Restriction map for
pF1KB3681
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbrPI BclI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 131 CGCG CG^CG
AfaI 2499 GTAC GT^AC
AflII 1594 CTTAAG C^TTAAG
AflIII 2570 ACACGT A^C[GA][CT]GT
AgeI 2622 ACCGGT A^CCGGT
Alw44I 2145 GTGCAC G^TGCAC
AocI 2377 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1793 AGCGCT AGC^GCT
ApaLI 2145 GTGCAC G^TGCAC
BbvCI 2490 CCTCAGC CCTCAGC(-5/-2)
BcgI 1465 GCAGAGCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 631 GTATCC GTATCC(6/5)
BfrI 1594 CTTAAG C^TTAAG
BsaI 886 GAGACC GGTCTC(1/5)
BssHII 130 GCGCGC G^CGCGC
BstUI 131 CGCG CG^CG
Bsu36I 2377 CCTCAGG CC^T[ACGT]AGG
EagI 1241 CGGCCG C^GGCCG
EarI 1045 GAAGAG CTCTTC(1/4)
EclXI 1241 CGGCCG C^GGCCG
Eco47III 1793 AGCGCT AGC^GCT
Eco52I 1241 CGGCCG C^GGCCG
Eco81I 2377 CCTCAGG CC^T[ACGT]AGG
Ksp632I 1045 GAAGAG CTCTTC(1/4)
MvnI 131 CGCG CG^CG
NaeI 81 GCCGGC GCC^GGC
NcoI 1407 CCATGG C^CATGG
NgoMIV 81 GCCGGC G^CCGGC
PaeR7I 1801 CTCGAG C^TCGAG
PinAI 2622 ACCGGT A^CCGGT
PvuII 2493 CAGCTG CAG^CTG
RsaI 2499 GTAC GT^AC
SfiI 2551 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2621 CACCGGTG C[GA]^CCGG[CT]G
SphI 2009 GCATGC GCATG^C
TfiI 2659 GAATC G^A[AT]TC
Tsp509I 2032 AATT ^AATT
XcmI 1484 CCATCCGCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1801 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 25 GGCGCC G[GA]^CG[CT]C
70 GGCGCC
BamHI 168 GGATCC G^GATCC
326 GGATCC
BbeI 25 GGCGCC GGCGC^C
70 GGCGCC
BbsI 1927 GAAGAC GAAGAC(2/6)
2467 GTCTTC
BglI 2245 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2552 GCCGGGCGGGC
BpuAI 1927 GAAGAC GAAGAC(2/6)
2467 GTCTTC
BsaHI 25 GGCGCC G[GA]^CG[CT]C
70 GGCGCC
BsaWI 323 ACCGGA [AT]^CCGG[AT]
2622 ACCGGT
BsiEI 1241 CGGCCG CG[GA][CT]^CG
1650 CGACCG
BsmI 1476 GAATGC GAATGC(1/-1)
1502 GAATGC
BspMI 500 ACCTGC ACCTGC(4/8)
889 ACCTGC
BsrDI 1128 CATTGC GCAATG(2/0)
1325 CATTGC
BstXI 584 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
981 CCACCGGCCTGG
DraIII 2001 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2170 CACGGGGTG
DsaI 1254 CCACGG C^C[GA][CT]GG
1407 CCATGG
EcoNI 1014 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1182 CCTGGATCAGG
EcoT22I 1 ATGCAT ATGCA^T
1504 ATGCAT
Hin1I 25 GGCGCC G[GA]^CG[CT]C
70 GGCGCC
HincII 1003 GTTGAC GT[CT]^[GA]AC
1101 GTTGAC
HindII 1003 GTTGAC GT[CT]^[GA]AC
1101 GTTGAC
KasI 25 GGCGCC G^GCGCC
70 GGCGCC
MaeIII 1010 GTGAC ^GT[ACGT]AC
1999 GTCAC
MslI 1471 CACTCGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2004 CATGTGCATG
NarI 25 GGCGCC GG^CGCC
70 GGCGCC
NsiI 1 ATGCAT ATGCA^T
1504 ATGCAT
PleI 94 GACTC GAGTC(4/5)
1090 GAGTC
PshAI 406 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1085 GACAGGAGTC
SfoI 25 GGCGCC GGC^GCC
70 GGCGCC
Tsp45I 1010 GTGAC ^GT[GC]AC
1999 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PpuMI
PstI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI Tru9I TseI TspRI
Tth111I TthHB8I Van91I XhoII XmaCI
XmaI