI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FauI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MflI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI StuI SwaI
Tth111I XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 1565 ACATGT A^C[GA][CT]GT
Alw44I 1481 GTGCAC G^TGCAC
AlwI 774 GGATC GGATC(4/5)
AlwNI 100 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2188 CCTGAGG CC^T[ACGT]AGG
ApaLI 1481 GTGCAC G^TGCAC
AseI 700 ATTAAT AT^TAAT
AsnI 700 ATTAAT AT^TAAT
BciVI 2007 GTATCC GTATCC(6/5)
BlpI 1492 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1492 GCTCAGC GC^T[ACGT]AGC
BsmBI 1351 GAGACG CGTCTC(1/5)
BsmI 690 GAATGC GAATGC(1/-1)
BspHI 177 TCATGA T^CATGA
BspLU11I 1565 ACATGT A^CATGT
BssSI 550 CACGAG CACGAG(-5/-1)
Bsu36I 2188 CCTGAGG CC^T[ACGT]AGG
CelII 1492 GCTCAGC GC^T[ACGT]AGC
CfoI 740 GCGC GCG^C
DraIII 1475 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 433 GACAGTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1288 GAAGAG CTCTTC(1/4)
Eco81I 2188 CCTGAGG CC^T[ACGT]AGG
EcoRI 2230 GAATTC G^AATTC
EcoT14I 86 CCATGG C^C[AT][AT]GG
HaeII 739 AGCGCC [GA]GCGC^[CT]
HhaI 740 GCGC GCG^C
HinP1I 740 GCGC G^CGC
HindIII 1579 AAGCTT A^AGCTT
Ksp632I 1288 GAAGAG CTCTTC(1/4)
MaeII 1385 ACGT A^CGT
NcoI 86 CCATGG C^CATGG
PaeR7I 1348 CTCGAG C^TCGAG
PflMI 1415 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1014 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 700 ATTAAT AT^TAAT
RcaI 177 TCATGA T^CATGA
SapI 1288 GAAGAGC GCTCTTC(1/4)
SmaI 1826 CCCGGG CCC^GGG
SpeI 711 ACTAGT A^CTAGT
SphI 977 GCATGC GCATG^C
StyI 86 CCATGG C^C[AT][AT]GG
Van91I 1415 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2149 TCTAGA T^CTAGA
XhoI 1348 CTCGAG C^TCGAG
XmaCI 1826 CCCGGG C^CCGGG
XmaI 1826 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 181 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACGAGGTGTC
AspEI 181 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACGAGGTGTC
BanI 1220 GGCACC G^G[CT][GA]CC
1252 GGCACC
BbsI 292 GAAGAC GAAGAC(2/6)
1011 GAAGAC
BcgI 1355 CGACCTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
816 GCAGCAAGTTCG
BpmI 2049 CTCCAG CTGGAG(16/14)
49 CTGGAG
BpuAI 292 GAAGAC GAAGAC(2/6)
1011 GAAGAC
BseRI 1183 GAGGAG GAGGAG(10/8)
2191 GAGGAG
BsrBI 1401 GAGCGG CCGCTC(-3/-3)
1858 GAGCGG
BstXI 557 CCAATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2028 CCATGCCTTTGG
DraII 1269 GGGTCCT [GA]G^G[ACGT]CC[CT]
1795 GGGACCT
DsaI 86 CCATGG C^C[GA][CT]GG
1618 CCGTGG
Eam1105I 181 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACGAGGTGTC
EcoNI 48 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1773 CCTGTTGAAGG
EcoO109I 1269 GGGTCCT [GA]G^G[ACGT]CC[CT]
1795 GGGACCT
HapII 1792 CCGG C^CGG
1827 CCGG
HpaII 1792 CCGG C^CGG
1827 CCGG
MfeI 1228 CAATTG C^AATTG
1318 CAATTG
MspI 1792 CCGG C^CGG
1827 CCGG
MunI 1228 CAATTG C^AATTG
1318 CAATTG
PpuMI 1269 GGGTCCT [GA]G^G[AT]CC[CT]
1795 GGGACCT
PstI 781 CTGCAG CTGCA^G
814 CTGCAG
SacI 948 GAGCTC GAGCT^C
1821 GAGCTC
SfaNI 1685 GCATC GCATC(5/9)
1736 GCATC
SmlI 601 CTCAAG C^T[CT][GA]AG
1348 CTCGAG
TaqI 1349 TCGA T^CGA
2234 TCGA
TfiI 1452 GAATC G^A[AT]TC
1989 GAATC
TthHB8I 1349 TCGA T^CGA
2234 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI AvaI AvaII BanII
BbvI BclI BcnI BfaI BmyI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SspI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XmnI